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Abdulah, N. S, Al-Hejjaj, M. Y. (1401). The Relative of Spa Gene Types, Prevalence and Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus. سامانه مدیریت نشریات علمی, 77(6), 2423-2430. doi: 10.22092/ari.2022.358867.2320
N. S Abdulah; M. Y Al-Hejjaj. "The Relative of Spa Gene Types, Prevalence and Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus". سامانه مدیریت نشریات علمی, 77, 6, 1401, 2423-2430. doi: 10.22092/ari.2022.358867.2320
Abdulah, N. S, Al-Hejjaj, M. Y. (1401). 'The Relative of Spa Gene Types, Prevalence and Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus', سامانه مدیریت نشریات علمی, 77(6), pp. 2423-2430. doi: 10.22092/ari.2022.358867.2320
Abdulah, N. S, Al-Hejjaj, M. Y. The Relative of Spa Gene Types, Prevalence and Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus. سامانه مدیریت نشریات علمی, 1401; 77(6): 2423-2430. doi: 10.22092/ari.2022.358867.2320

The Relative of Spa Gene Types, Prevalence and Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus

Archives of Razi Institute
مقاله 50، دوره 77، شماره 6، بهمن و اسفند 2022، صفحه 2423-2430    اصل مقاله (424.15 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358867.2320
نویسندگان
N. S Abdulah* ؛ M. Y Al-Hejjaj
Department of Microbiology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq
چکیده
Methicillin-resistant Staphylococcus aureus (MRSA) is the primary cause of nosocomial and animal, community-acquired infections. S. aureus is a common inhabitant of the upper respiratory tract. Molecular typing methods are vital for investigations of MRSA. The MRSA has significantly increased in Iraq. This study aimed to determine the prevalence of this bacteria and know the distribution of Spa type among antibiotics-resistant local isolates from different sources. A total of 150 samples were collected from three different sources: humans, animals and the environment. Among all these samples, 55 MRSA isolates were determined using the phenotypic method and the mecA gene. Antibiotic resistance profiles were screened using the disc diffusion method. Whereas Spa types were identified by using PCR technique and nucleotide sequencing analysis. The MRSA presence rates were 67.5%, 80 %, and 31.3% in bovine, human, and environmental sources, respectively. The highest sensitivity of MRSA was to vancomycin, and the lowest was to penicillin. Multi-drug resistance was found to be in all isolates. Molecular investigation showed that 100% of the tested MRSA isolates harboured a Spa gene; Spa gene typing assay reveals that the most repetitive spa type was t304, t8986, and t14870, which were reported in humans and animals, followed by type t 304 t14870 in environmental isolates. This study's findings could help identify the genetic variants responsible for the emergence and spread of these bacteria in the region.
کلیدواژه‌ها
Spa Gene Types؛ Prevalence؛ Antibiotic Resistance
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مراجع
  1. Guo Y, Song G, Sun M, Wang J, Wang Y. Prevalence and therapies of antibiotic-resistance in Staphylococcus aureus. Front Cell Infect Microbiol. 2020;10:107.
  2. Al-kaabi KT, Al-Kashwan TAJ, Al-Fahham HR. Molecular Study of Methicillin Resistant Staphylococcus aureus Isolated from Different Hospitals in Najaf-Iraq. Hospital. 2016;35(8):22.
  3. Gadban TH, Al-Amara SS, Jasim HA. Screening the frequency of panton-valentine leukocidin (pvl) gene between methicillin resistant Staphylococcus aureus isolated from diabetic foot patients in Al-Basrah governorate, south of Iraq. Sys Rev Pharm. 2020;11(11):285-90.
  4. Hattab SO, Al-Amara SS. Panton-valentine leukocidin (pvl) gene prevalence among methicillin-resistant Staphylococcus aureus isolated from oral infection in Basrah Governoratel-Basrah, Iraq. J Basrah Res. 2021;47(1).
  5. Mazaal MA, Ibrahim HK, Mater AD. Molecular detection of nuc and sea genes of staphylococcus aureus isolated from cow and sheep meat in basrah city. Basrah J Vet Res. 2021;20(1).
  6. Mezban JM, Khudor MH, Abbas BA. Multiplex pcr detection of erythromycin resistance genes in coagulase negative staphylococci isolated from cows in basrah, Iraq. Basrah J Vet Res. 2018;17(1).
  7. Sheehan AAA, Khudor MH, Isihak FA. Some immunological responses in rats injected with prepared bacterin toxoid of local methicillin resistant Staphylococcus aureus. Iraqi J Vet Med. 2022;36(2):401-6.
  8. Cikman A, Aydin M, Gulhan B, Karakecili F, Kurtoglu MG, Yuksekkaya S, et al. Absence of the mecC gene in methicillin-resistant Staphylococcus aureus isolated from various clinical samples: The first multi-centered study in Turkey. J Infect Public Health. 2019;12(4):528-33.
  9. Bien J, Sokolova O, Bozko P. Characterization of virulence factors of Staphylococcus aureus: novel function of known virulence factors that are implicated in activation of airway epithelial proinflammatory response. J Pathog. 2011;2011.
  10. Strommenger B, Braulke C, Heuck D, Schmidt C, Pasemann B, Nubel U, et al. spa typing of Staphylococcus aureus as a frontline tool in epidemiological typing. J Clin Microbiol. 2008;46(2):574-81.
  11. Arakere G, Nadig S, Swedberg Gt, Macaden R, Amarnath SK, Raghunath D. Genotyping of methicillin-resistant Staphylococcus aureus strains from two hospitals in Bangalore, South India. J Clin Microbiol. 2005;43(7):3198-202.
  12. McClure J-A, Conly JM, Lau V, Elsayed S, Louie T, Hutchins W, et al. Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from-resistant staphylococci. J Clin Microbiol. 2006;44(3):1141-4.
  13. Havaei SA, Moghim S, Bardebari AM, Narimani T, Azimian A, Akbari M. The comparison of Staphylococcus aureus types 5 and 8 with respect to methicillin resistance in patients admitted to Al-Zahra Hospital by PCR. Adv Biomed Res. 2013;2(1):13.
  1. Votintseva AA, Fung R, Miller RR, Knox K, Godwin H, Wyllie DH, et al. Prevalence of Staphylococcus aureus protein A (spa) mutants in the community and hospitals in Oxfordshire. BMC Microbiol. 2014;14(1):1-11.
  2. Khudaier BY, Abbas BA, Khudaier AM. Detection of Methicillin Resistant Staphylococcus aureus Isolated from Human and Animals in Basrah Province/Iraq. MRVSA2013. 2013;2:12-21.
  3. Enany ME, Algammal AM, Shagar GI, Hanora AM, Elfeil WK, Elshaffy NM. Molecular typing and evaluation of Sidr honey inhibitory effect on virulence genes of MRSA strains isolated from catfish in Egypt. Pak J Pharm Sci. 2018;31(5).
  4. Singh G, Broor S, Agarwal P. Molecular characterisation of Staphylococcus aureus using spa typing as a diagnostic tool in Haryana, India. Indian J Med Microbiol. 2018;36(1):26-31.
  5. Karthik L, Kumar G, Keswani T, Bhattacharyya A, Chandar SS, Bhaskara Rao K. Protease inhibitors from marine actinobacteria as a potential source for antimalarial compound. PloS One. 2014;9(3):e90972.
  6. Baum C, Haslinger-Löffler B, Westh H, Boye K, Peters G, Neumann C, et al. Non-spa-typeable clinical Staphylococcus aureus strains are naturally occurring protein A mutants. J Clin Microbiol. 2009;47(11):3624-9.
  7. Haggag MG, Aboelnour AE, Al-Kaffas M. MRSA screening and spa gene detection in isolates from healthcare workers at ophthalmology hospital in Egypt. Bull Natl Res Cent. 2019;43(1):1-7.
  8. Bartels M, Worning P, Andersen L, Bes M, Enger H, Ås C, et al. Repeated introduction and spread of the MRSA clone t304/ST6 in northern Europe. Clin Microbiol Infect. 2021;27(2):284. e1-. e5.
  9. Enger H, Larssen KW, Damås ES, Aamot HV, Blomfeldt A, Elstrøm P, et al. A tale of two STs: molecular and clinical epidemiology of MRSA t304 in Norway 2008–2016. Eur J Clin Microbiol Infect Dis. 2022;41(2):209-18.
  10. Asadollahi P, Farahani NN, Mirzaii M, Khoramrooz SS, Van Belkum A, Asadollahi K, et al. Distribution of the most prevalent spa types among clinical isolates of methicillin-resistant and-susceptible Staphylococcus aureus around the world: a review. Front Microbiol. 2018;9:163.
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