Najd Obaid, F, Al-Dabbagh, K. A, Salam Karim, Y, Abdallah Mahdi, O, Mahdi Rheima, A, Alkadir, O. K. A., Hamad, D. A, Mahmood Shihab, E, Hussin Alwan, N, Alwaily, E. R. (1401). Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq. سامانه مدیریت نشریات علمی, 77(3), 1269-1273. doi: 10.22092/ari.2022.358832.2317
F Najd Obaid; K. A Al-Dabbagh; Y Salam Karim; O Abdallah Mahdi; A Mahdi Rheima; O. K. A. Alkadir; D. A Hamad; E Mahmood Shihab; N Hussin Alwan; E. R Alwaily. "Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq". سامانه مدیریت نشریات علمی, 77, 3, 1401, 1269-1273. doi: 10.22092/ari.2022.358832.2317
Najd Obaid, F, Al-Dabbagh, K. A, Salam Karim, Y, Abdallah Mahdi, O, Mahdi Rheima, A, Alkadir, O. K. A., Hamad, D. A, Mahmood Shihab, E, Hussin Alwan, N, Alwaily, E. R. (1401). 'Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq', سامانه مدیریت نشریات علمی, 77(3), pp. 1269-1273. doi: 10.22092/ari.2022.358832.2317
Najd Obaid, F, Al-Dabbagh, K. A, Salam Karim, Y, Abdallah Mahdi, O, Mahdi Rheima, A, Alkadir, O. K. A., Hamad, D. A, Mahmood Shihab, E, Hussin Alwan, N, Alwaily, E. R. Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq. سامانه مدیریت نشریات علمی, 1401; 77(3): 1269-1273. doi: 10.22092/ari.2022.358832.2317
Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq
2Department of Dentistry, Al-Hadba University College, Mosul, Iraq
3Department of Pharmacy, Al-Manara College For Medical Sciences, Maysan, Iraq
4Anesthesia Techniques Department, Al-Mustaqbal University College, Babylon, Iraq
5College of Technical Engineering, The Islamic University, Najaf, Iraq
6Department of Optics Techniques, Dijlah University College, Al-Masafi Street, Al-Dora, Baghdad 00964, Iraq
7Al-Nisour University College, Baghdad, Iraq
8Nursing Department, Hilla University College, Babylon, Iraq
9Al-Esraa University College, Baghdad, Iraq
10Department of Nursing, Al-Zahrawi University College, Karbala, Iraq
11Microbiology Research Group , College of Pharmacy, Al-Ayen University, Thi-Qar, Iraq
چکیده
It has been approved that one of the most dangerous foodborne pathogenic bacteria is E. coli O157:H7, which is responsible for several infection and death cases worldwide.It is well documented that in the developing countries E. coli O157:H7 is considered the main causative pathogen of human gastrointestinal infections. Therefore, the current research was aimed to evaluate the prevalence of E. coli O157:H7 in dairy cattle's milk using a rapid method, in Iraq (Najaf, Baghdad, Kirkuk, and Erbil). Over a period of 6 months (During hot months) samples were obtained and investigated by culturing on selective media (CT-SMAC). The multiplex PCR (m-PCR) also used for milk sample direct investigation. Using biochemical tests the recorded data showed that, 2 recognized isolates were E. coli, while the recorded data obtained from m-PCR assay revealed that none of the isolated E. coli was toxigenic E.coli O157:H7. The results of m-PCR on the milk samples revealed that 45 milk samples contained at least one of the following genes: O157, H7, stx1, stx2 genes. Also the results of the m-PCR revealed that 2 samples (raw milk) were toxigenic O157:H7 positive. In conclusion, to the best of authors' knowledge, this investigation was the first report on the prevalence of E. coli O157:H7 in the raw milk samples in Iraq. The results showed that the proportion of contaminated milk samples contaminated with E. coli O157:H7 identified in the current survey were similar to that the results of the previously published research from different dairy products across different countries in the Middle East region.
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