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Study the ability of Salmonella typhimiurm to induce Cytokine TNFα in mice treated with olive leaves extract and Ciprofloxacin | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 05 خرداد 1401 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2022.358331.2196 | ||
| نویسندگان | ||
Duaa H. D. Al Mnshad  ؛ Abass T. Al Joudi؛ Layla Q.Jawad؛ Ruqaya H. M. Al –Hasnawi
| ||
| Al –Esraa College University, Baghdad, IRAQ | ||
| چکیده | ||
| TNF-α is one of cytokines group that produce by inflammatory cell while the main host defense against Salmonella species occurs through the neutrophils followed by mononuclear cells. These inflammatory cells produce cytokines such as TNF-α, IFN- γ, IL-1, and etc. Ciprofloxacin is the last drug of choice to clear the infection. Ciprofloxacin, a grace inhibitor, kills bacteria by inducing chromosome fragmentation and reactive oxygen species (ROS) in the bacterial cell. This study was designed to investigate the ability of Salmonella typhimiurm to induce TNFα in cells. 30 NMRI mice from both gander were divided randomly into 3 groups (n=10) and treated as follow: 1st group was intraperitoneally (IP) injected with 0.1 ml/4×108 CFU/ ml bacterial suspension of S. typhimiurm as a positive control . 2nd group was injected (IP) with S. typhimiurm 0.1 ml 4×108 CFU/ ml then injected with 30 mg/kg/0.3 ml of Ciprofloxacin intramuscular (IM). 3rdgroup pre-treated with olive leaf alcoholic extract by gavage-tube for 2 weeks then injected I/P 0.1 ml /4×108 CFU/ml S. typhimiurm, after that, post-treated with 30 mg/kg /0.3 ml of olive leaf extract orally by gavage-tube until the end of experimental (30 days). The recorded data from group 1 showed heavy deposition of TNFα marker in spleen, liver and examination showed dark brown cellular components. Also in 2nd group the tissue sections of spleen and liver showed heavy deposition of TNFα marker, other sections showed moderated deposition of TNFα marker. In 3rd group, tissue sections of spleen and liver showed either small or absence deposition of TNFα marker. Histopathological examination of infected 1st group liver and spleens' tissue section shows lobular hepatic necrosis with mononuclear cells aggregation appears as granuloma's lesions particularly lymphocyte in portal area around bile duct and blood vessels, with proliferation of macrophages which named kupffer cells depletion of white pulp in splenic tissue as compared with 2nd and 3rd group, which showed moderated lesion in infected and treated mice. As a conclusion, we can concluded that Olive leaves extract reduce the infection of Salmonella typhimiurm in the pre and post treated group better than Ciprofloxacin . | ||
| کلیدواژه‌ها | ||
| Salmonella typhimiurm , TNF&alpha؛ , antioxidants , Olive Leaves Extract | ||
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آمار تعداد مشاهده مقاله: 8 |
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