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آمار
| تعداد نشریات | 280 |
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Evaluation of gene expression of norA and norB gene in Ciproflaxin and Levofloxacin resistant Staphylococcus aureas | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 03 خرداد 1401 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2022.358335.2197 | ||
| نویسندگان | ||
Noor Saad Latteef  ؛ Warqaa Y. Salih؛ Alaa Aziz Abdulhassan؛ Rafal Jasim Obeed
| ||
| Institute of Genetic Engineering and Biotechnology for Post Graduate Studies, University of Baghdad, Baghdad, Iraq. | ||
| چکیده | ||
| The presence of efflux pumps genes in Staphylococcus aureus, such as norA and norB, is critical for ciprofloxacin and levofloxacin resistance. This study examined the efflux pump gene expression and activity in ciprofloxacin and levofloxacin resistant S. aureus strains. Twenty clinical samples of wounds and burns were collected. S. aureus strains were tested using specific culture media. Antibiotic susceptibility testing was done using the disc diffusion method . After determining disc diffusion method of ciprofloxacin and levofloxacin, Methicillin resistant Staphylococcus aureus (MRSA) isolates were found in ten of the twenty clinical samples. The susceptibility of S. aureus in the study revealed 40% ciprofloxacin resistance and 20% levofloxacin resistance. The gene expression of nor A and nor B efflux pump genes was assessed using Real-Time PCR. The nor A gene was detected in all ciprofloxacin-resistant pathogens, and norA gene expression increased in samples treated with ciprofloxacin as compared to samples not treated with ciprofloxacin, according to the results of a real-time PCR test. The nor B gene was detected in resistant strains, and its expression increased, as was the case with the nor A gene. The fold of gene expression of nor B gene for the ten isolates ranged from (12.082 to 42.81 fold) and also This result was higher than the fold of nor A gene (0.0036-34.05 fold). The research study discovered that efflux pump genes play a key role in ciprofloxacin and levofloxacin resistance. Also, when employed as a housekeeping gene in gene expression, the 16S rRNA gene produced great results. | ||
| کلیدواژه‌ها | ||
| gene expression؛ nor A and nor B؛ Staphylococcus aureus | ||
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آمار تعداد مشاهده مقاله: 22 |
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