1Al-Rafidain University College, Department of Dentist, Baghdad-Iraq
2Biology Department, College of Science, Mustansiriyah University, Baghdad-Iraq
32,3 Biology Department, College of Science, Mustansiriyah University, Baghdad-Iraq
چکیده
Acinetobacter baumannii (A. baumannii) is Gram negative bacteria that are non-lactose fermenting, characterized by a spherical rod shape (Coccobacilli, Aerobic bacteria), belonging to the family of Moraxellacea. A. baumannii is a pathogenic, opportunistic organism that infects humans in society and hospitals. Patients with immune system defects, in particular, are at risk, especially those with burn infections and patients hospitalized in intensive care (ICU). It plays an important role in many illnesses, including septicemia, pneumonia, meningitis, soft tissues, skin infection, endocarditis, and urinary tract infection (UTI). The current study included immunological evaluation of infection with A. baumannii. In the current study 150 blood samples were obtained as follows: 100 blood samples were collected from infected individuals with A. baumannii admitted to hospitals in Baghdad. 50 blood samples were obtained from healthy individuals and considered as the control. 10 ml of blood samples collected from the venous blood from the participants. A. baumannii was collected and isolated from infected patients and diagnosed by traditional methods, using different culture media (MacConkey agar, blood agar and Chromogenetic agar) and by biochemical assays, then the bacteria diagnosis was confirmed using the VITEK 2 ID-GN cards. Microscopic examination and culture diagnosis of bacteria were conducted, and the diagnosis was confirmed by complete biochemical examinations using VITEK2 Compact System. Assessments included the serum level of IL-17A and TNF-α for patients infected with A. baumannii who were hospitalized.
The study recorded a significant increase in the serum level of IL-17A for patients infected with A.baumannii (479.83 ± 26.21 pg/ml) compared to control subjects (69.32 ± 4.53 pg/ml). The recorded data showed a significant increase in the serum level of TNF-α for patients infected with A .baumannii (98.05 ± 28.89 pg/ml) compared to control (1.40 ± 25.12 pg/ml).