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Ali Mandeel, Haider, Shahid Jassim, Alaa, Kadhum Naeem, Ahlam. (1401). Prevalence of Enterohemorragic and Enteroaggregative E. coli among Children with Diarrhea in Najaf, Iraq. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2022.357755.2097
Haider Ali Mandeel; Alaa Shahid Jassim; Ahlam Kadhum Naeem. "Prevalence of Enterohemorragic and Enteroaggregative E. coli among Children with Diarrhea in Najaf, Iraq". سامانه مدیریت نشریات علمی, , , 1401, -. doi: 10.22092/ari.2022.357755.2097
Ali Mandeel, Haider, Shahid Jassim, Alaa, Kadhum Naeem, Ahlam. (1401). 'Prevalence of Enterohemorragic and Enteroaggregative E. coli among Children with Diarrhea in Najaf, Iraq', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2022.357755.2097
Ali Mandeel, Haider, Shahid Jassim, Alaa, Kadhum Naeem, Ahlam. Prevalence of Enterohemorragic and Enteroaggregative E. coli among Children with Diarrhea in Najaf, Iraq. سامانه مدیریت نشریات علمی, 1401; (): -. doi: 10.22092/ari.2022.357755.2097

Prevalence of Enterohemorragic and Enteroaggregative E. coli among Children with Diarrhea in Najaf, Iraq

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مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 31 اردیبهشت 1401  XML
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357755.2097
نویسندگان
Haider Ali Mandeel email 1؛ Alaa Shahid Jassim2؛ Ahlam Kadhum Naeem3
1Ministry of Education, General Directorate of Education in Alqadssya, Iraq
2Ministry of Education, General Directorate of Education in Najaf, Iraq
3Kufa University, Faculty of Education for Girls, Department of Biology/Iraq
چکیده
Diarrhea is one of the leading causes of morbidity and mortality among children under 5 years in the developing countries. Classification of diarrheagenic E. coli (DEC) strains among children with diarrhea is still take low attention. The present study aimed to determine the prevalence of Enterohaemorragic E. coli (EHEC) and Enteroaggregative E. coli (EAEC) among children under 5 years old suffering from acute diarrhea. Stool samples (100 samples) have been collected from children under 5 years old suffering from acute diarrhea for molecular detection of EHEC (using stx1 and stx2) and EAEC using (aat) by PCR technique. The results showed a high percentage of isolation of EHEC from stool samples in compression with EAEC where 15.9% of 75 identified DEC isolates were belonging to EHEC while 5.3% were belong to EAEC. Among EHEC, stx1 was high prevalence among isolates (9.3%) compared with stx2 which showed low percentage (6.6%). A high frequency of EHEC detected in male at age group 7-12 months while EAEC detected in female with age group 13-19 months. In conclusion EHEC and EAEC were associated with bloody and watery diarrhea among children under 5 years old. Gene associated with virulence factors (stx1, stx2, and aat)could be used as genetic marker for detection of EHAE and EAEC.
کلیدواژه‌ها
EAEC؛ stx؛ aat؛ diarrhea؛ PCR
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تعداد مشاهده مقاله: 14


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