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اثر کود کامل NPK، بوتامیسول و اسید هیومیک بر صفات مورفوفیزیولوژیک و اسانس سه گونه از جنس آویشن (Thymus spp.) تحت تنش خشکی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 9، دوره 38، شماره 2 - شماره پیاپی 112، تیر 1401، صفحه 301-321 اصل مقاله (1.54 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2022.357303.3119 | ||
| نویسنده | ||
| مهراب یادگاری* | ||
| دانشیار، مرکز تحقیقات گیاهان دارویی ادویه‌ای و عطری، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران | ||
| چکیده | ||
| با توجه به نقش ارزنده گیاه آویشن در صنایع داروسازی و غذایی کشور، در این تحقیق اثرگذاری کودهای کامل NPK، بوتامیسول و اسید هیومیک بر خصوصیات مورفوفیزیولوژیکی و اسانس سه گونه آویشن کوهی (Thymus kotschyanus Boiss)، آویشن باغی (T. vulgaris L.) و آویشن دنایی (T. daenensis Celark) تحت شرایط تنش خشکی مطالعه گردید. این تحقیق در سه آزمایش جداگانه به‌صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با چهار تکرار در سال زراعی 1399-1400 در دانشگاه آزاد اسلامی واحد شهرکرد انجام شد. محلول‌پاشی دو بار با فاصله زمانی سه هفته بعد از مرحله 10 برگی شدن گیاه انجام شد. 24 تیمار آزمایشی شامل عدم استفاده (A1) و استفاده (A2) از NPK، عدم استفاده (B1) و استفاده (B2) از اسید هیومیک، عدم استفاده (C1) و استفاده (C2) از بوتامیسول در شرایط تنش خشکی در سه سطح ظرفیت مزرعه (D1)، 50% (D2) و 75% تخلیه رطوبتی (D3) بودند. بالاترین مقادیر ارتفاع بوته (1.1±44.5 سانتی‌متر)، تعداد شاخه اصلی (1.5±26.4)، وزن بوته خشک (0.99±40.5 گرم در مترمربع)، کلروفیل کل (0.1±1.55 میلی‌گرم بر گرم وزن تر) و lpj,d اسانس (0.1±1.88%) توسط تیمار A2B2C2D1 در آویشن کوهی بدست آمد. تیمار A2B2C2D3 (استفاده از هر سه نوع کود با وجود تنش خشکی شدید) بیشترین محتوی پرولین (0.2±13.9 میکروگرم در گرم وزن تر) و کمترین میزان اسانس (0.1±0.79%) را در آویشن دنایی تولید کرد. ترکیب‌های غالب اسانس در گونه T. kotschyanus تیمول و کارواکرول، در گونه T. vulgaris، تیمول، گاما-ترپینن، پارا-سیمن و لینالول و در گونه T. daenensis، تیمول، کارواکرول، پارا-سیمن، بتا-کاریوفیلن، آلفا-پینن و میرسن بودند. بهره‌مندی بیشتر از مواد مغذی و تنش در حداقل ممکن به‌همراه آب و هوای معتدل منطقه مورد تحقیق، منجر به معرفی آویشن کوهی به‌عنوان گونه شاخص در این زمینه شد. | ||
| کلیدواژه‌ها | ||
| Thymus kotschyanus Boiss؛ اسانس؛ تیمول؛ کارواکرول | ||
| عنوان مقاله [English] | ||
| Effects of NPK complete fertilizer, botamisol, and humic acid on morphophysiological characteristics and essential oil in three Thymus species under drought stress conditions | ||
| نویسندگان [English] | ||
| M. Yadegari | ||
| Spicy and Aromatic Plants Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran | ||
| چکیده [English] | ||
| Due to the valuable role of thyme in the pharmaceutical and food industries of Iran, an experiment was conducted to study the effects of NPK complete fertilizers, butamisol, and humic acid on the morphophysiological characteristics and essential oil of three species of Thymus kotschyanus Boiss, T. vulgaris L., and T. daenensis Celark under drought stress conditions. This research was performed in three separate factorial experiments as a randomized complete block design with four replications in Islamic Azad University, Branch of Shahrekord in the 2021-2022 crop year. Foliar application was done twice with an interval of three weeks after the 10-leaf stage of the plant. Twenty-four experimental treatments included the non-use (A1) and use (A2) of NPK, non-use (B1) and use (B2) of humic acid, non-use (C1) and use (C2) of botamisol under drought stress conditions at three levels of field capacity (D1), 50% (D2), and 75% (D3) loss of gravitational water. The highest amounts of plant height (44.5±1.1 cm), number of main branches (26.4±1.5), plant dry weight (40.5±0.99 g.m-2), total chlorophyll (1.55±0.1 mg.g-1 fresh weight (FW)), and essential oil content (1.88±0.03%) were obtained in the A2B2C2D1 treatment in T. kotschyanus. The A2B2C2D3 treatment resulted in the highest proline content (13.9±0.2 µg.g-1 FW) and lowest essential oil content (0.79±0.1 %) in T. daenensis. Thymol and carvacrol in T. kotschyanus, thymol, ɣ-terpinene, p-cymene, and linalool in T. vulgaris, and thymol, carvacrol, p-cymene, β-caryophylene, α-pinene, and myrcene in T. daenensis were identified as the major essential oil compounds. More nutrient utilization and stress in the least possible along with the temperate climate of the study area led to the introduction of T. kotschyanus as an indicator species in this field. | ||
| کلیدواژه‌ها [English] | ||
| T. kotschyanus Boiss, essential oil, thymol, carvacrol | ||
| مراجع | ||
|
- Adams, R.P., 2007. Identification of Essential Oil Components by Gas Chromatography/ Mass Spectroscopy. Allured publishing Corp, Carol Stream, USA, 804p. - Albergaria, E.T., Oliveira, A.F. and Albuquerque, U.P., 2020. The effect of water deficit stress on the composition of phenolic compounds in medicinal plants. South African Journal of Botany, 131: 12-17. - Alizadeh, A., Najafi, F., Hadian, J. and Salehi, P., 2018. Effect of different levels of humic-acid and vermicompost extract on growth, yield, morphological and phytochemical properties of Satureja khuzistanica Jamzad. Journal of Agroecology, 10(1): 69-80. - Al Maqtari, M.A., Alghalibi, S.M. and Alhamzy, E.H., 2011. Chemical composition and antimicrobial activity of essential oil of Thymus vulgaris from Yemen. Turkish Journal of Biochemistry, 36(4): 342-349. - Amiri, H., 2012. Essential oils composition and antioxidant properties of three Thymus species. Evidence-Based Complementary and Alternative Medicine, 12: 8. - Arnon, D.I., 1975. Physiological principles of dry land crop production: 3-14. In: Gupta, U.S., (Ed.) Physiological Aspects of Dry Land Farming. Oxford Press, 391p. - Asadi, M., Nasiri, Y. and Morshedloo, M., 2018. Evaluation of quantitative and qualitative yield of Mentha piperita under amino acids, organic and chemical fertilizers. Suatainable Agriculture, 82(3): 257-275. - Asgarian, H., Abdossi, V., Danaee, E. and Ladan Moghadam, A., 2021. Effects of using humic acid and selenium on some morphophysiological characteristics of Calendula officinalis L. under salinity stress. Iranian Journal of Medicinal and Aromatic Plants Research, 37(4): 596-611. - Askary, M., Behdani, M.A., Parsa, S., Mahmoodi, S. and Jamialahmadi, M., 2018. Water stress and manure application affect the quantity and quality of essential oil of Thymus daenensis and Thymus vulgaris. Industrial Crops and Products, 111: 336-344. - Babaei, Kh., Moghaddam, M. and Farhadi, N., 2021. Morphological, physiological and phytochemical responses of Mexican marigold (Tagetes minuta L.) to drought stress. Scientia Horticulturae, 284: 110-116. - Barker, A.V. and Pilbeam D.J., 2007. Handbook of Plant Nutrition. CRC Press, 632p. - Bates, L.S., Waldren, R.P. and Teare, I.D., 1973. Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1): 205-207. - Bruneton, J., 1999. Pharmacognosy, Phytochemistry Medicinal Plants. Lavoisier Intercept, London, UK, 344p. - Caser, M., Chitarra, W., Angiolillod, F. and Perrone, I., 2019. Drought stress adaptation modulates plant secondary metabolite production in Salvia dolomitica Codd. Industrial Crops and Products, 129: 85-96. - Davarpanah, S., Tehranifar, A., Davarynejad, G.H. and Abadía, J., 2018. Effect of humic acid on some physical and chemical characteristics of Pomegranate (Punica granatum cv. Ardestani). Plant Production Technology, 10(1): 69-81. - Dere, S., Güneş, T. and Sivaci, R., 1998. Spectrophotometric determination of chlorophyll-A, B and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany, 22(1): 13-18. - Doskočil, L., Szewieczková, J.B., Enev, V., Kalina, L. and Wasserbauer, J., 2018. Spectral characterization and comparison of humic acids isolated from some European lignites. Fuel, 213: 123-132. - Elzaawely, A., Xuan, T. and Tawata, S., 2007. Changes in essential oil, kava pyrones and total phenolics of Alpinia zerumbet (Pers.) BL Burtt. and RM Sm. leaves exposed to copper sulphate. Environmental and Experimental Botany, 59: - Eman, E., Aziz, S.T. and Hendawi, E., 2008. Effect of soil type and irrigation intervals on plant growth, essential oil yield and constituents of Thymus vulgaris L. Ameriacan Eurasian Journal of Agricultural and Environmental Sciences, 4(4): 443-450. - Esch, E.H., Lipson, D.A. and Cleland, E.E., 2019. Invasion and drought alter phenological sensitivity and synergistically lower ecosystem production. Ecology, 100(10): 34-45. - Faten, S.A., Shaheen, A.M., Ahmad, A.A. and Mahmoud, A.R., 2010. Effect of foliar application of amino acids as antioxidants on growth, yield and characteristics of squash. Research Journal of Agriculture and Biological Science, 6(5): 583-588. - Fariaszewska, A., Aper, J., Van Huylenbroeck, J. and De Swaef, T., 2020. Physiological and biochemical responses of forage grass varieties to mild drought stress under field conditions. International Journal of Plant Production, 14: 335-353. - Ghanbarzadeh, Z., Mohsenzadeh, S., Rowshan, V. and Moradshahi, A., 2019. Evaluation of the growth, essential oil composition and antioxidant activity of Dracocephalum moldavica under water deficit stress and symbiosis with Claroideoglomus etunicatum and Micrococcus yunnanensis. Scientia Horticulturae, 256: 108652. - Gustafson, A.F., 2010. Handbook of Fertilizers-Their Sources, Make-Up, Effects and Use. Orange Judd Publishing Co., 180p. - Hasani, S.M., Azadfar, D., Arzanesh, M.H., Saeedi, Z. and Matinkhah, S.H., 2021. Effects of fertilizer treatments on morphological and qualitative traits of Descurainia sophia (L.) Schur and Coriandrum sativum L. in agroforestry systems. Iranian Journal of Medicinal and Aromatic Plants Research, 37(5): 766-780. - Hayati, A., Rahimi, M.M., Kelidari, A. and Hosseini, S.M., 2021. Effects of humic acid and iron nanochelate on osmolytes content of black cumin (Nigella sativa L.) under drought stress conditions. Iranian Journal of Medicinal and Aromatic Plants Research, 37(5): 809-821. - Juarez, C.R., Craker, L.E. and Rodríguez Mendoza, M.D., 2011. Humic substance and moisture content in the production of biomass and bioactive contituents of Thymus vulgaris L. Revista Fitotecnia Mexicana, 34(3): 183-188. - Khoshsokhan, F., Babalar, M., Chaghazardi, H.R. and Fatahi, M.R., 2012. Effect of salinity and drought stress on germination indices of two Thymus species. Cercetări Agronomice în Moldova, 1(149): 27-35. - Kulak, M., 2020. Recurrent drought stress effects on essential oil profile of Lamiaceae plants: An approach regarding stress memory. Industrial Crops and Products, 154: 1-17. - Lebaschi, M.H., Sharifi Ashorabadi, E., Makizadeh, M. and Talebpour, A.H., 2016. Evaluation of morphological characters, essential oil content/composition of Thymus kotschyanus Boiss. in varius densities undert drought stress. Journal of Ecophytochemistry of Medicinal Plants, 16(4): 37-48. - Mazooji, A., Salimpour, F., Danaei, M. and Akhoondi Darzikolaei, S., 2012. Comparative study of the essential oil chemical composition of Thymus kotschyanus Boiss. and Hohen var. kotschyanus from Iran. Annals of Biological Research, 3(3): 1443-1451. - Mc-Garvey, D. and Croteau, R., 1995. Terpenoid metabolism. The Plant Cell, 7: 1015-1026. - Mohammadi, M., Sefidkon, F., Asadi-Sanam, S. and Kalatejari, S., 2021. Effects of nutritional treatments on morphological characteristics and essential oil yield of Satureja khuzistanica Jamzad. Iranian Journal of Medicinal and Aromatic Plants Research, 37(2): 193-213.
- Mumivand, H., Ebrahimi, A., Morshedloo, M.R. and Shayganfar, A., 2021. Water deficit stress changes in drug yield, antioxidant enzymes activity and essential oil quality and quantity of Tarragon (Artemisia dracunculus L.). Industrial Crops and Products, 164: 113381. - Najafi vafa, Z., Sirousmehr, A. and Bijhani, M., 2020. Effect of different levels of humic acid and nano-zinc fertilizer on the antioxidant enzyme activities and essential oil of Savory (Satureja hortensis L.). Horticultural Plants Nutrition, 3(2): 43-58. - Nasiri, Y., Shekari, F. and Asadi, M., 2020. Effects of biofertilizers and zinc sulfate on some morphological and yield characteristics of Satureja hortensis L. Iranian Journal of Medicinal and Aromatic Plants Research, 36(4): 523-541. - Noori Hoseiny, S.M. and Zabihi, H., 2006. Effects of several fertilizers resourses and humic acid on morphological characters, yield and antioxidant of Bunium persicum Boiss. Journal of Applied Research, 29(4): 87-94. - Ozcan, M. and Chalchat, J.C., 2004. Aroma profile of Thymus vulgaris L. growing wild in Turkey. Bulgarian Journal of Plant Physiology, 30(3-4): 68-73. - Pourali, S. and Roozbahani, A., 2016. Effect of iron containing fertilizers and botamisol on some traits of Potato (Solanum tuberosum L.). Journal of Crop Ecophysiology, 10(1): 57-72. - Rezakhani, A. and Haj Seyed Hadi, M.R., 2016. Effect of manure and foliar application of amino acids on growth characteristics, seed yield and essential oil of coriander (Coriandrum sativum L.). Iranian Journal of Field Crop Science, 48(3): 777-786. - Sajjadi, S.E., Naderi, Gh., Ziaii, R. and Zolfaghari, B., 2004. The antioxidant activity of polyphenolic fraction of Thymus daenensis Celak. Iranian Journal of Pharmaceutical Research, 2: 80-81. - Sasani, N., Pâques, L.E., Boulanger, G. and Singh, A.P., 2021. Physiological and anatomical responses to drought stress differ between two larch species and their hybrid. Trees, 35: 1467-1484. - Sharafzadeh, Sh., 2011. Effect of nitrogen, phosphorous and potassium on growth, essential oil and total phenolic content of garden Thyme (Thymus vulgaris L.). Advances in Environmental Biology, 5(4): 699-703. - Tamburino, R., Vitale, M., Ruggiero, A., Sassi, M. and Sannino, L., 2017. Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biology, 17: 1-14. - Teimouri, M., 2012. Antimicrobial activity and essential oil composition of Thymus daenensis Celak from Iran. Journal of Medicinal Plants Research, 6(4): 631-635. - Thomas, J., Mandal, A.K.A., Raj Kumar, R. and Chrodia, A., 2009. Role of biologically active amino acid formulations on quality and crop productivity of Tea Camelia. International Journal of Agricultural Research, 4: 228-236. - Xiaolu, W., Jie, Y., Aoxue, L. and Yu, Ch., 2016. Drought stress and re-watering increase secondary metabolites andenzyme activity in dendrobium moniliforme. Industrial Crops and Products, 94: 385-393. - Yadegari, M., 2022. Effects of NPK, botamisol, and humic acid on morphophysiological traits and essential oil of three Satureja species under drought stress. Iranian Journal of Medicinal and Aromatic Plants Research, 38(1): 61-80. - Yadegari, M., 2017. Irrigation periods and Fe, Zn foliar application on agronomic characters of Borago officinalis, Calendula officinalis, Thymus vulgaris and Alyssum desertorum. Communication in Soil Science and Plant Analysis, 48(3): 307-315. - Yazdanpanah, S., Baghizadeh, A. and Abbassi, F., 2011. The interaction between drought stress and salicylic and ascorbic acids on some biochemical characteristics of Thymus hortensis. African Journal of Agricultural Research, 6(4): 798-807. - Zakerian, F., Sefidkon, F., Abbaszadeh, B. and Kalateh, S., 2020. Drought stress and micorrhiza fungi effects on physiologic and essential oil characters of Thymus sahandica Bornm. Iranian Journal of Horticultural Science, 51(1): 189-201. - Zandalinas, S.I., Mittler, R., Balfagon, D., Arbona, V. and Gomez-Cadenas, A., 2017. Plant adaptations to the combination of drought and high temperatures. Physiology of Plant, 162(1): 2-12. | ||
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