1Department of Medical Laboratory Techniques, College of Health and Medical Technology , Middle Technical University , Iraq
2Department of Anesthesia technique, Institute of Medical Technology / Baghdad, Middle Technical University, Iraq.
3Department of Medical Laboratory Techniques, College of Health and Medical Technology , Middle Technical University , Iraq .
چکیده
Proteus mirabilis belong to family Enterobacteriacea and capable to transform in shape from rod to elongated and swarming motility by flagella. It is opportunity bacteria and can cause different clinical diseases. Therefore, firstly this study aimed to assay and detect sequence of genes that encode for antibiotic resistance in multidrug resistance clinical isolates of Proteus mirabilis including blaTEM , aac(6’)-Ib ,qnrA, IntI2, IntI1 and secondly to investigate the relationship in phylogenetic tree among these genes in Iraq comparison with global strains in NCBI. The study included identification 500 clinical samples depending on morphological and biochemical tests and confirmation of Proteus mirabilis diagnosis by VITEK-2 Compact system. The confirmed isolates of Proteus mirabilis were 95 clinical isolates (19%). Antibiotic susceptibility test of all these isolates was done by using twelve antibiotics tested by using Amoxicillin, Aztreonam, Imipenem, Cefoxitin, Amikacin, Ceftazidem, Ciprofloxacin, Nalidixic acid, Gentamicin, Sulphamethazol-trimethoprim, Cefotaxim, Amoxicillin-calavulanic acid. The results showed that multidrug resistance Proteus mirabilis isolates contained the genes in different levels as follow blaTEM gene (90%), aac(6’)-Ib gene (80%) ,IntI1 gene (100%) ,IntI2 gene (80%). Sequencing of these genes carried out and detected phylogenetic relationship among these genes and global genes that documented in NCBI. The results showed that some Iraqi isolates contain genetic variation comparison with global strains, so, this variation detected and registered in NCBI of all five antibiotic resistance genes mention above and accepted under accession numbers of aacIb gene (LC613168.1), blaTEM gene (LC613166.1) , IntI1 gene (LC613169.1 ) , IntI2 gene ( LC613170.1) .