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Abed Gumar, E, Salim Hamzah, A, Fadhil Hamad, W. (1401). Study of Some Resistance Genes in Clinical Proteus mirabilis. سامانه مدیریت نشریات علمی, 77(6), 2235-2242. doi: 10.22092/ari.2022.358489.2230
E Abed Gumar; A Salim Hamzah; W Fadhil Hamad. "Study of Some Resistance Genes in Clinical Proteus mirabilis". سامانه مدیریت نشریات علمی, 77, 6, 1401, 2235-2242. doi: 10.22092/ari.2022.358489.2230
Abed Gumar, E, Salim Hamzah, A, Fadhil Hamad, W. (1401). 'Study of Some Resistance Genes in Clinical Proteus mirabilis', سامانه مدیریت نشریات علمی, 77(6), pp. 2235-2242. doi: 10.22092/ari.2022.358489.2230
Abed Gumar, E, Salim Hamzah, A, Fadhil Hamad, W. Study of Some Resistance Genes in Clinical Proteus mirabilis. سامانه مدیریت نشریات علمی, 1401; 77(6): 2235-2242. doi: 10.22092/ari.2022.358489.2230

Study of Some Resistance Genes in Clinical Proteus mirabilis

Archives of Razi Institute
مقاله 27، دوره 77، شماره 6، بهمن و اسفند 2022، صفحه 2235-2242    اصل مقاله (728.05 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358489.2230
نویسندگان
E Abed Gumar1؛ A Salim Hamzah* 2؛ W Fadhil Hamad1
1Department of Medical Laboratory Techniques, College of Health and Medical Technology, Middle Technical University, Baghdad, Iraq
2Department of Anesthesia technique, Institute of Medical Technology, Middle Technical University, Baghdad, Iraq
چکیده
Proteus mirabilis belongs to the family Enterobacteriaceae and is capable of transforming in shape from rod to elongated and swarming motility by flagella. It is an opportunity for bacteria and can cause different clinical diseases. Therefore, this study aimed to assay and detect a sequence of genes that encode for antibiotic resistance in multidrug resistance clinical isolates of Proteus mirabilis, including blaTEM, aac(6’)-Ib, qnrA, IntI2, IntI1 and secondly to investigate the relationship in the phylogenetic tree among these genes in Iraq comparison with global strains in NCBI. The study included the identifying of 500 clinical samples depending on morphological and biochemical tests and confirming Proteus mirabilis diagnosis by the VITEK-2 Compact system. The confirmed isolates of Proteus mirabilis were 95 clinical isolates (19%). Antibiotic susceptibility test of all these isolates was done using twelve antibiotics tested using Amoxicillin, Aztreonam, Imipenem, Cefoxitin, Amikacin, Ceftazidem, Ciprofloxacin, Nalidixic acid, Gentamicin, Sulphamethazol-trimethoprim, Cefotaxime, Amoxicillin-clavulanic acid. The results showed that multidrug resistance Proteus mirabilis isolates contained the genes in different levels as follow blaTEM gene (90%), aac(6’)-Ib  gene (80%) ,IntI1 gene (100%), IntI2 gene (80%). These genes were sequenced and detected phylogenetic relationships among these genes and global genes were documented in NCBI. The results showed that some Iraqi isolates contain genetic variation compared to global strains. Therefore, this variation was detected and registered in NCBI of all five antibiotic resistance genes mentioned above and accepted under accession numbers of aacIb gene (LC613168.1), blaTEM gene (LC613166.1), IntI1 gene (LC613169.1), IntI2 gene (LC613170.1).
کلیدواژه‌ها
Multidrug Proteus mirabilis؛ Antibiotic resistance genes؛ Integrons؛ Iraqi clinical samples؛ NCBI
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