اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,034
تعداد مقالات33,949
تعداد مشاهده مقاله45,412,689
تعداد دریافت فایل اصل مقاله68,717,295
Abed Gumar, E, Salim Hamzah, A, Fadhil Hamad, W. (1401). Study of Some Resistance Genes in Clinical Proteus mirabilis. سامانه مدیریت نشریات علمی, 77(6), 2235-2242. doi: 10.22092/ari.2022.358489.2230
E Abed Gumar; A Salim Hamzah; W Fadhil Hamad. "Study of Some Resistance Genes in Clinical Proteus mirabilis". سامانه مدیریت نشریات علمی, 77, 6, 1401, 2235-2242. doi: 10.22092/ari.2022.358489.2230
Abed Gumar, E, Salim Hamzah, A, Fadhil Hamad, W. (1401). 'Study of Some Resistance Genes in Clinical Proteus mirabilis', سامانه مدیریت نشریات علمی, 77(6), pp. 2235-2242. doi: 10.22092/ari.2022.358489.2230
Abed Gumar, E, Salim Hamzah, A, Fadhil Hamad, W. Study of Some Resistance Genes in Clinical Proteus mirabilis. سامانه مدیریت نشریات علمی, 1401; 77(6): 2235-2242. doi: 10.22092/ari.2022.358489.2230

Study of Some Resistance Genes in Clinical Proteus mirabilis

Archives of Razi Institute
مقاله 27، دوره 77، شماره 6، بهمن و اسفند 2022، صفحه 2235-2242    اصل مقاله (728.05 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358489.2230
نویسندگان
E Abed Gumar1؛ A Salim Hamzah* 2؛ W Fadhil Hamad1
1Department of Medical Laboratory Techniques, College of Health and Medical Technology, Middle Technical University, Baghdad, Iraq
2Department of Anesthesia technique, Institute of Medical Technology, Middle Technical University, Baghdad, Iraq
چکیده
Proteus mirabilis belongs to the family Enterobacteriaceae and is capable of transforming in shape from rod to elongated and swarming motility by flagella. It is an opportunity for bacteria and can cause different clinical diseases. Therefore, this study aimed to assay and detect a sequence of genes that encode for antibiotic resistance in multidrug resistance clinical isolates of Proteus mirabilis, including blaTEM, aac(6’)-Ib, qnrA, IntI2, IntI1 and secondly to investigate the relationship in the phylogenetic tree among these genes in Iraq comparison with global strains in NCBI. The study included the identifying of 500 clinical samples depending on morphological and biochemical tests and confirming Proteus mirabilis diagnosis by the VITEK-2 Compact system. The confirmed isolates of Proteus mirabilis were 95 clinical isolates (19%). Antibiotic susceptibility test of all these isolates was done using twelve antibiotics tested using Amoxicillin, Aztreonam, Imipenem, Cefoxitin, Amikacin, Ceftazidem, Ciprofloxacin, Nalidixic acid, Gentamicin, Sulphamethazol-trimethoprim, Cefotaxime, Amoxicillin-clavulanic acid. The results showed that multidrug resistance Proteus mirabilis isolates contained the genes in different levels as follow blaTEM gene (90%), aac(6’)-Ib  gene (80%) ,IntI1 gene (100%), IntI2 gene (80%). These genes were sequenced and detected phylogenetic relationships among these genes and global genes were documented in NCBI. The results showed that some Iraqi isolates contain genetic variation compared to global strains. Therefore, this variation was detected and registered in NCBI of all five antibiotic resistance genes mentioned above and accepted under accession numbers of aacIb gene (LC613168.1), blaTEM gene (LC613166.1), IntI1 gene (LC613169.1), IntI2 gene (LC613170.1).
کلیدواژه‌ها
Multidrug Proteus mirabilis؛ Antibiotic resistance genes؛ Integrons؛ Iraqi clinical samples؛ NCBI
سایر فایل های مرتبط با مقاله
مراجع
  1. Adamus-Bialek W, Zajac E, Parniewski P, Kaca W. Comparison of antibiotic resistance patterns in collections of Escherichia coli and Proteus mirabilis uropathogenic strains. Mol Biol Rep. 2013;40(4):3429-35.
  2. Schaffer JN, Pearson MM. Proteus mirabilis and Urinary Tract Infections. Microbiol Spectr. 2015;3(5).
  3. Slattery S, Tony Pembroke J, Murnane JG, Ryan MP. Isolation, nucleotide sequencing and genomic comparison of a Novel SXT/R391 ICE mobile genetic element isolated from a municipal wastewater environment. Sci Rep. 2020;10(1):8716.
  1. Bitar I, Mattioni Marchetti V, Mercato A, Nucleo E, Anesi A, Bracco S, et al. Complete Genome and Plasmids Sequences of a Clinical Proteus mirabilis Isolate Producing Plasmid Mediated NDM-1 from Italy. Microorganisms. 2020;8(3).
  2. Mac Aogain M, Rogers TR, Crowley B. Identification of emergent bla CMY-2 -carrying Proteus mirabilis lineages by whole-genome sequencing. New Microbes New Infect. 2016;9:58-62.
  3. Sun L, Xu J, He F. Genomic characterisation of a Proteus mirabilis clinical isolate from China carrying blaNDM-5 on an IncX3 plasmid. J Glob Antimicrob Resist. 2019;19:317-9.
  4. Kanzari L, Ferjani S, Saidani M, Hamzaoui Z, Jendoubi A, Harbaoui S, et al. First report of extensively-drug-resistant Proteus mirabilis isolate carrying plasmid-mediated blaNDM-1 in a Tunisian intensive care unit. Int J Antimicrob Agents. 2018;52(6):906-9.
  5. Firmo EF, Beltrao EMB, Silva F, Alves LC, Brayner FA, Veras DL, et al. Association of blaNDM-1 with blaKPC-2 and aminoglycoside-modifying enzyme genes among Klebsiella pneumoniae, Proteus mirabilis and Serratia marcescens clinical isolates in Brazil. J Glob Antimicrob Resist. 2020;21:255-61.
  6. Valentin T, Feierl G, Masoud-Landgraf L, Kohek P, Luxner J, Zarfel G. Proteus mirabilis harboring carbapenemase NDM-5 and ESBL VEB-6 detected in Austria. Diagn Microbiol Infect Dis. 2018;91(3):284-6.
  7. CLSI. Performance standard for antimicrobial susceptibility testing, Twenty-Fourth Informational Supplement: Clinical and Laboratory Standards Institute. 2019.
  8. Kim HB, Park CH, Kim CJ, Kim EC, Jacoby GA, Hooper DC. Prevalence of plasmid-mediated quinolone resistance determinants over a 9-year period. Antimicrob Agents Chemother. 2009;53(2):639-45.
  9. Belaaouaj A, Lapoumeroulie C, Canica MM, Vedel G, Nevot P, Krishnamoorthy R, et al. Nucleotide sequences of the genes coding for the TEM-like beta-lactamases IRT-1 and IRT-2 (formerly called TRI-1 and TRI-2). FEMS Microbiol Lett. 1994;120(1-2):75-80.
  10. Dillon B, Thomas L, Mohmand G, Zelynski A, Iredell J. Multiplex PCR for screening of integrons in bacterial lysates. J Microbiol Methods. 2005;62(2):221-32.
  11. Coker C, Poore CA, Li X, Mobley HLT. Pathogenesis of Proteus mirabilisurinary tract infection. Microbes Infect. 2000;2(12):1497-505.
  12. O'Hara CM, Brenner FW, Miller JM. Classification, identification, and clinical significance of Proteus, Providencia, and Morganella. Clin Microbiol Rev. 2000;13(4):534-46.
  13. Chikere C, Omoni V, Chikere B. Distribution of Potential Nosocomial Pathogens in an Hospital Environment. Afr J Biotechnol. 2008;7:3535-9.
  14. Okesola AO, Makanjuola O. Resistance to Third-Generation Cephalosporins and Other Antibiotics by Enterobacteriaceae in Western Nigeria. Am J Infect Dis. 2009;5(1).
  15. Soge OO, Adeniyi BA, Roberts MC. New antibiotic resistance genes associated with CTX-M plasmids from uropathogenic Nigerian Klebsiella pneumoniae. J Antimicrob Chemother. 2006;58(5):1048-53.
  16. Ogbolu DO, Daini OA, Ogunledun A, Alli AO, Webber MA. High levels of multidrug resistance in clinical isolates of Gram-negative pathogens from Nigeria. Int J Antimicrob Agents. 2011;37(1):62-6.
  17. Paterson DL, Bonomo RA. Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev. 2005;18(4):657-86.
  18. Canton R, Gonzalez-Alba JM, Galan JC. CTX-M Enzymes: Origin and Diffusion. Front Microbiol. 2012;3:110.
  19. Tijjani J, Arzai A, Sadiq N. Antimicrobial susceptibility pattern of extended-spectrum beta-lactamase producers in Gram negative urogenital isolates in Kano, Nigeria. Bayero J Pure Appl Sci. 2012;5:20-5.
  20. EnabuleleI O, YahS C, YusufE. O, EghafonaN. O, editors. Emerging quinolones resistant transfer genes among gram-negative bacteria, isolated from faeces of HIV/AIDS patients attending some Clinics and Hospitals in the City of Benin, Edo State, Nigeria. 2006.
  21. Martínez-Martínez L, Pascual A, Jacoby GA. Quinolone resistance from a transferable plasmid. Lancet (London, England). 1998;351(9105):797-9.
  22. Chen X, Zhang W, Pan W, Yin J, Pan Z, Gao S, et al. Prevalence of qnr, aac(6')-Ib-cr, qepA, and oqxAB in Escherichia coli isolates from humans, animals, and the environment. Antimicrob Agents Chemother. 2012;56(6):3423-7.
  1. Tran JH, Jacoby GA. Mechanism of plasmid-mediated quinolone resistance. Proc Natl Acad Sci U S A. 2002;99(8):5638-42.
  2. Tran JH, Jacoby GA, Hooper DC. Interaction of the plasmid-encoded quinolone resistance protein Qnr with Escherichia coli DNA gyrase. Antimicrob Agents Chemother. 2005;49(1):118-25.
  3. Jacoby GA. Mechanisms of resistance to quinolones. Clin Infect Dis. 2005;41 Suppl 2:S120-6.
  4. Robicsek A, Jacoby GA, Hooper DC. The worldwide emergence of plasmid-mediated quinolone resistance. Lancet Infect Dis. 2006;6(10):629-40.
  5. Olowe O, Oladipo G, Makanjuola O, Olaitan J. Prevalence of extended-spectrum beta-lactamases (ESBLs)carrying genes in Klebsiella spp. from clinical samples at Ile-Ife, South Western Nigeria. Int J Pharma Med Biol Sci. 2012;1:2278-5221.
  6. Perichon B, Courvalin P, Galimand M. Transferable resistance to aminoglycosides by methylation of G1405 in 16S rRNA and to hydrophilic fluoroquinolones by QepA-mediated efflux in Escherichia coli. Antimicrob Agents Chemother. 2007;51(7):2464-9.
  7. Yamane K, Wachino J, Suzuki S, Kimura K, Shibata N, Kato H, et al. New plasmid-mediated fluoroquinolone efflux pump, QepA, found in an Escherichia coli clinical isolate. Antimicrob Agents Chemother. 2007;51(9):3354-60.
  8. Boucher Y, Labbate M, Koenig JE, Stokes HW. Integrons: mobilizable platforms that promote genetic diversity in bacteria. Trends Microbiol. 2007;15(7):301-9.
  9. Song W, Kim J, Bae IK, Jeong SH, Seo YH, Shin JH, et al. Chromosome-encoded AmpC and CTX-M extended-spectrum beta-lactamases in clinical isolates of Proteus mirabilis from Korea. Antimicrob Agents Chemother. 2011;55(4):1414-9.
  10. Alabi OS, Mendonca N, Adeleke OE, da Silva GJ. Molecular screening of antibiotic-resistant determinants among multidrug-resistant clinical isolates of Proteus mirabilis from SouthWest Nigeria. Afr Health Sci. 2017;17(2):356-65.
آمار
تعداد مشاهده مقاله: 15,332
تعداد دریافت فایل اصل مقاله: 48,617


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب