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Didarkhah, M, Vatandoost, M. (1401). Comparison of the effect of Gonadotropin-Releasing Hormone and Human Chorionic Gonadotropin on Reproductive Performance of Romanov Ewes During the Natural Breeding Season. سامانه مدیریت نشریات علمی, 77(3), 991-998. doi: 10.22092/ari.2021.354332.1633
M Didarkhah; M Vatandoost. "Comparison of the effect of Gonadotropin-Releasing Hormone and Human Chorionic Gonadotropin on Reproductive Performance of Romanov Ewes During the Natural Breeding Season". سامانه مدیریت نشریات علمی, 77, 3, 1401, 991-998. doi: 10.22092/ari.2021.354332.1633
Didarkhah, M, Vatandoost, M. (1401). 'Comparison of the effect of Gonadotropin-Releasing Hormone and Human Chorionic Gonadotropin on Reproductive Performance of Romanov Ewes During the Natural Breeding Season', سامانه مدیریت نشریات علمی, 77(3), pp. 991-998. doi: 10.22092/ari.2021.354332.1633
Didarkhah, M, Vatandoost, M. Comparison of the effect of Gonadotropin-Releasing Hormone and Human Chorionic Gonadotropin on Reproductive Performance of Romanov Ewes During the Natural Breeding Season. سامانه مدیریت نشریات علمی, 1401; 77(3): 991-998. doi: 10.22092/ari.2021.354332.1633

Comparison of the effect of Gonadotropin-Releasing Hormone and Human Chorionic Gonadotropin on Reproductive Performance of Romanov Ewes During the Natural Breeding Season

Archives of Razi Institute
مقاله 9، دوره 77، شماره 3، مرداد و شهریور 2022، صفحه 991-998    اصل مقاله (392.69 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.354332.1633
نویسندگان
M Didarkhah* 1؛ M Vatandoost2
1Sarayan Faculty of Agriculture, University of Birjand, Birjand, Iran
2Department of Agriculture, Payame Noor University, POX19395-3697, Tehran, Iran
چکیده
Low pregnancy rate is an important issue in small ruminants. Superovulation protocols would serve as the possible techniques to increase the pregnancy rate in small ruminants due to the use of synthetic hormones. The present study aimed to assess the effect of gonadotropin-releasing hormone (GnRH) and Human chorionic gonadotropin (hCG) after artificial insemination (AI) on the pregnancy rate of Romanov ewes breed. To this end, estrus was synchronized in 140 ewes using a controlled internal drug-releasing (CIDR) device. Following CIDR removal, all ewes received 400 IU Pregnant Mare's Serum Gonadotropin (PMSG). The ewes were assigned to three groups (control, n=20; experimental groups, each of them was divided into three subgroups, n=20). The first group in each experiment was assumed as control. The second and third groups received three levels of GnRH, (400, 500, and 600 µg, n=20) and hCG (200, 300, and 400 IU) administered by the intramuscular (IM) route. As soon as the signs of estrus were observed, the ewes were isolated from the others and artificially inseminated with fresh sperm using the transcervical method. The results indicated that there were no significant differences between experimental groups in ovulation and pregnancy rates on days 33 and 90 post-AI (P>0.05). The highest twin birth rate (20%) was recorded in the hCG group (300 IU), as compared to GnRH and control groups (P<0.05). The effect of GnRH and hCG on the plasma concentration of progesterone was significant (P<0.01). The groups significantly differed in fecundity rate (Single birth, twin birth, and triple birth) (P<0.05), all of these parameters were significantly higher in the hCG 300 IU group. It can be concluded that the injection of 300 IU of hCG hormone after insemination improves pregnancy rate in ewes.
کلیدواژه‌ها
Artificial insemination؛ CIDR؛ GnRH؛ hCG
عنوان مقاله [English]
مقایسه استفاده از هورمون های hCG و GnRH بر عملکرد تولید مثلی میش های رومانف در فصل تولیدمثل
چکیده [English]
پایین بودن نرخ آبستنی مسئله مهمی در نشخوارکنندگان کوچک است. یکی از تکنیک های سوپراولاسیون جهت افزایش میزان نرخ آبستنی در نشخوارکتتدگان استفاده از هورمون های مصنوعی می باشد. هدف از این مطالعه بررسی تأثیر GnRH و hCG پس از تلقیح مصنوعی بر میزان نرخ آبستنی میش های نژاد رومانوف بود. برای این منظور ، تعداد 140 راس میش با استفاده از CIDR همزمان سازی فحلی شدند. پس از خروج CIDR ، همه میش ها 400 واحد بین المللی PMSG دریافت کردند. میش ها به 3 گروه تقسیم شدند (شاهد ، 20 راس ، گروه های آزمایش ، 60 راس). گروه اول به عنوان شاهد در نظر گرفته شد. گروه های دوم و سوم 3 سطح GnRH ، (400 ، 500 و 600 میکروگرم) و hCG (200 ، 300 و 400 واحد بین المللی) از طریق تزریق عضلانی دریافت کردند. . به محض مشاهده علائم فحلی ، میشهای فحل جدا شده و با استفاده از روش ترانس سرویکال با اسپرم تازه تلقیح مصنوعی شدند. نتایج نشان داد که بین روزهای 33 و 90 پس از تلقیح مصنوعی بین گروههای آزمایش از نظر میزان تخمک گذاری و میزان آبستنی تفاوت معنی داری وجود ندارد (P> 0.05). بیشترین نرخ دوقلوزایی (20 درصد) در گروه hCG (300 واحد بین المللی) در مقایسه با GnRH و گروه کنترل مشاهده شد (05/0> P). اثر GnRH و hCG بر غلظت پروژسترون در پلاسما معنی دار بود (01 / 0 > P). میزان نرخ باروری (تک قلو ، دوقلو و سه قلو) بین گروه ها تفاوت معنی داری داشت (05/0> P). می توان نتیجه گرفت که تزریق 300 واحد بین المللی هورمون hCG پس از تلقیح باعث بهبود میزان آبستنی در میش ها می شود.
کلیدواژه‌ها [English]
تلقیح مصنوعی, گونادوتروپین, همزمان سازی فحلی
مراجع
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