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Mansor, M. R, Ali Hasan Kashkool, D, Safaa Abd Al-Ameer, S, Akeel Al-Hasan, B, Almulla, A. F. (1401). Molecular Detection of blaSHV-la Gene in Klebsiella pneumonia Isolated from Urinary Tract Infections, Najaf, Iraq. سامانه مدیریت نشریات علمی, 77(3), 1181-1184. doi: 10.22092/ari.2022.357617.2070
M. R Mansor; D Ali Hasan Kashkool; S Safaa Abd Al-Ameer; B Akeel Al-Hasan; A. F Almulla. "Molecular Detection of blaSHV-la Gene in Klebsiella pneumonia Isolated from Urinary Tract Infections, Najaf, Iraq". سامانه مدیریت نشریات علمی, 77, 3, 1401, 1181-1184. doi: 10.22092/ari.2022.357617.2070
Mansor, M. R, Ali Hasan Kashkool, D, Safaa Abd Al-Ameer, S, Akeel Al-Hasan, B, Almulla, A. F. (1401). 'Molecular Detection of blaSHV-la Gene in Klebsiella pneumonia Isolated from Urinary Tract Infections, Najaf, Iraq', سامانه مدیریت نشریات علمی, 77(3), pp. 1181-1184. doi: 10.22092/ari.2022.357617.2070
Mansor, M. R, Ali Hasan Kashkool, D, Safaa Abd Al-Ameer, S, Akeel Al-Hasan, B, Almulla, A. F. Molecular Detection of blaSHV-la Gene in Klebsiella pneumonia Isolated from Urinary Tract Infections, Najaf, Iraq. سامانه مدیریت نشریات علمی, 1401; 77(3): 1181-1184. doi: 10.22092/ari.2022.357617.2070

Molecular Detection of blaSHV-la Gene in Klebsiella pneumonia Isolated from Urinary Tract Infections, Najaf, Iraq

Archives of Razi Institute
مقاله 32، دوره 77، شماره 3، مرداد و شهریور 2022، صفحه 1181-1184    اصل مقاله (254.18 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357617.2070
نویسندگان
M. R Mansor* 1؛ D Ali Hasan Kashkool2؛ S Safaa Abd Al-Ameer1؛ B Akeel Al-Hasan1؛ A. F Almulla1
1Department of Medical Laboratory Techniques, Faculty of Medical Technologies, Islamic University, Najaf, Iraq
2Directorate of Education, Ministry of Education, Najaf, Iraq
چکیده
Klebsiella pneumoniae is a gram-negative bacterium that causes serious illnesses, including pneumonia, liver abscess, meningitis, bloodstream infections, and urinary tract infections (UTIs). This study aimed to isolate and diagnose K. pneumoniae from clinical specimens of urine from patients with UTIs and perform molecular detection of the blaSHV-la gene in K. pneumonia in the Najaf Province, Iraq. The study included 100 clinical specimens from October 2021 to March 2022. As an initial diagnosis, K. pneumoniae isolates were diagnosed based on culture and biochemical features. Apart from the usage of polymerase chain reaction (PCR) technology to identify the blaSHV-la gene, the final diagnostic was achieved by the automated Vitek-2 compact system. The biochemical findings revealed that 40 out of every 100 isolates tested positive for K. pneumoniae. These results were validated by Vitek, which revealed that 40/100 of the samples tested positive for K. pneumoniae, and by PCR utilizing the blaSHV-la gene, which showed that 13/40 of the samples tested positive for K. pneumoniae isolated from the urine of patients with UTIs. In conclusion, the results indicated that the use of the Vitek-2 technique was required to confirm the accurate identification of the pathogen. Klebsiella pneumoniae clinical isolates showed multidrug resistance to antibiotics commonly used to treat UTIs. The blaSHV gene encoded for Extended-spectrum beta-lactam antibiotic was found almost in K. pneumoniae isolates.
کلیدواژه‌ها
Klebsiella pneumoniae؛ blaSHV-la gene؛ Urinary tract
مراجع
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