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بررسی ویژگی‌های موفولوژیک و بیوشیمیایی مرتبط با عمرگلجایی در جمعیت های نرگس شهلا (Narcissus tazetta L.) در شرایط آب و هوایی خوزستان | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 10، دوره 29، شماره 2 - شماره پیاپی 58، اسفند 1400، صفحه 282-296 اصل مقاله (820.25 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2022.357612.1407 | ||
| نویسندگان | ||
| فاطمه برفی1؛ محمدرضا صالحی سلمی* 2؛ احمد زارع3 | ||
| 1دانش‌آموخته کارشناسی ارشد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان | ||
| 2نویسنده مسئول، دانشیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان | ||
| 3استادیار، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان | ||
| چکیده | ||
| گل نرگس شهلا (Narcissus tazetta) از تیره Amaryllidaceae، ازنظر ویژگی های زینتی و دارویی اهمیت فراوانی در دنیا دارد. هدف از این پژوهش بررسی تنوع 13 جمعیت نرگس از مناطق معتدل و گرمسیری کشور برای یافتن جمعیت های برتر از نظر کیفیت پس از برداشت، با توجه به شاخص های مورفولوژیک و بیوشیمیایی بود. این پژوهش به صورت طرح بلوک های کامل تصادفی با 4 تکرار در مزرعه انجام شد. در این تحقیق، شاخص های مؤثر بر کیفیت گل بریده شامل: تعداد ساقه گل دهنده، طول ساقه، وزن ساقه همراه گل، جذب محلول، کیفیت ظاهری، کلروفیل برگ، پرولین گلچه، کربوهیدراتهای محلول گلچه، فنول کل گلچه، محتوای مالون دی آلدئید گلچه، پروتئین گلچه، فعالیت آنزیمهای پراکسیداز و کاتالاز گلچه اندازه گیری شد. نتایج تجزیه واریانس نشان داد که تفاوت بین جمعیت ها برای کلیه صفات بجز کیفیت ظاهری معنی دار بود. بر اساس تجزیه خوشهای داده های بیوشیمیایی، جمعیت ها در دو گروه اصلی تقسیم شدند. گروه اول شامل جمعیت های کازرون، بهبهان، جهرم، ایلام، شیراز و کرمان بود. در این گروه؛ صفات مهمی هم چون کلروفیل کل، جذب محلول و فنول کل بیشترین مقدار را داشتند. گروه دوم به دو زیر گروه تقسیم گردید. در زیر گروه الف جمعیت های خوسف، قائمشهر و خرم آباد قرار داشتند و در زیر گروه ب اهواز، آبدانان، گچساران و مهران قرار گرفتنتد. زیر گروه الف صفاتی چون محتوای پرولین، مالوندی آلدئید، آنزیمهای آنتی اکسیدانی پراکسیداز و کاتالاز عوامل مؤثر در این گروه بودند. در زیر گروه ب ویژگی های پروتئین کل و کربوهیدراتهای محلول بیشترین مقدار را داشتند. از لحاظ عمرگلجایی جمعیتهای اهواز، آبدانان، گچساران و مهران که بیشترین مقدار پروتئین کل و کربوهیدرات های محلول را در پایان آزمایش داشتند و محتوای پرولین، مالوندی آلدئید، آنزیم های آنتی اکسیدانی پراکسیداز و کاتالاز آنها پس از گذشت 10 روز در سطح پایینی بود به عنوان جمعیتهای برتر معرفی شدند. به طور کلی این ارزیابی نشان داد تنوع مورفولوژیک و بیوشیمیایی بالایی در جمعیتهای بومی نرگس وجود دارد که میتواند در نتیجه تنوع اقلیمی بسیار متفاوت در ایران و هم چنین جهش در این گیاه باشد. در مجموع درک چنین تنوع بالایی در مدیریت و حفاظت ژرمپلاسم این گیاه مفید می باشد و بهنژادگر را در تعیین راهبردهای بهرهبرداری، اصلاح و اهلی سازی و کشت و کار این گیاه یاری میکند. | ||
| کلیدواژه‌ها | ||
| آنتی‌اکسیدانتی؛ جذب محلول؛ دندروگرام؛ ژنوتیپ؛ عمرگلجای | ||
| عنوان مقاله [English] | ||
| Investigation of morphological and biochemical traits charactristics related to vase life in population Narcissus (Narcissus tazetta L.) in Khuzestan climate Iran | ||
| نویسندگان [English] | ||
| F. Barfi1؛ M.R. Salehi Salmi2؛ A. Zare3 | ||
| 1M.Sc., Department of Horticultural Science, Agricultural Sciences and Natural Resources University of Khuzestan, Iran. | ||
| 2Corresponding author, Assoc. Prof. Department of Horticultural Science, Agricultural Sciences and Natural Resources University of Khuzestan, Iran. | ||
| 3Assist. Prof. Plant Production and Genetics Department Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Iran. | ||
| چکیده [English] | ||
| Narcissus is a member of the Amaryllidaceae family. This specie is very important in the world as an ornamental plant. In order to study the diversity between narcissus genotypes, an experiment was conducted in a completely randomized block design with 13 genotypes and 4 replications at Agricultural Sciences and Natural Resources University of Khuzestan. In this study, effective indicators on cut flower quality include: number of flowers, stem height, stem and flowers weight, solution uptake, visual quality, chlorophyll of leaf, florets proline, soluble carbohydrates of florets, total phenols of florets, malondialdehyde content of florets, protein, activity of peroxidase and catalase enzymes activities were measured. The results showed that based on the cluster analysis of physiological data, genotypes were divided into two main groups. The first group included the genotypes of Kazerun, Behbahan, Jahrom, Ilam, Shiraz and Kerman. In this group, important traits such as total chlorophyll, solution absorption and total phenol had the highest amount. The second group was divided into two subgroups. Subgroup A included Khosf, Ghaemshahr and Khorramabad genotypes and subgroup B included Ahvaz, Abdanan, Gachsaran and Mehran. Subgroup A, traits such as proline content, malondia aldehyde, antioxidant enzymes peroxidase and catalase were effective factors in this group. Subgroup B had the highest total protein and soluble carbohydrate properties. It was found that the populations of Ahvaz, Abdanan, Gachsaran and Mehran, having the highest amount of total protein and soluble carbohydrates at the end of the experiment, and the content of proline, malondialdehyde, antioxidant enzymes peroxidase and catalase were at a low level, after 10 days, therefore, they introduced as superior populations for cut flowers. In general, the study showed that there is a high morphophysiological diversity in the native genus of narcissus, which can be the result of very different climatic diversity in Iran, as well as mutation and sexual reproduction by seeds in this plant. In general, understanding such a high diversity is useful in the management and protection of the germplasm of this plant and helps the breeder in determining the strategies of exploitation, breeding and domestication and cultivation of this plant. | ||
| کلیدواژه‌ها [English] | ||
| Antioxidant, dendrogram, genotype, solution absorption, Vase-life | ||
| مراجع | ||
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