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انگشت‌نگاری گونه‌های جنس آژیلوپس (Ageilops) با نشانگرهای مبتنی بر ژنهای هدفمند و نواحی حفاظت شده CoRAP | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 5، دوره 29، شماره 2 - شماره پیاپی 58، اسفند 1400، صفحه 207-220 اصل مقاله (1.04 M) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2021.354417.1382 | ||
| نویسندگان | ||
| صدیقه فابریکی اورنگ* 1؛ حمید کریمی2؛ جعفر احمدی3؛ علی اشرف مهرابی4 | ||
| 1دانشیار، گروه ژنتیک و به‌نژادی گیاهی، دانشگاه بین‌المللی امام خمینی، قزوین، ایران. | ||
| 2کارشناس ارشد ژنتیک و به‌نژادی گیاهی، دانشگاه بین‌المللی امام خمینی، قزوین، ایران | ||
| 3استاد، گروه ژنتیک و به‌نژادی گیاهی، دانشگاه بین‌المللی امام خمینی(ره)، قزوین، ایران | ||
| 4دانشیار پژوهش، بخش زیست فناوری موسسه تحقیقات جنگلها و مراتع، سازمان تحقیقات، آموزش و ترویج کشاورزی. تهران، ایران | ||
| چکیده | ||
| گونه های جنس آژیلوپس (Ageilops) از گرامینه های مرتعی و یکی از مهمترین اجداد گندم و منبع غنی از ژنهای تحمل به تنش هاست. بدین‌منظور انگشت‌نگاری روابط ژنتیکی توده های متعلق به هشت گونه Ageilopsبا استفاده از نشانگرهای مولکولی CoRAP مبتنی بر ژنهای هدفمند مورد مطالعه قرار گرفت. در طراحی آغازگرهای ثابت از شش ژن عامل تحمل تنش های غیرزیستی CAT، MnSoD، SoS1، miR398، miR160b و miR169gR استفاده شد. کمترین و بیشترین محتوای اطلاعات چندشکلی بهترتیب مربوط به آغازگرهای CoRAP2 و CoRAP10 با مقادیر 92/0 و 96/0 بود. شاخص نشانگری در بین نشانگرهای مورد بررسی از 89/6 در آغازگر CoRAP7 تا 49/13 در آغازگر CoRAP9 متغیر بود. بین گونه ها جریان ژنی (Nm) پایین و بعکس میزان تمایز (Gst) بالایی مشاهده شد. همچنین مقدار شاخص Fst برابر با 45/0 نشان داد که جمعیت های مورد مطالعه کاملاً از هم متمایز شده اند. با توجه به بالا بودن شاخصهای تعداد آلل مؤثر و تنوع ژنتیکی Nei، دو گونه Ae. cylandrica و Ae. caudata تنوع درون گونه ای بالایی در بین گونه های مورد بررسی نشان دادند. بیشترین تشابه بین دو گونهAe. truncialis و Ae. umbelulata و بین دو گونه Ae. neglecta و Ae. cylanrica مشاهده شد. تجزیه خوشه ای به‌طور مناسبی گونه ها را در گروه های مجزا تفکیک و تجزیه به مختصات اصلی نتایج تجزیه خوشه ای را تأیید کرد. با تجزیه ساختار جمعیت، نحوه جریان ژنی و اختلاط ژنتیکی بین گونه ها مشخص شد که در تطابق با نتایج تجزیه خوشه ای و نمودار دوبعدی PCoA بود. | ||
| کلیدواژه‌ها | ||
| گرامینه‌های مرتعی؛ تنوع ژنتیکی؛ گندم وحشی؛ نشانگر هدفمند؛ ژنهای مقاومت | ||
| عنوان مقاله [English] | ||
| Fingerprinting of Aegilops species using genes-targeted drived and conserved regiens CoRAP markers | ||
| نویسندگان [English] | ||
| S. Fabriki-Ourang1؛ H. Karimi2؛ J. Ahmadi3؛ A.A. Mehrabi4 | ||
| 1Assoc. Prof. Department of Genetics & Plant Breeding, Imam Khomeini International University, Qazvin, Iran. | ||
| 2M.Sc. of Genetics and Plant Breeding, Imam Khomeini International University, Qazvin, Iran. | ||
| 3Prof., Department of Genetics & Plant Breeding, Imam Khomeini International University, Qazvin, I.R. Iran. | ||
| 4Assoc. Prof. Dept. Biotechnology, Research Institute of Forest and rangelands, Agricultural Research, Education and Extention Organization (AREEO), Tehran, Iran. | ||
| چکیده [English] | ||
| Aegilops range grasses (Poaceae family) as one of the most important ancestors of wheat is a rich source of stresses tolerance genes. For this, the fingerprinting of genetic relationships in accessions belonging to eight species of Aegilops was studied using targeted genes-related CoRAP molecular markers. In designing the fixed primers, six genes CAT, MnSoD, SoS1, miR398, miR160b and miR169gR responsible for abiotic stresses tolerance were used. The lowest and highest polymorphic information contents were related to CoRAP2 and CoRAP10 primers with 0.92 and 0.96, respectively. The marker index varied from 6.89 in CoRAP7 primer to 13.49 in CoRAP9 primer. Low gene flow (Nm) and high differentiation (Gst) was observed between species. Also, the Fst index equal to 0.45 showed that the studied populations were completely separated. Due to the high values of the effective allele number and Nei genetic diversity, two species Ae. cylandrica and Ae. caudata showed high intra-specific diversity. The greatest similarity was observed between two species Ae. truncialis and Ae. umbelulata as well as Ae. neglecta and Ae. cylanrica. Cluster analysis appropriately divided species into distinct groups, and principal coordinate analysis confirmed the results of cluster analysis. Using structure analysis of the population, the mode of gene flow and genetic intermixture among species were in accordance with the grouping results of cluster analysis and PCoA biplot. | ||
| کلیدواژه‌ها [English] | ||
| Genetic diversity, Range grasses, Resistance genes, Targeted markers, Wild wheat | ||
| مراجع | ||
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