Comparison between Antimicrobial and Antibiofilm Activity of Exopolysaccharides (EPS) Extracted from Lactobacillus reuteri and Streptococcus Mitis against Oral Bacteria

M. Abed, Saif; Jabar Jasim, Hussein; Mijbas Mohammed Alomari, Mohammed; Hamoodi Hussein, Ghina

doi:10.22092/ari.2022.358341.2203

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	Comparison between Antimicrobial and Antibiofilm Activity of Exopolysaccharides (EPS) Extracted from Lactobacillus reuteri and Streptococcus Mitis against Oral Bacteria
Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 02 اردیبهشت 1401
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358341.2203
نویسندگان
Saif M. Abed ¹؛ Hussein Jabar Jasim²؛ Mohammed Mijbas Mohammed alomari²؛ Ghina Hamoodi Hussein³
¹Department of Medical laboratories, College of Medical and Health Techniques, Sawa University
²Iraq; 2College of Veterinary medicine/ Al-Muthanna University/ Iraq
³3Department of Medical laboratories, College of Medical and Health Techniques, Sawa University
چکیده
Due to the increased resistance to antibiotics and chemical biocides, the use of bacterial exopolysaccharides has been considered. The objective of the current study was to investigate strength of the antibacterial and antibiofilm activity of EPS extracted from Lactobacillus reuteri and Streptococcus miti because previous studies showed there was structural differences between EPS , during this study EPS extracted from Lactobacillus reuteri and Streptococcus Miti by ethanol precipitation method and estimated antibacterial and antibiofilm activity against several Oral Bacteria(Staphylococcus aureus, Staphylococcus hominis , Acinetobacter baumannii, Raoultella ornithinolytica , Streptococcus thoraltensis in different concentration as (100,150,200,250,300 mg/ml ),the results showed carbohydrates rate in extracted EPS from L.reuteri and S. mitis were recorded was 85 , 80 % respectively. The concentration 100 and 150 mg/ml for EPS from L.reuteri and S. mitis, there was no inhibitory effect, except in S.aureus(1.1±0.10) and S.hominis (1.0±0.10) at 100 mg/ml ,3.1±2.01, 2.1±0.54 mg/ml respectively at 150 mg/ml concentration but no significant differences (p≤ 0.05). However the anti-bacterial effect of those EPSs started at the concentration of 200 and upwards, where different results were recorded between the concentrations of both EPSs against all bacteria isolated (p≤ 0.05 ), On the other hand, the effect EPS from L.reuteri and S. mitis was clear against the formation of biofilm compared with the control , worth mentioning that EPS from L.reuteri was more effective compared with EPS from S. mitis in all isolates (p≤ 0.05 ) except for S. thoraltensis where it was noted that the EPS from S. mitis is more effective than EPS from L.reuteri . Through the results obtained in this study, it was noted that the difference in the structural nature of EPS has an important role in its effectiveness as an anti-bacterial and anti-biofilm formation and, as it was found that the EPS from L.reuteri showed more effective than EPS from S. mitis and thus the mechanism of preventing and inhibiting bacteria depending of the structural natural of EPS.
کلیدواژه‌ها
Keywords: EPS؛ Lactobacillus reuteri؛ Streptococcus Mitis؛ Antibacterial؛ Antibiofilm

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Comparison between Antimicrobial and Antibiofilm Activity of Exopolysaccharides (EPS) Extracted from Lactobacillus reuteri and Streptococcus Mitis against Oral Bacteria