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Mohammed, F, Baydaa Abed Hussein, A, Ahmed, T. (1401). Evaluation of Methylation Panel in the Promoter Region of p16INK4a, RASSF1A, and MGMT as a Biomarker in Sputum for Lung Cancer. سامانه مدیریت نشریات علمی, 77(3), 1075-1081. doi: 10.22092/ari.2022.357985.2131
F Mohammed; A Baydaa Abed Hussein; T Ahmed. "Evaluation of Methylation Panel in the Promoter Region of p16INK4a, RASSF1A, and MGMT as a Biomarker in Sputum for Lung Cancer". سامانه مدیریت نشریات علمی, 77, 3, 1401, 1075-1081. doi: 10.22092/ari.2022.357985.2131
Mohammed, F, Baydaa Abed Hussein, A, Ahmed, T. (1401). 'Evaluation of Methylation Panel in the Promoter Region of p16INK4a, RASSF1A, and MGMT as a Biomarker in Sputum for Lung Cancer', سامانه مدیریت نشریات علمی, 77(3), pp. 1075-1081. doi: 10.22092/ari.2022.357985.2131
Mohammed, F, Baydaa Abed Hussein, A, Ahmed, T. Evaluation of Methylation Panel in the Promoter Region of p16INK4a, RASSF1A, and MGMT as a Biomarker in Sputum for Lung Cancer. سامانه مدیریت نشریات علمی, 1401; 77(3): 1075-1081. doi: 10.22092/ari.2022.357985.2131

Evaluation of Methylation Panel in the Promoter Region of p16INK4a, RASSF1A, and MGMT as a Biomarker in Sputum for Lung Cancer

Archives of Razi Institute
مقاله 19، دوره 77، شماره 3، مرداد و شهریور 2022، صفحه 1075-1081    اصل مقاله (342.72 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357985.2131
نویسندگان
F Mohammed* 1؛ A Baydaa Abed Hussein2؛ T Ahmed1
1AL-Manara College for Medical Sciences, Department of Pharmacy, Maysan, Iraq
2Department of Sciences, College of Basic Education, University of Misan, Maysan, Iraq
چکیده
Lung cancer is the most common cause of cancer death in the world. Effective early detection and appropriate medications can help treat this deadly cancer. Therefore, early detection of lung cancer is of utmost importance, especially in screening high-risk populations (such as smokers) with an urgent need to identify new biomarkers. The present study aimed to demonstrate the potential of using the panel of DNA methylation as a biomarker for the early diagnosis of lung cancer from sputum samples. The methylated promoter of p16INK4a, RASSF1A, and MGMT genes was estimated by the methylation-specific polymerase chain reaction in a sample of 84 lung cancer patients (65 smokers and 19 non-smokers). Based on the results, p16INK4a promoter methylation was significantly associated with smoking habit and lung cancer progression in terms of histological grading and patient staging. The sensitivity and specificity of the p16INK4a gene as a biomarker for lung cancer were 71% and 90%, respectively. The methylated promoter of RASSF1A was less sensitive (48%) as a biomarker for lung cancer with 83%. The results demonstrated a strong association between promoter methylation of RASSF1A and late stages of lung cancer (P=0.0007). The sensitivity of the MGMT gene as a biomarker for lung cancer was 61% with high specificity (92%), compared to other candidate genes in this study. The epigenetic alteration in the promoter region of p16INK4a, RASSF1A, and MGMT genes is highly associated with cancer cell development. It is suggested that the use of these candidate biomarkers can be used as an adjunct to computed tomography screening to diagnose patients at high risk for lung cancer after validation.
کلیدواژه‌ها
Biomarker؛ DNA methylation؛ Lung cancer؛ Tumor suppressor gene
مراجع
  1. Sanders HR, Albitar M. Somatic mutations of signaling genes in non-small-cell lung cancer. Cancer Genet Cytogenet. 2010;203(1):7-15.
  2. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394-424.
  3. Team NLSTR. Reduced lung-cancer mortality with low-dose computed tomographic screening. N Engl J Med. 2011;365(5):395-409.
  4. Silvestri GA, Vachani A, Whitney D, Elashoff M, Porta Smith K, Ferguson JS, et al. A bronchial genomic classifier for the diagnostic evaluation of lung cancer. N Engl J Med. 2015;373:243-51.
  5. Rivera MP, Mehta AC, Wahidi MM. Establishing the diagnosis of lung cancer: Diagnosis and management of lung cancer: American College of Chest Physicians evidence-based clinical practice guidelines. Chest. 2013;143(5): 142S-65S.
  6. Su Y, Fang H, Jiang F. Integrating DNA methylation and microRNA biomarkers in sputum for lung cancer detection. Clinical epigenetics. 2016;8(1):1-9.
  7. Su Y, Fang HB, Jiang F. An epigenetic classifier for early stage lung cancer. Clin Epigenetics. 2018;10(1):1-9.
  8. Llinas-Arias P, Esteller M. Epigenetic inactivation of tumour suppressor coding and non-coding genes in human cancer: an update. Open Biol. 2017;7(9):170152.
  9. Liyanage C, Wathupola A, Muraleetharan S, Perera K, Punyadeera C, Udagama P. Promoter hypermethylation of tumor-suppressor genes p16INK4a, RASSF1A, TIMP3, and PCQAP/MED15 in salivary DNA as a quadruple biomarker panel for early detection of oral and oropharyngeal cancers. Biomolecules. 2019;9(4):148.
  10. Powrozek T, Malecka-Massalska T. DNA hypermethylation of tumor suppressor genes as an early lung cancer biomarker. Transl Cancer Res. 2016;5:S1531-S8.
  11. Palmisano WA, Divine KK, Saccomanno G, Gilliland FD, Baylin SB, Herman JG, et al. Predicting lung cancer by detecting aberrant promoter methylation in sputum. Cancer Res. 2000;60(21):5954-8.
  12. Liu Y, Lan Q, Siegfried JM, Luketich JD, Keohavong P. Aberrant promoter methylation of p16 and MGMT genes in lung tumors from smoking and never-smoking lung cancer patients. Neoplasia. 2006;8(1):46-51.
  13. Keller M, Dubois F, Teulier S, Martin AP, Levallet J, Maille E, et al. NDR2 kinase contributes to cell invasion and cytokinesis defects induced by the inactivation of RASSF1A tumor-suppressor gene in lung cancer cells. J Exp Clin Cancer Res. 2019;38(1):1-16.
  14. Cabrini G, Fabbri E, Lo Nigro C, Dechecchi MC, Gambari R. Regulation of expression of O6-methylguanine-DNA methyltransferase and the treatment of glioblastoma. Int J Oncol. 2015;47(2):417-28.
  15. Jabbara N, Asaadi Tehrani G, Lalooha F, Farzam SA, Elmizadeh K. Promoter hypermethylation analysis of the tumor suppressor genes RASSF1A and RASSF2A in Iranian endometrial carcinoma patients. Int J Cancer Manag. 2017;10(4).
  16. Ettinger DS, Wood DE, Akerley W, Bazhenova LA, Borghaei H, Camidge DR, et al. Non–small cell lung cancer, version 1.2015. J Natl Compr Cancer Netw. 2014;12(12):1738-61.
  17. Hulbert A, Jusue-Torres I, Stark A, Chen C, Rodgers K, Lee B, et al. Early detection of lung cancer using DNA promoter hypermethylation in plasma and sputum. Clin Cancer Res. 2017;23(8):1998-2005.
  18. Leng S, Do K, Yingling CM, Picchi MA, Wolf HJ, Kennedy TC, et al. Defining a gene promoter methylation signature in sputum for lung cancer risk assessment. Clin Cancer Res. 2012;18(12):3387-95.
  19. Kennedy TC, Proudfoot SP, Piantadosi S, Wu L, Saccomanno G, Petty TL, et al. Efficacy of two sputum collection techniques in patients with air flow obstruction. Acta cytologica. 1999;43(4):630-6.
  20. Belinsky SA, Palmisano WA, Gilliland FD, Crooks LA, Divine KK, Winters SA, et al. Aberrant promoter methylation in bronchial epithelium and sputum from current and former smokers. Cancer Res. 2002;62(8):2370-7.
  21. Esteller M, Hamilton SR, Burger PC, Baylin SB, Herman JG. Inactivation of the DNA repair gene O6-methylguanine-DNA methyltransferase by promoter hypermethylation is a common event in primary human neoplasia. Cancer Res. 1999;59(4):793-7.
  22. Byrnes SA, Weigl BH. Selecting analytical biomarkers for diagnostic applications: a first principles approach. Expert Review of Molecular Diagnostics. 2018;18(1):19-26.
  23. Belinsky SA, Liechty KC, Gentry FD, Wolf HJ, Rogers J, Vu K, et al. Promoter hypermethylation of multiple genes in sputum precedes lung cancer incidence in a high-risk cohort. Cancer Res. 2006;66(6):3338-44.
  24. Lissa D, Robles AI. Sputum-based DNA methylation biomarkers to guide lung cancer screening decisions. J Thorac Dis. 2017;9(11):4308.
  25. Roessler J, Lehmann U. Quantitative DNA methylation analysis by pyrosequencing®. Pyrosequencing: Springer; 2015. p. 175-88.
  26. Banoon SR, Ghasemian A. The characters of graphene oxide nanoparticles and doxorubicin against HCT-116 colorectal cancer cells in vitro. J Gastrointest Cancer. 2021:1-5.
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