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Qaysar Musa, S, Mohammed AliJassim, M, Mohammed Mahmood, M. (1401). Tracing Some Salivary Immune Elements in Iraqi SARS-2 Patients. سامانه مدیریت نشریات علمی, 77(5), 1587-1591. doi: 10.22092/ari.2022.358330.2195
S Qaysar Musa; M Mohammed AliJassim; M Mohammed Mahmood. "Tracing Some Salivary Immune Elements in Iraqi SARS-2 Patients". سامانه مدیریت نشریات علمی, 77, 5, 1401, 1587-1591. doi: 10.22092/ari.2022.358330.2195
Qaysar Musa, S, Mohammed AliJassim, M, Mohammed Mahmood, M. (1401). 'Tracing Some Salivary Immune Elements in Iraqi SARS-2 Patients', سامانه مدیریت نشریات علمی, 77(5), pp. 1587-1591. doi: 10.22092/ari.2022.358330.2195
Qaysar Musa, S, Mohammed AliJassim, M, Mohammed Mahmood, M. Tracing Some Salivary Immune Elements in Iraqi SARS-2 Patients. سامانه مدیریت نشریات علمی, 1401; 77(5): 1587-1591. doi: 10.22092/ari.2022.358330.2195

Tracing Some Salivary Immune Elements in Iraqi SARS-2 Patients

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مقاله 10، دوره 77، شماره 5، آذر و دی 2022، صفحه 1587-1591    اصل مقاله (304.04 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358330.2195
نویسندگان
S Qaysar Musa1؛ M Mohammed AliJassim1؛ M Mohammed Mahmood* 2
1College of Dentistry, Al-Muthanna University, Al-Muthanna, Iraq
2Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq
چکیده
Saliva is one of the most significant components in maintaining oral homeostasis and symbiosis. It contains antimicrobial proteins and peptides, such as mucins, lactoferrin, lysozyme, lactoperoxidase, Catherine, statins, and antibodies (secretory immunoglobin A [sIgA]). Early defenses against respiratory infections rely heavily on mucosal immunity, especially secretory sIgA, which has several features and functions that make it suitable for mucosal defense. Salivary testing has been utilized to define mucosal immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Lysozyme has muramidase, with antimicrobial activity, and high concentrations in body fluids, such as saliva and tear. This research aimed to offer an update on how saliva components suppress viral infection and sustain health. A total of 50 individuals, including 30 SARS-2 patients and 20 non-infected subjects, in the age range of 32-54 years were enrolled in this study. Saliva specimens were obtained from polymerase chain reaction (PCR)-confirmed coronavirus disease 2019 (COVID-19) patients and non-infected participants. To collect saliva, the subjects were advised to swirl water over their lips three times, and 5.0 ml of saliva was collected. Samples were centrifuged at 800 x g for 10 min. Saliva was diluted at 1:2,000 with 1 × Diluent N. The immunoglobulin A (IgA) titer in saliva was detected. A spectrophotometer was used to measure the solution's change in absorbance at 550 nm. Measurements (salivary IgA and lysozyme) were made after 7, 30, and 60 days of confirmatory PCR COVID-19 test. The mean scores of salivary IgA levels were obtained at 17.85, 15.26, and 10.73 mg/dl in patients and 9.53, 10.33, and 9.21 mg/dl in healthy individuals after 7, 30, and 60 days, respectively. The salivary lysozyme activity levels in SARS-2 patients compared to controls were 9.7, 7.3, and 4.2 mg/dl versus 2.9, 3.4, and 3.77 mg/dl, respectively. The salivary IgA level was significantly higher in patients of a confirmatory test for COVID-19 compared to healthy individuals.
کلیدواژه‌ها
COVID-19؛ IgA؛ Lysozyme؛ SARS-2
مراجع
  1. Galeas-Pena M, McLaughlin N, Pociask D. The role of the innate immune system on pulmonary infections. Biol Chem. 2019;400(4):443-56.
  2. Marshall JS, Portales-Cervantes L, Leong E. Mast Cell Responses to Viruses and Pathogen Products. Int J Mol Sci. 2019;20(17).
  3. Papageorgiou AC, Mohsin I. The SARS-CoV-2 Spike Glycoprotein as a Drug and Vaccine Target: Structural Insights into Its Complexes with ACE2 and Antibodies. Cells. 2020;9(11).
  4. Varadhachary A, Chatterjee D, Garza J, Garr RP, Foley C, Letkeman AF, et al. Salivary anti-SARS-CoV-2 IgA as an accessible biomarker of mucosal immunity against COVID-19. medRxiv. 2020.
  5. Ceron JJ, Lamy E, Martinez-Subiela S, Lopez-Jornet P, Capela ESF, Eckersall PD, et al. Use of Saliva for Diagnosis and Monitoring the SARS-CoV-2: A General Perspective. J Clin Med. 2020;9(5).
  6. Sterlin D, Mathian A, Miyara M, Mohr A, Anna F, Claer L, et al. IgA dominates the early neutralizing antibody response to SARS-CoV-2. Sci Transl Med. 2021;13(577).
  7. Wang T, Riegger A, Lamla M, Wiese S, Oeckl P, Otto M, et al. Water-soluble allyl sulfones for dual site-specific labelling of proteins and cyclic peptides. Chem Sci. 2016;7(5):3234-9.
  8. Chao YX, Rotzschke O, Tan EK. The role of IgA in COVID-19. Brain Behav Immun. 2020;87:182-3.
  9. Shah VK, Firmal P, Alam A, Ganguly D, Chattopadhyay S. Overview of Immune Response During SARS-CoV-2 Infection: Lessons From the Past. Front Immunol. 2020;11:1949.
  10. Pietrzak B, Tomela K, Olejnik-Schmidt A, Mackiewicz A, Schmidt M. Secretory IgA in Intestinal Mucosal Secretions as an Adaptive Barrier against Microbial Cells. Int J Mol Sci. 2020;21(23).
  11. Sanjabi S, Oh SA, Li MO. Regulation of the Immune Response by TGF-beta: From Conception to Autoimmunity and Infection. Cold Spring Harb Perspect Biol. 2017;9(6).
  12. Alroy J, Lyons JA. Lysosomal Storage Diseases. J Inborn Errors Metab Screen. 2014;2.
  13. Gordon LI, Douglas SD, Kay NE, Yamada O, Osserman EF, Jacob HS. Modulation of neutrophil function by lysozyme. Potential negative feedback system of inflammation. J Clin Invest. 1979;64(1):226-32. 
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