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Waheed Khudiar khareeb, Zainab, Ismail Ibrahim, Zainab, Ali Abdullah, Fawziah. (1401). Pathological and Molecular detection of Mycoplasma ovipneumoneae in Sheep of Basrah Province. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2022.357996.2134
Zainab Waheed Khudiar khareeb; Zainab Ismail Ibrahim; Fawziah Ali Abdullah. "Pathological and Molecular detection of Mycoplasma ovipneumoneae in Sheep of Basrah Province". سامانه مدیریت نشریات علمی, , , 1401, -. doi: 10.22092/ari.2022.357996.2134
Waheed Khudiar khareeb, Zainab, Ismail Ibrahim, Zainab, Ali Abdullah, Fawziah. (1401). 'Pathological and Molecular detection of Mycoplasma ovipneumoneae in Sheep of Basrah Province', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2022.357996.2134
Waheed Khudiar khareeb, Zainab, Ismail Ibrahim, Zainab, Ali Abdullah, Fawziah. Pathological and Molecular detection of Mycoplasma ovipneumoneae in Sheep of Basrah Province. سامانه مدیریت نشریات علمی, 1401; (): -. doi: 10.22092/ari.2022.357996.2134

Pathological and Molecular detection of Mycoplasma ovipneumoneae in Sheep of Basrah Province

Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 23 فروردین 1401  XML
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357996.2134
نویسندگان
Zainab Waheed Khudiar khareeb email 1؛ Zainab Ismail Ibrahim2؛ Fawziah Ali Abdullah3
11Department of Pathology and Poultry diseases/University of Basrah-College of Veterinary Medicine
22Department of Pathology and Poultry diseases/University of Baghdad-College of Veterinary Medicine
33Department of Microbiology /University of Basrah-College of Veterinary Medicine
چکیده
In vitro fertilization (IVF) is regarded as the most important reproductive biotechnological approach having great potential to accelerate genetic improvement in ruminant animals and also for the research of embryonic development. The study aimed to show the effect of culture media and the addition of natural and synthetic oxidants on in vitro maturation (IVM), fertilization (IVF), and in vitro embryo culture (IVC) in of Local ewes (Iraq). 304 genital systems of locals ewes were collected from Fallujah slaughterhouse/Fallujah/AL-Anbar province during the period from 3, January to 1, July 2021. The study was conducted on Reproductive Biotechnology Lab. Department of Surgery and Theriogenology, College of Veterinary Medicine/ University of Fallujah. From 608 ovaries, 1368 Oocytes were recovered which is surrounded with cumulus Cells. Aspiration was the method of collection Oocytes were divided into eight treatments, The first treatment (T1) was MEM + Capparis Spinosa extract 50µmol. The 2nd treatment (T2) was MEM + Silymarin extract 100µmol. The 3rd treatment (T3) was MEM + Coenzyme Q10 5 µmol. The fourth treatment (T4) was MEM only serve as a control. The 5th treatment (T5) Was DMEM + Capparis Spinosa extract 50 µmol. The 6th treatment (T6) was DMEM + silymarin extract 100µmol.The seventh treatment (T7) was DMEM + Coenzyme Q10 5µmol. The 8eth treatment (T8) was DMEM Only and Serve as a control. The results showed that there was a significant difference (P≤0.05) between T5 (DMEM + Capparis Spinosa extract) and others Control or treated treatments. in the Morula stage and blastocyst stage. It was concluded from this study that cultural media DMEM with Capparis spinosa (as antioxidant) give the best results.
کلیدواژه‌ها
immunity؛ Mycoplasma ovipneumonia؛ pathology؛ PCR؛ sheep
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تعداد مشاهده مقاله: 13


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