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Maikan, H. K, Jabbar, S, Al-Haishawi, H. (1401). Isolation and Identification of Candida tropicalis as a Cause of Cutaneous Candidiasis in Kalar District, Iraq. سامانه مدیریت نشریات علمی, 77(4), 1377-1382. doi: 10.22092/ari.2022.357613.2066
H. K Maikan; S Jabbar; H Al-Haishawi. "Isolation and Identification of Candida tropicalis as a Cause of Cutaneous Candidiasis in Kalar District, Iraq". سامانه مدیریت نشریات علمی, 77, 4, 1401, 1377-1382. doi: 10.22092/ari.2022.357613.2066
Maikan, H. K, Jabbar, S, Al-Haishawi, H. (1401). 'Isolation and Identification of Candida tropicalis as a Cause of Cutaneous Candidiasis in Kalar District, Iraq', سامانه مدیریت نشریات علمی, 77(4), pp. 1377-1382. doi: 10.22092/ari.2022.357613.2066
Maikan, H. K, Jabbar, S, Al-Haishawi, H. Isolation and Identification of Candida tropicalis as a Cause of Cutaneous Candidiasis in Kalar District, Iraq. سامانه مدیریت نشریات علمی, 1401; 77(4): 1377-1382. doi: 10.22092/ari.2022.357613.2066

Isolation and Identification of Candida tropicalis as a Cause of Cutaneous Candidiasis in Kalar District, Iraq

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مقاله 9، دوره 77، شماره 4، مهر و آبان 2022، صفحه 1377-1382    اصل مقاله (640.39 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357613.2066
نویسندگان
H. K Maikan* 1؛ S Jabbar2؛ H Al-Haishawi3
1Department of Biology, College of Education, University of Garmian, Sulaymaniyah, Iraq
2Department of Biology, College of Science, University of Kirkuk, Kirkuk, Iraq
3Department of Physiology, College of Medicine, University of Misan, Misan, Iraq
چکیده
Fungal infections are currently causing health issues all over the world, among which are Candida species that cause cutaneous infection. Numerous dermatological studies concentrated on a single species. However, the virulence factors and the spread of specific candidiasis in specific areas have remained poorly understood. Therefore, the current study was designed to shed light on Candida tropicalis, which has been identified as the most prevalent yeast among Candida non-albicans species. Forty specimens were collected from patients with cutaneous fungal infection (25 females and 15 males) and underwent examination. According to conventional identification based on macroscopic and microscopic examinations, eight isolates were identified as C. tropicalis from Candida non-albicans. Molecular diagnosis for internal transcribed spacers (ITS1 and ITS4) using conventional polymerase chain reaction (PCR) yielded an amplicon of 520 bp for all isolates. Further investigation of PCR-restriction fragment length using Mitochondrial sorting protein; Msp1 enzyme revealed two bands of 340 and 180 bp. The ITS gene sequence in one isolated species was found to be 98% identical to C. tropicalis strain MYA-3404 chromosome R ATCC CP047875.1. Another isolate shared 98.02% identity with C. tropicalis strain MA6 18S ribosomal RNA gene DQ666188.1, indicating C. tropical species identity, implying that non-Candida species should be considered when diagnosing candidiasis. This study demonstrated the significance of Candida non-albicans, particularly C. tropicalis, in terms of pathogenic potential, the ability to cause potentially fatal systemic infections and candidiasis, and acquired flucozonal resistance with a high mortality rate.
کلیدواژه‌ها
Candidiasis؛ Candida tropicalis؛ ITS؛ PCR-RFLP
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مراجع
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