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محتوای ترکیب‌های فنلی و فعالیت آنزیم‌های PAL و PPO گیاه ریحان سبز (Ocimum basilicum L.) با پیش‌تیمار کروناتین تحت سمّیت آرسنیک (As) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 6، دوره 31، شماره 1 - شماره پیاپی 69، فروردین 1394، صفحه 52-62 اصل مقاله (414.35 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2015.12610 | ||
| نویسندگان | ||
| سعید زارع ده‌آبادی* 1؛ زهرا اسرار2؛ عبدالحمید نمکی شوشتری3؛ میترا مهربانی4 | ||
| 1دانشجوی دکتری، گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهید باهنر کرمان | ||
| 2دانشیار، گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهید باهنر کرمان | ||
| 3استادیار، گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهید باهنر کرمان | ||
| 4دانشیار، گروه فارماکوگنوزی، دانشکده داروسازی، دانشگاه علوم پزشکی کرمان | ||
| چکیده | ||
| افزایش ذخیره آنتی‌اکسیدانی به‌خصوص تجمع محتوای ترکیب‌های فنلی یکی از مهمترین راهکارهای غیرآنزیمی گیاهان برای مقابله در برابر تنش‌های محیطی می‌باشد. در این پژوهش نمونه‌های ریحان سبز (Ocimum basilicum L.) در شرایط گلخانه‌ای تحت تیمار غلظت‌های مختلف کروناتین (0، 50 و 100 نانومولار) به‌عنوان پیش‌تیمار و فلز سنگین آرسنیک (0، 150 و 300 میکرومولار) رشد داده شدند. سپس شاخص‌های مورد نظر در نمونه‌های تیمار شده اندازه‌گیری شد. افزایش میزان پراکسیداسیون لیپیدها و نشت یونی غشاء و تجمع بالای پراکسید هیدروژن در برگ‌های ریحان از علائم سمّیت آرسنات بود که در اثر استفاده از پیش‌تیمار کروناتین به‌طور معنی‌داری از مقدار آنها کاسته شد. تجمع ترکیب‌های فنلی، آنتوسیانین‌ها، فلاونوییدها و همچنین افزایش فعالیت آنزیم‌های فنیل‌آلانین آمونیالیاز (PAL) و پلی‌فنل اکسیداز (PPO) توسط کروناتین در نمونه‌های تحت سمّیت آرسنیک نشان می‌دهد که شاید کروناتین به‌عنوان یک ترکیب مشابه با جاسمونات‌ها از طریق افزایش ذخیره آنتی‌اکسیدانی گیاه ریحان مقاومت آن را در برابر سمّیت فلز آرسنیک بهبود بخشیده است. | ||
| کلیدواژه‌ها | ||
| آرسنیک؛ کروناتین؛ ترکیب‌های فنلی؛ PAL؛ PPO | ||
| عنوان مقاله [English] | ||
| Phenolics content and PAL and PPO activity of Ocimum basilicum L. with coronatine pretreatment under arsenic toxicity | ||
| نویسندگان [English] | ||
| S. Zare Dehabadi1؛ Z. Asrar2؛ A. Shoushtari3؛ M. Mehrabani4 | ||
| 1Ph.D Student, Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman | ||
| 2Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| 3Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| 4Department of Pharmacognozy, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran | ||
| چکیده [English] | ||
| Enhancing antioxidant pool especially phenolic compounds accumulation is one of the most important non enzymatic tools of plants to resist environmental stresses. In this investigation, basil (Ocimum basilicum L.) seedlings, grown in a control condition and irrigated with Hoagland nutrient solution, were treated withcoronatine (COR) at 0 (Control), 50 and 100 nM and different concentrations of As (V) at 0 (Control), 150 and 300 µM. Then some parameters in treated plants were measured. Increasing of lipid peroxidation and electrolyte leakage and H2O2 accumulation in basil leaves were the symptoms of arsenate toxicity that were ameliorated by COR pretreatment. Increasing phenolic compound contents, flavonoid and anthocyanin content and enhancing PAL and PPO activity indicate that COR as a jasmonates analogue may ameliorate arsenic toxicity in O. basilicum seedlings by improving antioxidative defense system. | ||
| کلیدواژه‌ها [English] | ||
| Arsenic, coronatine, Phenolic compounds, PAL, PPO | ||
| مراجع | ||
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