Evaluation of killed vaccine efficacy on hematology parameters and IgM assay of Acipenser stellatus (Pallas, 1771) challenged with Nervous Necrosis Virus (NNV)

Afsharipour, E; Azari Takami, E; Zorriehzahra, M.J; Motallebi, A.A; Kakoolaki, Sh

doi:10.22092/ijfs.2022.125963

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	Evaluation of killed vaccine efficacy on hematology parameters and IgM assay of Acipenser stellatus (Pallas, 1771) challenged with Nervous Necrosis Virus (NNV)
Iranian Journal of Fisheries Sciences
دوره 21، شماره 1، فروردین 2022، صفحه 301-315 اصل مقاله (844.62 K)
شناسه دیجیتال (DOI): 10.22092/ijfs.2022.125963
نویسندگان
E Afsharipour¹؛ E Azari Takami¹؛ M.J Zorriehzahra^* ²؛ A.A Motallebi³؛ Sh Kakoolaki²
¹Department of Basic Science and Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran
²Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran.
³Department of Basic Science and Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran.
چکیده
Vaccination is considered the most effective, environmentally friendly and cost-effective way of protecting commercial finfish species against infectious diseases with high economic impacts. Efficacy of a new inactivated Viral Nervous Necrosis (VNN) vaccine through heat-inactivated nervous necrosis virus (NNV) combined with three types of adjuvants was assessed in 7 g juvenile of Acipenser stellatus (Pallas, 1771) as a laboratory model. Ten groups of 30 juveniles were divided in 18 aquariums to vaccinate via immersion and injection routes using 4 types of vaccines. Vaccination was performed in two stages one month apart and one month after the second replication, exposure to the live virus was performed. During this period, the mortality rate of immersion and injection groups was 12.9% and 19.8%, respectively, compared to 100% mortality in the control group. Sampling was performed in four stages before the first vaccination in the adaptation period, after the first vaccination, after the second replication, and after exposure to live virus. Blood samples were measured to evaluate changes in blood factors (red blood cell count, white blood cell count, hematocrit and hemoglobin) and immunity factors (immunoglobulin M (IgM). In order to prove the virulence of the viral sample (supernatant) used in exposure, the pathogenicity of VNN virus isolated from infected Golden grey mullet (Chelon auratus) with (TCID₅₀ ) was examined in several guppies. One month after the second repetition of fish, all treatments were exposed to the acute virus sample (supernatant), and after one month, their survival percentage, blood factors and safety were measured.
کلیدواژه‌ها
Acipenser stellatus؛ ELISA؛ Immunization؛ Nervous Necrosis Virus؛ Vaccination

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آمار

Evaluation of killed vaccine efficacy on hematology parameters and IgM assay of Acipenser stellatus (Pallas, 1771) challenged with Nervous Necrosis Virus (NNV)