Jolodar, A, Farzi, M, Ezzati Givi, M, Jafari, H. (1400). Phylogenetic Relationships of Scorpion Compsobuthus matthiesseni Based on Sequences of Internal Transcribed Spacer 2 Gene from Khuzestan Province, Iran. سامانه مدیریت نشریات علمی, 77(1), 65-72. doi: 10.22092/ari.2021.355059.1662
A Jolodar; M Farzi; M Ezzati Givi; H Jafari. "Phylogenetic Relationships of Scorpion Compsobuthus matthiesseni Based on Sequences of Internal Transcribed Spacer 2 Gene from Khuzestan Province, Iran". سامانه مدیریت نشریات علمی, 77, 1, 1400, 65-72. doi: 10.22092/ari.2021.355059.1662
Jolodar, A, Farzi, M, Ezzati Givi, M, Jafari, H. (1400). 'Phylogenetic Relationships of Scorpion Compsobuthus matthiesseni Based on Sequences of Internal Transcribed Spacer 2 Gene from Khuzestan Province, Iran', سامانه مدیریت نشریات علمی, 77(1), pp. 65-72. doi: 10.22092/ari.2021.355059.1662
Jolodar, A, Farzi, M, Ezzati Givi, M, Jafari, H. Phylogenetic Relationships of Scorpion Compsobuthus matthiesseni Based on Sequences of Internal Transcribed Spacer 2 Gene from Khuzestan Province, Iran. سامانه مدیریت نشریات علمی, 1400; 77(1): 65-72. doi: 10.22092/ari.2021.355059.1662
Phylogenetic Relationships of Scorpion Compsobuthus matthiesseni Based on Sequences of Internal Transcribed Spacer 2 Gene from Khuzestan Province, Iran
1Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran
چکیده
Buthidae family includes scorpions with highly potent venom such as Compsobuthus matthiesseni is important due to the prevalence of scorpion stings in Khuzestan Province, Iran. Morphometric comparison of males and females (n=5 each) showed that the body and carapas of the females were longer and wider (32.57±0.23 mm and 3.75±0.22 mm, respectively) than those of males (28.89±0.25 mm and 3.55±0.12 mm, respectively). From the seven specimens of C. matthiesseni scorpion, 410-bp gene fragments of ribosomal internal transcribed spacer 2 were amplified by polymerase chain reaction. The specimens of CM1 and CM2 (isolated from Baghmalek, Khuzestan) were in the same group with bootstrap values of 87%. Nevertheless, CM4 and CM3 (isolated from Shushtar and Bidroobe, Khuzestan) with bootstrap values of 73% and 62% were separated from the two specimens of Baghmalek, respectively. The two specimens CF3 and CM5 (isolated from Masjed Soleiman, Khuzestan) with bootstrap values of 88% were placed next to each other in a separate group. CF2 was separated from the rest of the specimens with a bootstrap value of 54%. Out of the seven scorpions that were examined, six specimens (CM1, CM2, CM3, CM4, CM5, and CF3) showed the greatest similarity between 1.1% and 4%. However, the genetic distance between CF2 and the rest of the specimens was at the range of 10.8-14.2%. It can be concluded that all C.matthiesseni scorpions from Khuzestan Province belonged to one species; nonetheless, differences were observed within the species, especially in the case of CF2, which might be intraspecies.
فیلوژنی عقرب کمپسوبوتوس ماتینسنی بر اساس توالی ژن فاصله انداز رونویسی شونده داخلی 2 (ITS2) از خوزستان
چکیده [English]
عقربه های خانواده بوتیده، نظیر کمپسوبوتوس ماتیسنی که بدلیل داشتن سم بسیار قوی و شیوع گزش در استان خوزستان حائز اهمیت اند. مقایسه ریخت سنجی عقرب نر (n = 5) و ماده (n = 2) نشان داد که اندازه بدن ماده بلندتر (32.57±0.23mm) و کاراپاس آن پهن تر (3.75±0.22) نسبت به عقرب نر (بترتیب 28.89±0.25mm ,و (3.55±0.12mm می باشد. به کمک PCR ، از هفت نمونه عقرب کمپسوبوتوس ماتیسنی، قطعات ژنی به میزان 410 جفت باز از ژن فاصله انداز رونویسی شونده داخلی 2 (ITS2) افزوده سازی گردید. نمونه های CM1 و CM2 (جدا شده از باغملک) با بوت استرپ 87 درصد در یک گروه قرار گرفتند. در حالیکه CM4 (جدا شده از شوشتر) و CM3 (جدا شده از بیدروبه) با مقادیر بوت استرپ بترتیب 73 و 62 درصد، از دو نمونه باغملک جدا شدند. دو نمونه CF3 و CM5 (جدا شده از مسجدسلیمان) با بوت استرپ 88 در کنار یکدیگر در یک گروه جداگانه قرار گرفتند. نمونه CF2 از بقیه نمونه ها با بوت استرپ 54 بطور جداگانه در یک گروه قرار گرفت. از بین هفت نمونه عقرب مورد بررسی، 6 نمونه (CM1 ، CM2 ، CM3 ، CM4 ، CM5 و CF3) بیشترین شباهت را بین 1.1 - 4 درصد نشان دادند. هرچند که فاصله ژنتیکی بین CF2 و بقیه نمونه ها بین 10.8-14.2 بود. می توان چنین نتیجه گرفت که همه عقرب های کمپسوبوتوس ماتیسنی مورد مطالعه در این پژوهش متعلق به یک گونه اند، گرچه ممکن است تفاوت نمونه CF2 با بقیه نمونه ها، یک تفاوت درون گونه ای باشد.
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