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Salehi-Najafabadi, Z, Goudarzi, H. R, Sajadi, M. (1400). Evaluation of in vivo Lethality and in vitro Cytotoxic Effect of Odontobuthus bidentatus Scorpion Venom. سامانه مدیریت نشریات علمی, 77(1), 29-36. doi: 10.22092/ari.2021.353302.1595
Z Salehi-Najafabadi; H. R Goudarzi; M Sajadi. "Evaluation of in vivo Lethality and in vitro Cytotoxic Effect of Odontobuthus bidentatus Scorpion Venom". سامانه مدیریت نشریات علمی, 77, 1, 1400, 29-36. doi: 10.22092/ari.2021.353302.1595
Salehi-Najafabadi, Z, Goudarzi, H. R, Sajadi, M. (1400). 'Evaluation of in vivo Lethality and in vitro Cytotoxic Effect of Odontobuthus bidentatus Scorpion Venom', سامانه مدیریت نشریات علمی, 77(1), pp. 29-36. doi: 10.22092/ari.2021.353302.1595
Salehi-Najafabadi, Z, Goudarzi, H. R, Sajadi, M. Evaluation of in vivo Lethality and in vitro Cytotoxic Effect of Odontobuthus bidentatus Scorpion Venom. سامانه مدیریت نشریات علمی, 1400; 77(1): 29-36. doi: 10.22092/ari.2021.353302.1595

Evaluation of in vivo Lethality and in vitro Cytotoxic Effect of Odontobuthus bidentatus Scorpion Venom

Archives of Razi Institute
مقاله 6، دوره 77، شماره 1، فروردین و اردیبهشت 2022، صفحه 29-36    اصل مقاله (500.81 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.353302.1595
نویسندگان
Z Salehi-Najafabadi* 1؛ H. R Goudarzi1؛ M Sajadi2
1Department of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2Department of Biology, Tofigh Daru Research and Engineering Company, Tehran, Iran
چکیده
The results of numerous studies have revealed that some deadly scorpion venoms are composed of various bioactive molecules that have significant cytotoxic effects on cancer cells. In this study, the in vivo lethality and cytotoxic effect of Odontobuthus bidentatus venom were evaluated in different cancer cell lines. Through MTT assay, the cytotoxic effects of O. bidentatus scorpion venom were analyzed on the MCF-7, A549, AGS, HepG2, and Ht-29 cancer cell lines and Hu02 normal cells. To this end, six venom fractions were obtained through a Sephadex G-50 column, and the cytotoxic effects of isolated fractions were evaluated on A549 lung cancer cells.  The median lethal dose of O. bidentatus scorpion venom was determined at 0.73 mg/kg by intravenous administration of different venom doses in male BALB/c mice according to the Spearman-Karber method. The O. bidentatus scorpion whole venom had a significant cytotoxic effect on MCF-7, A549, and AGS cells. The treatment of A549 cells with various concentrations of fraction F1 showed that this fraction significantly induced growth inhibitory effect on the cells in a dose-dependent manner, compared to untreated cells.
کلیدواژه‌ها
Odontobuthus bidentatus venom؛ Cytotoxic effects؛ LD50؛ Cancer cells
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