اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,044
تعداد مقالات34,047
تعداد مشاهده مقاله46,439,514
تعداد دریافت فایل اصل مقاله77,507,276
Alborzi, Alireza, Bahrami, S, Khajeh, F. (1400). A Preliminary Study on the Antigenic Proteins of Linguatula serrata Nymphal Stage in Sheep with Visceral Linguatulosis. سامانه مدیریت نشریات علمی, 77(1), 23-28. doi: 10.22092/ari.2021.352744.1578
Alireza Alborzi; S Bahrami; F Khajeh. "A Preliminary Study on the Antigenic Proteins of Linguatula serrata Nymphal Stage in Sheep with Visceral Linguatulosis". سامانه مدیریت نشریات علمی, 77, 1, 1400, 23-28. doi: 10.22092/ari.2021.352744.1578
Alborzi, Alireza, Bahrami, S, Khajeh, F. (1400). 'A Preliminary Study on the Antigenic Proteins of Linguatula serrata Nymphal Stage in Sheep with Visceral Linguatulosis', سامانه مدیریت نشریات علمی, 77(1), pp. 23-28. doi: 10.22092/ari.2021.352744.1578
Alborzi, Alireza, Bahrami, S, Khajeh, F. A Preliminary Study on the Antigenic Proteins of Linguatula serrata Nymphal Stage in Sheep with Visceral Linguatulosis. سامانه مدیریت نشریات علمی, 1400; 77(1): 23-28. doi: 10.22092/ari.2021.352744.1578

A Preliminary Study on the Antigenic Proteins of Linguatula serrata Nymphal Stage in Sheep with Visceral Linguatulosis

Archives of Razi Institute
مقاله 5، دوره 77، شماره 1، فروردین و اردیبهشت 2022، صفحه 23-28    اصل مقاله (407.09 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.352744.1578
نویسندگان
Alireza Alborzi* 1؛ S Bahrami2؛ F Khajeh2
1Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran.
2Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده
Scant information is available on the immunological aspect of Linguatula serrata causing linguatulosis in humans and animals. The present study aimed to analyze the content of crude somatic extracts and excretory-secretory products of L. serrata nymphs to detect the immune response of sheep and immunogenic proteins of the parasite. After collecting the nymphs, somatic extracts were prepared by sonication. Excretory secretory products were prepared by the incubation of nymphs in RPMI medium at 37°C with 5% CO2. Somatic and excretory-secretory proteins were isolated using SDS-PAGE. The immunogenic properties of the resulting proteins were determined using immunoblotting and positive sera from sheep infected with visceral linguatulosis.  The total content of somatic extracts and excretory-secretory products of L. serrata nymphs analyzed by SDS-PAGE (12% gel) revealed two protein patterns with more than 18 and 9 strong bands, respectively.  Immunoblots using sera samples of sheep infected with the parasite, somatic extracts and excretory-secretory products demonstrated 12 and 3 antigenic proteins with molecular weights mostly in the range of 24-100 kDa and an antigen more than 180 kDa. Three common immunodominant antigenic proteins with molecular weights of 38 and 57, as well as an antigen of more than 180 kDa, were detected in the somatic extracts and excretory-secretory products of L. serrata nymphs in sheep with visceral linguatulosis. These antigens can be considetered prime candidates for future serodiagnosis and immunoprotective studies of the parasite.
کلیدواژه‌ها
Antigen؛ Immunoblotting؛ Linguatula serrata؛ Sheep
عنوان مقاله [English]
مطالعه اولیه پروتئین های آنتی ژنی مرحله نوچه ای لینگواتولا سراتا در گوسفندان مبتلا به لینگواتولوز احشائی
چکیده [English]
ندارد
کلیدواژه‌ها [English]
لینگواتولا سراتا, آنتی ژن, ایمونوبلاتینگ, گوسفند
سایر فایل های مرتبط با مقاله
مراجع
  1. Mehlhorn H. Encyclopedic Reference of Parasitology. 2nd ed: Heidelberg: Springer-Verlag; 2007.
  2. Soulsby EJL. Helminths, Arthropods and Protozoa of Domesticated Animals: Lea & Febiger; 1982.
  3. Acha P, Szyfres B. Pentastomiases. zoonosis and communicable diseases common to man and animals. 3. 3rd ed: PAHO; 2003. p. 345-50.
  4. Baird JK, Kassebaum LJ, Ludwig GK. Hepatic granuloma in a man from North America caused by a nymph of Linguatula serrata. Pathology. 1988;20(2):198-9.
  5. El-Hassan AM, Eltoum IA, El-Asha BMA. The Marrara syndrome: isolation of Linguatula serrata nymphs from a patient and the viscera of goats. Trans R Soc Trop Med Hyg. 1991;85(2):309-.
  6. Lazo RF, Hidalgo E, Lazo JE, Bermeo A, Llaguno M, Murillo J, et al. Ocular linguatuliasis in Ecuador: case report and morphometric study of the larva of Linguatula serrata. Am J Trop Med Hyg. 1999;60(3):405-9.
  7. Tappe D, Winzer R, Buttner DW, Strobel P, Stich A, Klinker H, et al. Linguatuliasis in Germany. Emerg Infect Dis. 2006;12(6):1034-6.
  8. Yagi H, El Bahari S, Mohamed HA, Ahmed ERS, Mustafa B, Mahmoud M, et al. The Marrara syndrome: a hypersensitivity reaction of the upper respiratory tract and buccopharyngeal mucosa to nymphs of Linguatula serrata. Acta Tropica. 1996;62(3):127-34.
  9. Lang Y, Garzozi H, Epstein Z, Barkay S, Gold D, Lengy J. Intraocular pentastomiasis causing unilateral glaucoma. Br J Ophthalmol. 1987;71(5):391-5.
  10. Riley J. The Biology of Pentastomids. In: Baker JR, Muller R, editors. Advances in Parasitology. 25: Academic Press; 1986. p. 45-128.
  11. Ma KC, Qiu MH, Rong YL. Pathological differentiation of suspected cases of pentastomiasis in China. Trop Med Int Health. 2002;7(2):166-77.
  12. Anaraki Mohammadi G, Mobedi I, Ariaiepour M, Pourmohammadi Z, Zare Bidaki M. A Case Report of Nasopharyngeal Linguatuliasis in Tehran, Iran and Characterization of the Isolated Linguatula serrata. Iran J Parasitol. 1970;3(1).
  13. Maleky F. A case report of Linguatula serrata in human throat from Tehran, central Iran. Indian journal of medical sciences. 2001;55(8):439-41.
  14. Sadjjadi S, Ardehali S, Shojaei A. A Case Report Of Linguatula Serrata In Human Pharynx From Shiraz, Southern Iran. Med J Islam Repub Iran. 1998;12(2):193-4.
  15. Siavashi M, Assmar M, Vatankhah A. Nasopharyngeal Pentastomiasis (Halzoun): Report of 3 Cases. Iran J Med Sci. 2015;27(4):191-2.
  16. Alborzi A, Molayan PH, Akbari M. Prevalence of Linguatula serrata Nymphs in Mesenteric Lymph Nodes of Cattle and Buffaloes Slaughtered in Ahvaz Abattoir, Iran. Iran J Parasitol. 2013;8(2):327-32.
  17. Tasan E. Distribution of Linguatula serrata (Frohlich, 1789) in dogs from rural districts of Elazığ. Doğa Vet Hay Derg. 1987;11:86-9.
  18. Oluwasina OS, Thankgod OE, Augustine OO, Gimba FI. Linguatula serrata (Porocephalida: Linguatulidae) Infection among Client-Owned Dogs in Jalingo, North Eastern Nigeria: Prevalence and Public Health Implications. J Parasitol Res. 2014;2014:916120.
  19. Alborzi A, Darakhshandeh T. A survey on infection of Linguatula serrata nymphs in sheep slaughtered at Yasuj abattoir. Iran J Vet Res. 2007;4:103-9.
  20. Esmaeilzadeh S, Mohammadian B, Rezaie A. Linguatula serrata nymph in a cat. Iran J Vet Res. 2008;9:387.
  21. Nourollahi Fard SR, Kheirandish R, Asl EN, Fathi S. Mesenteric and mediastinal lymph node infection with Linguatula serrata nymphs in sheep slaughtered in Kerman slaughterhouse, southeast Iran. Trop Anim Health Prod. 2011;43(1):1-3.
  1. Oryan A, Khordadmehr M, Ranjbar VR. Prevalence, biology, pathology, and public health importance of linguatulosis of camel in Iran. Trop Anim Health Prod. 2011;43(6):1225-31.
  2. Oryan A, Sadjjadi SM, Mehrabani D, Rezaei M. The status of Linguatula serrata infection of stray dogs in Shiraz, Iran. Comp Clin Path. 2008;17(1):55-60.
  3. Razavi SM, Shekarforoush SS, Izadi M. Prevalence of Linguatula serrata nymphs in goats in Shiraz, Iran. Small Rumin Res. 2004;54(3):213-7.
  4. Saiyari M, Mohammadian B, Sharma RN. Linguatula serrata (Frolich 1789) nymphs in lungs of goats in iran. Trop Anim Health Prod. 1996;28(4):312-4.
  5. Shekarforoush SS, Razavi SM, Izadi M. Prevalence of Linguatula serrata nymphs in sheep in Shiraz, Iran. Small Rumin Res. 2004;52(1):99-101.
  6. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
  7. Hajipour N, Tavassoli M, Tayefi-Nasrabadi H, Seyedi S. Comparative evaluation of somatic proteins of the nymphal stage of Linguatula serrata isolated from goats and cattle in northwest Iran. Bulg J Vet Med. 2016;19:242-6.
  8. Ogunremi O, Lankester M, Loran S, Gajadhar A. Evaluation of excretory-secretory products and somatic worm antigens for the serodiagnosis of experimental Parelaphostrongylus tenuis infection in white-tailed deer. J Vet Diagn Invest. 1999;11(6):515-21.
30.          Alborzi AR, Ghorbanpoor M, Hamidnejat H, Poormehdi Boroujeni M, Mehdizadeh A. Development And Evaluation Of An Indirect Elisa Test For Serodiagnosis Of Linguatula Serrata Infection In Sheep. Iran J Vet Res. 2015;11(1). 

آمار
تعداد مشاهده مقاله: 1,584
تعداد دریافت فایل اصل مقاله: 2,720


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب