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Al-Shaeli, S. J. J, Ethaeb, A. M, Al-Zaidi, E. A. N. (1401). Serological and Histological Evaluation of the Effect of Honeybee Venom on Pancreas and Liver in Diabetic Mice. سامانه مدیریت نشریات علمی, 77(3), 1125-1131. doi: 10.22092/ari.2022.357385.2025
S. J. J Al-Shaeli; A. M Ethaeb; E. A. N Al-Zaidi. "Serological and Histological Evaluation of the Effect of Honeybee Venom on Pancreas and Liver in Diabetic Mice". سامانه مدیریت نشریات علمی, 77, 3, 1401, 1125-1131. doi: 10.22092/ari.2022.357385.2025
Al-Shaeli, S. J. J, Ethaeb, A. M, Al-Zaidi, E. A. N. (1401). 'Serological and Histological Evaluation of the Effect of Honeybee Venom on Pancreas and Liver in Diabetic Mice', سامانه مدیریت نشریات علمی, 77(3), pp. 1125-1131. doi: 10.22092/ari.2022.357385.2025
Al-Shaeli, S. J. J, Ethaeb, A. M, Al-Zaidi, E. A. N. Serological and Histological Evaluation of the Effect of Honeybee Venom on Pancreas and Liver in Diabetic Mice. سامانه مدیریت نشریات علمی, 1401; 77(3): 1125-1131. doi: 10.22092/ari.2022.357385.2025

Serological and Histological Evaluation of the Effect of Honeybee Venom on Pancreas and Liver in Diabetic Mice

Archives of Razi Institute
مقاله 25، دوره 77، شماره 3، مرداد و شهریور 2022، صفحه 1125-1131    اصل مقاله (570.2 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357385.2025
نویسندگان
S. J. J Al-Shaeli* 1؛ A. M Ethaeb2؛ E. A. N Al-Zaidi3
1Department of Medical Basic Sciences, College of Dentistry, Wasit University, Wasit, Iraq
2Department of Anatomy and Histology, College of Veterinary Medicine, Wasit University, Wasit, Iraq
3Department of Public Health, College of Veterinary Medicine, Wasit University, Wasit, Iraq
چکیده
Natural toxins have been traditionally used to trigger several diseases among which bee venom (HBV) is of great importance. The present study aimed to investigate the therapeutic effects of honeybee venom (HBV) on alloxan and glucose fluid-induced Type 2 diabetes mellitus (T2DM). Therefore, a total of 20 adult laboratory male mice (Mus musculus) were selected, acclimated, and divided into four equal groups (n=5). Initially, 15 mice were fasted for 12 hrs and injected with alloxan at a single dose of 150 mg/kg of body weight. The animals were exposed to drinking glucose fluid in the morning for 4 days. Then, the blood glucose was measured. The studied animals having blood glucose of £200 mg/dl were considered non-diabetic and re-subjected to injecting alloxan (150 mg/kg body weight) and drinking glucose fluid for another 4 days. Four groups of mice population included, Group 1: non-diabetic and untreated with HBV, Group 2: diabetic and received no HBV as the potential therapeutic agent, Group 3: diabetic and received a low level of HBV at a dose of 0.5 mg/kg, Group 4: diabetic and received a high level of HBV at a dose of 1 mg/kg. At the end of the 35-day testing period, blood samples were tested to determine the levels of insulin, glucose, and lipid profiles [cholesterol, triglyceride (TG), high-density lipoprotein (HDL), and low-density lipoprotein (LDL)] using Sandwich ELISA kits. The results indicated a significant increase in blood glucose in the diabetic group compared to that of the control one, while both concentrations of HBV significantly reduced the level of blood glucose compared to that of the diabetic group. Furthermore, the level of insulin was significantly decreased in the diabetic group compared to that of the controls, while HBV significantly increased the level of insulin compared to that of the diabetic group. Moreover, the diabetic mice demonstrated a significant increase in the concentration of cholesterol and TG compared to that of control mice which were significantly reversed in response to HBV treatment. The level of HDL was significantly decreased in the diabetic group compared to that of the control group which was modulated by treatment, while no significant differences were seen between all the studied groups regarding the level of LDL. Histological examination of diabetic mice revealed a significant alteration in acinar cells and destruction of b-cells of pancreatic sections with marked lacerations in the liver extended to all structures of the organ. The present study concluded that HBV could be a potential therapeutic agent to prevent and manage diabetes and its complication.
کلیدواژه‌ها
Cholesterol؛ Diabetes Mellitus؛ Iraq؛ HDL؛ LDL؛ Triglyceride
مراجع
  1. Razaq RA, Mahdi JA, Jawad RA. Information about Diabetes Mellitus.J Univ Babylon eng sci. 2020:243-52.
  2. Cole JB, Florez JC. Genetics of diabetes mellitus and diabetes complications. Nat Rev Nephrol. 2020;16(7):377-90.
  3. Gu H, Han SM, Park K-K. Therapeutic effects of apamin as a bee venom component for non-neoplastic disease. Toxins. 2020;12(3):195.
  4. Phillips LS, Ratner RE, Buse JB, Kahn SE. We can change the natural history of type 2 diabetes. Diabetes care. 2014;37(10):2668-76.
  5. Skyler JS, Bakris GL, Bonifacio E, Darsow T, Eckel RH, Groop L, et al. Differentiation of diabetes by pathophysiology, natural history, and prognosis. Diabetes. 2017;66(2):241-55.
  6. Mousavi SM, Imani S, Haghighi S, Mousavi SE, Karimi A. Effect of Iranian honey bee (Apis mellifera) venom on blood glucose and insulin in diabetic rats. J Arthropod Borne Dis. 2012;6(2):136.
  7. Behroozi J, Divsalar A, Saboury AA. Honey bee venom decreases the complications of diabetes by preventing hemoglobin glycation. J Mol Liq. 2014;199:371-5.
  8. Jeong H, Lee C, Cheng C, Chou HC, Yang H, Bae H. Targeting of adipose tissue macrophages by bee venom phospholipase A2 attenuates high-fat diet-induced obesity. Int J Obes. 2021:1-12.
  9. Shakir AT, Mahdi RK, Al-Morshidy KA. A weak effects of bee venom on rat superoxide dismutase, catalase and Malondialdehyde activity: Rheumatoid arthritis model. EurAsian J Biosci. 2020;14(2):2983-7.
  10. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972;18(6):499-502.
  11. Knopfholz J, Disserol CCD, Pierin AJ, Schirr FL, Streisky L, Takito LL, et al. Validation of the friedewald formula in patients with metabolic syndrome. Cholesterol. 2014;2014.
  12. Al-Shaeli SJ, Ethaeb AM, Gharban HA. Molecular and histopathological identification of ovine neosporosis (Neospora caninum) in aborted ewes in Iraq. Vet World. 2020;13(3):597.
  13. Kleinert M, Clemmensen C, Hofmann SM, Moore MC, Renner S, Woods SC, et al. Animal models of obesity and diabetes mellitus. Nat Rev Endocrinol. 2018;14(3):140-62.
  14. Kader AA, Azmy R, Maher EA, El Sayed BB, Khalil AS, Ghalwash M, et al. Assessment of bee venom therapy in animal model of statin-induced myopathy. Egypt J Neurol Psychiatry Neurosurg. 2019;55(1):1-6.
  15. El Senosi YA, Omayma A, Elmaged ADA, Ali MA. Biochemical study on the regenerative effect of bee venom on experimentally induced diabetes. World J Pharm Pharm Sci. 2018;10:209-25.
  16. Elkotby D, Hassan AK, Emad R, Bahgat I. Histological changes in islets of Langerhans of pancreas in alloxan-induced diabetic rats following Egyptian honey bee venom treatments. Int J Pure Appl Zool. 2018;6(1):1-6.
  17. Fujimoto WY, METZ SA. Phasic effects of glucose, phospholipase A2, and lysophospholipids on insulin secretion. Endocrinology. 1987;120(5):1750-7.
  18. Fletcher JE, Jiang M-S. Possible mechanisms of action of cobra snake venom cardiotoxins and bee venom melittin. Toxicon. 1993;31(6):669-95.
  19. Hassan AK, El-kotby DA, Tawfik MM, Badr RE, Bahgat IM. Antidiabetic effect of the Egyptian honey bee (Apis mellifera) venom in alloxan-induced diabetic rats. J basic Appl Zool. 2019;80(1):1-9.
  1. Tsutsumi K, Hagi A, Inoue Y. The relationship between plasma high density lipoprotein cholesterol levels and cholesteryl ester transfer protein activity in six species of healthy experimental animals. Biol Pharm Bull. 2001;24(5):579-81.
  2. Ivas C, Solcan G, Pavel G, Burcă I, Pavel M. Glycemia and lipidemia variations of the rabbits inoculated with bee venom. USAMV, Romania. 2011.
  3. Singh J, Ranganathan R. Quantitation of lysolipids, fatty acids, and phospholipase A2 activity and correlation with membrane polarity. J Lipid Res. 2012;53(9):1993-2001.
  4. M K. Effect of Honey Bee Venom (Apis mellifera) on Hyperglycemia and Hyperlipidemia in Alloxan Induced Diabetic Rabbits. Journal of Diabetes & Metabolism. 2015;06.
  5. Nurdiana S, Goh YM, Ahmad H, Dom SM, Azmi NSa, Zin NSNM, et al. Changes in pancreatic histology, insulin secretion and oxidative status in diabetic rats following treatment with Ficus deltoidea and vitexin. BMC Complement Med ther. 2017;17(1):1-17.
  6. Bilal HM, Riaz F, Munir K, Saqib A, Sarwar MR. Histological changes in the liver of diabetic rats: A review of pathogenesis of nonalcoholic fatty liver disease in type 1 diabetes mellitus. Cogent Med. 2016;3(1):1275415.
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