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Hussain, E. A, Qasim Hameed, H, Mujahid Al-Shuwaikh, A, Mujahid Abdullah, R. (1401). Detection of the aadA1 and aac (3)-1V resistance genes in Acinetobacter baumannii. سامانه مدیریت نشریات علمی, 77(3), 959-966. doi: 10.22092/ari.2022.357271.2010
E. A Hussain; H Qasim Hameed; A Mujahid Al-Shuwaikh; R Mujahid Abdullah. "Detection of the aadA1 and aac (3)-1V resistance genes in Acinetobacter baumannii". سامانه مدیریت نشریات علمی, 77, 3, 1401, 959-966. doi: 10.22092/ari.2022.357271.2010
Hussain, E. A, Qasim Hameed, H, Mujahid Al-Shuwaikh, A, Mujahid Abdullah, R. (1401). 'Detection of the aadA1 and aac (3)-1V resistance genes in Acinetobacter baumannii', سامانه مدیریت نشریات علمی, 77(3), pp. 959-966. doi: 10.22092/ari.2022.357271.2010
Hussain, E. A, Qasim Hameed, H, Mujahid Al-Shuwaikh, A, Mujahid Abdullah, R. Detection of the aadA1 and aac (3)-1V resistance genes in Acinetobacter baumannii. سامانه مدیریت نشریات علمی, 1401; 77(3): 959-966. doi: 10.22092/ari.2022.357271.2010

Detection of the aadA1 and aac (3)-1V resistance genes in Acinetobacter baumannii

Archives of Razi Institute
مقاله 5، دوره 77، شماره 3، مرداد و شهریور 2022، صفحه 959-966    اصل مقاله (733.89 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357271.2010
نویسندگان
E. A Hussain1؛ H Qasim Hameed* 1؛ A Mujahid Al-Shuwaikh2؛ R Mujahid Abdullah1
1Department of Biology, College of Education for Pure Science Ibn-Al Haitham, University of Baghdad, Baghdad, Iraq
2Microbiology Department, College of Medicine, Al-Nahrain University, Baghdad, Iraq
چکیده
Acinetobacter baumannii is a gram-negative aerobic bacterium that can be found in different environments, such as food, containing vegetables, meat, and fish; moreover, it can be present in soil and freshwater. A. baumannii has globally considered an opportunistic nosocomial bacterium in the healthcare setting contributing to increased morbidity and mortality. The current study aimed to detect the aminoglycoside genes in A. baumannii isolated from different clinical causes. In total, 20 isolates of A. baumannii were obtained from different clinical cases. Bacterial isolate DNA was extracted using a DNA extraction kit. Quantus Fluorometer was used to detect the concentration of the extracted DNA in order to detect the goodness of samples. 1 μl of DNA and 199 μl of diluted QuantiFlour Dye were mixed. After 5 min incubation at room temperature, DNA concentration values were evaluated, and following the initial amplification of the A. baumannii aada1 gene, 20 μl of PCR product with F and R primers were sent to Sanger sequencing. The results of the antimicrobial susceptibility revealed that A. baumannii isolates were resistant to Gentamicin (95%), Amikacin (90%), and Tobramycin (60%). Molecular investigation of the aadA1 and aac (3)-IV genes exhibited that the aadA1 gene was detected in 15% of the isolates. However, the aac (3)-IV gene was not detected in any of the isolates. The gel electrophoresis revealed that the molecular weight of the aadA1 gene was 490bp. The DNA sequence of the aadA1 gene was conducted in this study, and the results exhibited no mutations in all isolates.
کلیدواژه‌ها
aac (3)-1V؛ Acinetobacter baumannii؛ aadA1؛ Resistance genes in bacteria
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