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Adnan Khalaf, M, Hasan Al-Saadi, B. Q, Qassim Mohammed, H, Hussein Ewaid, S. (1401). Alpha-Hemoglobin Stabilizing Protein Gene Polymorphism (rs4499252 A/G) and its Association with Beta-Thalassemia Major in Iraqi Patients. سامانه مدیریت نشریات علمی, 77(3), 1033-1039. doi: 10.22092/ari.2022.357268.2007
M Adnan Khalaf; B. Q Hasan Al-Saadi; H Qassim Mohammed; S Hussein Ewaid. "Alpha-Hemoglobin Stabilizing Protein Gene Polymorphism (rs4499252 A/G) and its Association with Beta-Thalassemia Major in Iraqi Patients". سامانه مدیریت نشریات علمی, 77, 3, 1401, 1033-1039. doi: 10.22092/ari.2022.357268.2007
Adnan Khalaf, M, Hasan Al-Saadi, B. Q, Qassim Mohammed, H, Hussein Ewaid, S. (1401). 'Alpha-Hemoglobin Stabilizing Protein Gene Polymorphism (rs4499252 A/G) and its Association with Beta-Thalassemia Major in Iraqi Patients', سامانه مدیریت نشریات علمی, 77(3), pp. 1033-1039. doi: 10.22092/ari.2022.357268.2007
Adnan Khalaf, M, Hasan Al-Saadi, B. Q, Qassim Mohammed, H, Hussein Ewaid, S. Alpha-Hemoglobin Stabilizing Protein Gene Polymorphism (rs4499252 A/G) and its Association with Beta-Thalassemia Major in Iraqi Patients. سامانه مدیریت نشریات علمی, 1401; 77(3): 1033-1039. doi: 10.22092/ari.2022.357268.2007

Alpha-Hemoglobin Stabilizing Protein Gene Polymorphism (rs4499252 A/G) and its Association with Beta-Thalassemia Major in Iraqi Patients

Archives of Razi Institute
مقاله 14، دوره 77، شماره 3، مرداد و شهریور 2022، صفحه 1033-1039    اصل مقاله (543.21 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357268.2007
نویسندگان
M Adnan Khalaf* 1؛ B. Q Hasan Al-Saadi1؛ H Qassim Mohammed2؛ S Hussein Ewaid3
1Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Baghdad, Iraq
2College of Medicine, University of Wasit, Wasit, Iraq
3Shatra Technical College, Southern Technical University, Basra, Iraq
چکیده
Beta thalassemia (β-thalassemia) major is a genetic disorder of hemoglobin production that results in a diminished rate of synthesis of one or more of the globin chains causing variable degrees of anemia. Alpha-hemoglobin-stabilizing protein (AHSP) is a specific alpha-globin factor that affects the severity of the disease in patients with β-thalassemia. A recent study was conducted to investigate the polymorphism in the AHSP (rs4499252) gene and its association with β-thalassemia in Iraq. Blood samples were obtained from 90 β-thalassemia patients and 60 healthy individuals as a control group in the Wasit Center for Hereditary Anemia from August 2020 to January 2021. After DNA extraction from the whole blood, to determine the genotype of the AHSP gene, the High-Resolution Melt (HRM) Real-Time PCR was used. The results showed a significant increase (P<0.05) in genotype GG (wild type) of the SNP (rs4499252) in β-thalassemia patients, compared to the control group. On the other hand, genotype AA was significantly higher (P≤0.05) in β-thalassemia patients than in the control group, while the genotype GA showed a non-significant difference (P<0.01) between β-thalassemia patients and the healthy controls. The results also showed that the AHSP expression is a biomarker of hemoglobin H disease severity, and the A allele was more frequent in β-thalassemia patients than the G allele in Iraqi patients.
کلیدواژه‌ها
AHSP؛ β-thalassemia؛ Iraq؛ Polymorphism؛ Real-Time PCR
مراجع
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