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تنوع سوماکلونال در گیاهان باززایی‌شده لیلکی (.Gleditschia caspica Desf) با استفاده از نشانگرهای بین ریزماهواره ژنومی (ISSR-PCR) | ||
| تحقیقات جنگل و صنوبر ایران | ||
| مقاله 3، دوره 29، شماره 4 - شماره پیاپی 86، دی 1400، صفحه 326-338 اصل مقاله (327.99 K) | ||
| نوع مقاله: علمی- پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijfpr.2022.356617.2027 | ||
| نویسندگان | ||
| مجتبی ایمانی راستابی* 1؛ سید محمد حسینی نصر2؛ غلامعلی رنجبر3؛ مصطفی خوشحال سرمست4 | ||
| 1نویسنده مسئول، دانش‌آموخته دکتری، گروه علوم و مهندسی جنگل، دانشکده منابع طبیعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران | ||
| 2دانشیار، گروه علوم و مهندسی جنگل، دانشکده منابع طبیعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران | ||
| 3دانشیار، گروه اصلاح نباتات، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران | ||
| 4استادیار، گروه علوم باغبانی، دانشکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
| چکیده | ||
| تنوع سوماکلونال، آزمایش ژنتیکی است که با استفاده از آن می‌توان تغییرات ژنی و ثبات ژنتیکی گیاه تکثیرشده را نسبت به گیاه مادری ارزیابی کرد. هدف این پژوهش، بررسی تنوع سوماکلونال در گیاهان باززایی‌شده لیلکی (Gleditsia caspica Desf.) تحت تأثیر تنظیم‌کننده رشد BAP و بازدارنده رشد CPZ بود. پس از بذرگیری از یک درخت لیلکی در باغ گیاه‌شناسی دانشکده منابع طبیعی در دانشگاه علوم کشاورزی و منابع طبیعی ساری، ژنوتیپ‌های پایه مادری و گیاهان باززایی‌شده در محیط‌های کشت حاوی BAP (20 میکرومول) و CPZ (3/0، سه و 15 میکرومول) با استفاده از نشانگر مولکولی ISSR مقایسه شدند. بیشترین درصد شاخه‌زایی و تعداد شاخساره در تیمار BAP با غلظت 20 میکرومول مشاهده شد. وجود بازدارنده رشد CPZ در محیط کشت به‌شدت سبب کاهش درصد شاخه‌زایی ریزنمونه‌ها شد. همچنین، روند کاهشی مشخصه‌های مورد مطالعه با افزایش غلظت CPZ تشدید یافت. استفاده از 10 نشانگر مولکولی ISSR درمجموع، 107 نوار DNA تولید کرد که 9/87 درصد آن‌ها، چندشکلی بودند. دارنگاره تجزیه خوشه‌‌ای براساس ماتریس تشابه جاکارد به‌روش UPGMA نشان داد که ژنوتیپ‌های مورد مطالعه با ضریب تشابه 27/0 در دو گروه قرار گرفتند. به‌نظر می‌رسد که وجود بازدارنده رشد CPZ به‌‌رغم اثر منفی بر رویش گیاه سبب افزایش تنوع و کاهش شباهت ژنتیکی بین گیاهان باززایی‌شده و پایه مادری می‌شود. | ||
| کلیدواژه‌ها | ||
| بازدارنده رشد؛ تنظیم‌کننده رشد؛ تنوع ژنتیکی؛ کشت بافت | ||
| عنوان مقاله [English] | ||
| Somaclonal diversity in Caspian honey locust (Gleditsia caspica Desf.) regenerated plants using genomic microsatellite markers (ISSR-PCR) | ||
| نویسندگان [English] | ||
| M. Imani Rastabi1؛ S.M. Hosseini-Nasr2؛ Gh. Ranjbar3؛ M. Khoshhal Sarmast4 | ||
| 1Corresponding author, Ph.D. Graduated, Department of Forestry Science and Engineering, Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources University, Sari, Iran | ||
| 2Associate Prof., Department of Forestry Science and Engineering, Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources University, Sari, Iran | ||
| 3Associate Prof., Department of Plant Breeding and Biotechnology, Sari Agricultural sciences and Natural Resources University, Sari, Iran | ||
| 4Assistant Prof., Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| چکیده [English] | ||
| Somaclonal diversity is a genetical test that can be used to study the genetic variation and genetic stability of a propagated plant relative to the mother plant. The aim of this study was to investigate the genetic stability of Caspian honey locust (Gleditsia caspica Desf.) regenerated plants under the influence of BAP growth regulator and CPZ growth inhibitor. For this purpose, native and regenerated plants genotypes were compared in culture media containing growth regulator BAP (20 μmol) and CPZ (0.3, 3 and 15 μmol) used by molecular marker of ISSR. The highest percentage of shoot proliferation and number of shoots was observed in 20 μmol BAP treatment. The presence of CPZ in the culture medium greatly reduced the shoot proliferation percentage and with increasing CPZ concentration, the decreasing trend of the studied characteristics increased. The results showed that the use of 10 ISSR molecular markers produced a total of 107 DNA bands that had 87.9% polymorphism. Cluster analysis dendrogram based on Jaccard similarity matrix by UPGMA method showed that the studied genotypes were divided into two groups with similarity coefficient of 0.27. It seems that the presence of CPZ, despite the growth inhibitory effect on the plant, will increase diversity and reduce the genetic affinity between the regenerated plants and the mother plant. | ||
| کلیدواژه‌ها [English] | ||
| Genetic diversity, growth inhibitor, growth regulator, tissue culture | ||
| مراجع | ||
|
- Akdemir, H., Suzerer, V., Tilkat, E., Onay, A. and Ciftci, Y.O., 2016. Detection of variation in long-term micropropagated mature pistachio via DNA-based molecular markers. Applied Biochemistry and Biotechnology, 180(7): 1301-1312. - Ando, T., Akiyama, Y. and Yokoi, M., 1986. Flower colour sports in saintpaulia cultivars. Scientia Horticulturae, 29(1-2): 191-197. - Ayadi, R., Hamrouni, L., Hanana, M., Bouzid, S., Trifi, M. and Khouja, M.L., 2011. In vitro propagation and regeneration of an industrial plant kenaf (Hibiscus cannabinus L.). Industrial Crops and Products, 33(2): 474-480. - Azman, A.S., Mhiri, C., Grandbastien, M.A. and Tam, S., 2014. Transposable elements and the detection of somaclonal variation in plant tissue culture: a review. Malaysian Applied Biology, 43(1): 1-12. - Bagga, S., Das, R. and Sopory, S.K., 1987. Inhibition of cell proliferation and glyoxalase-I activity by calmodulin inhibitors and lithium in Brassica oleracea. Journal of Plant Physiology, 129(1-2):149-153. - Bagheri, A. and Saffari, M., 1997. In Vitro Culture of Higher Plants (translation). Ferdowsi University of Mashhad Press, Mashhad, 406p (In Persian). - Bairu, M.W., Aremu, A.O. and Van Staden, J., 2011. Somaclonal variation in plants: causes and detection methods. Plant Growth Regulation, 63(2): 147-173. - Bianco-Colomas, J., 1983. Effect of a cytokinin antagonist on cytokinin and light-dependent amaranthin synthesis in Amaranthus tricolor seedlings. Journal of Plant Growth Regulation, 2(1-4): 281. - Buchanan, F.C. and Thue, T.D., 1998. Intrabreed polymorphic information content of microsatellites in cattle and sheep. Canadian journal of animal science, 78(3): 425-428. - D'amato, F., 1978. Chromosome number variation in cultured cells and regenerated plants: 287-295. In: Thorpe, T.A. (Ed.). Frontiers of Plant Tissue Culture. International Association for Plant Tissue Culture, Alberta, 556p. - Geburek, T., 1997. Isozymes and DNA markers in gene conservation of forest trees. Biodiversity and Conservation, 6(12): 1639-1654. - Ghandehari, V., Ahmadikhah, A. and Payamnoor, V., 2013. Genetic diversity of Buxus hyrcana populations in north of Iran using ISSR markers. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 21(1): 1-12 (In Persian). - Hamrick, J.L. and Godt, M.J.W., 1996. Conservation genetics of endemic plant species: 281-304. In: Avise, J.C. and Hamrick, J.L. (Eds.). Conservation Genetics: Case Histories from Nature. Chapman and Hall, New York, 512p. - Jain, S.M., 2001. Tissue culture-derived variation in crop improvement. Euphytica, 118(2): 153-166. - Karanov, E., Alexieva, V. and Sergiev. I., 1992. Cytokinin antagonists-chemistry and action. Bulgarian Journal of Plant Physiology, 1: 97-107. - Khawale, R.N., Singh, S.K., Vimala, Y., Minakshi, G., 2006. Assessment of clonal fidelity of micropropagated grape (Vitis vinifera L.) plants by RAPD analysis. Physiology and Molecular Biology of Plants, 12(2): 189-192. - Khosh-Khui, M., 1998. Tissue Culture Techniques for Horticultural Crops (translation). Shiraz University Press, Shiraz, 438p (In Persian). - Krishna, H., Alizadeh, M., Singh, D., Singh, U., Chauhan, N., Eftekhari, M. and Sadh, R.K. 2016. Somaclonal variations and their applications in horticultural crops improvement. 3 Biotech, 6(1): 54. - Larkin, P.J. and Scowcroft, W.R., 1981. Somaclonal variation - a novel source of variability from cell cultures for plant improvement. Theoretical and Applied Genetics, 60(4): 197-214. - Lazaro-Castellanos, J.O., Mata-Rosas, M., González, D., Arias, S. and Reverchon, F., 2018. In vitro propagation of endangered Mammillaria genus (Cactaceae) species and genetic stability assessment using SSR markers. In Vitro Cellular and Developmental Biology - Plant, 54(5): 518-529. - Murray, M.G. and Thompson, W.F., 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8(19): 4321-4325. - Pastelín Solano, M.C., Salinas Ruíz, J., González Arnao, M.T., Castro, O.C., Galindo Tovar, M.E. and Bello Bello, J.J., 2019. Evaluation of in vitro shoot multiplication and ISSR marker based assessment of somaclonal variants at different subcultures of vanilla (Vanilla planifolia Jacks). Physiology and Molecular Biology of Plants, 25(2): 561-567. - Peng, X., Zhang, T.T. and Zhang, J., 2015. Effect of subculture times on genetic fidelity, endogenous hormone level and pharmaceutical potential of Tetrastigma hemsleyanum callus. Plant Cell, Tissue and Organ Culture, 122(1): 67-77. - Rahmani, M.Sh., Shabanian, N., Khadivi-Khub, A., Woeste, K.E., Badakhshan, H. and Alikhani, L., 2015. Population structure and genotypic variation of Crataegus pontica inferred by molecular markers. Gene, 572(1): 123-129. - Saito, H., Mizunashi, K., Tanaka, S., Adachi, Y. and Nakano, M., 2003. Ploidy estimation in Hemerocallis species and cultivars by flow cytometry. Science Horticulture, 97(2): 185-192. - Sarmast, M.K., 2016. Genetic transformation and somaclonal variation in conifers. Plant Biotechnology Reports, 10(6): 309-325. - Sarmast, M.K., Salehi, H. and Khosh-Khui, M., 2011. Nano silver treatment is effective in reducing bacterial contaminations of Araucaria excelsa R. Br. var. glauca explants. Acta Biologica Hungarica, 62(4): 477-484. - Shabanian, N., Havasi, A. and Mehrabi, A.A., 2016. Genetic differentiation in Persian oak (Quercus brantii) populations using genomic inter-microsatellite markers. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24(1): 66-78 (In Persian). - Shannon, C.W. and Weaver, W., 1949. The Mathematical Theory of Communication. University of Illinois Press, Urbana, 131p. - Sharma, S.K., Knox, M.R. and Ellis, T.H.N., 1996. AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis. Theoretical and Applied Genetics, 93(5-6): 751-758. - Singh, R., Kashyap, S.P., Kumari, N. and Singh, M., 2016. Regeneration of soapnut tree through somatic embryogenesis and assessment of genetic fidelity through ISSR and RAPD markers. Physiology and Molecular Biology of Plants, 22: 381-389. - Yanosaka, K., Iwamura, H. and Fujita, T., 1989. Flower induction in seedlings of Asparagus officinalis L. by N-phenylcarbamates. Zeitschrift für Naturforschung C, 44(3-4): 226-232. - Zhao, Y. and Hasenstein, K.H., 2010. Physiological interactions of antiauxins with auxin in roots. Journal of Plant Physiology, 167(11): 879-884. | ||
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