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Mohammed Jassim, Z, Mohammed Obead, F, Neama, S. (1401). Integron-Associated Antibiotic Resistance in Salmonella typhi. سامانه مدیریت نشریات علمی, 77(2), 771-777. doi: 10.22092/ari.2021.356953.1944
Z Mohammed Jassim; F Mohammed Obead; S Neama. "Integron-Associated Antibiotic Resistance in Salmonella typhi". سامانه مدیریت نشریات علمی, 77, 2, 1401, 771-777. doi: 10.22092/ari.2021.356953.1944
Mohammed Jassim, Z, Mohammed Obead, F, Neama, S. (1401). 'Integron-Associated Antibiotic Resistance in Salmonella typhi', سامانه مدیریت نشریات علمی, 77(2), pp. 771-777. doi: 10.22092/ari.2021.356953.1944
Mohammed Jassim, Z, Mohammed Obead, F, Neama, S. Integron-Associated Antibiotic Resistance in Salmonella typhi. سامانه مدیریت نشریات علمی, 1401; 77(2): 771-777. doi: 10.22092/ari.2021.356953.1944

Integron-Associated Antibiotic Resistance in Salmonella typhi

Archives of Razi Institute
مقاله 34، دوره 77، شماره 2، خرداد و تیر 2022، صفحه 771-777    اصل مقاله (524.49 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356953.1944
نویسندگان
Z Mohammed Jassim* 1؛ F Mohammed Obead2؛ S Neama2
1College of biotechnology, Al-Qasim Green University, Al Qasim, Iraq
2Department of Medical Laboratory Techniques, Faculty of Medical and Health Techniques, University of Alkafeel, Najaf, Iraq
چکیده
Salmonella enteric serovar Typhi (S. typhi) and paratyphi (S. paratyphi) bacteria exclusively found in humans, cause typhoid fever, an acute, and possibly deadly systemic infection. Typhoid fever is caused by a species of rod-shaped, Gram-negative Enterobacteriaceae called S. typhi. The present study aimed to examine the intI gene and investigate the possible relation between this gene and multi-drug resistance in S. typhi. A total of 30 blood samples were obtained from patients who were suspicious of typhoid fever using the direct strategy of inoculation. Each specimen was injected into a culture of a selective medium, such as XLD and SS agar, and then incubated at 37°C for 24 h. The genomic DNA was extracted through a boiling process. Tris-EDTA was used to suspend bacterial colonies cultured on MacConkey agar plates. The suspension of bacterial colonies was centrifuged for 5 min at 8000×g and for 20 min at -20°C which lyses the organisms and extracts the DNA from the buffer. The supernatant is then transferred to a fresh Eppendorf tube. Gel electrophoresis was carried out utilizing a UV transilluminator. The intI gene for S. typhi was found using a PCR test. The antibiotic sensitivity testing showed that the S. typhi isolates were classed as multi-resistant. These results were confirmed using the polymerase chain reaction (PCR) technique using intI gene where twenty specimens isolated from typhoid patients were positive for S. typhi.
کلیدواژه‌ها
Integron gene؛ Multidrug resistance؛ S. typhi
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مراجع
  1. Capoor MR, Nair D. Quinolone and cephalosporin resistance in enteric fever. J Glob Infect Dis. 2010;2(3):258.
  2. Kelly A, Goldberg MD, Carroll RK, Danino V, Hinton JC, Dorman CJ. A global role for Fis in the transcriptional control of metabolism and type III secretion in Salmonella enterica serovar Typhimurium. Microbiology. 2004;150(7):2037-53.
  3. Debdas D, Joshi S. Mixed Salmonella infection. Indian journal of medical microbiology. 2008;26(3):287-.
  4. Al-Muhanna SG, Banoon SR, Al-Kraety IAA. Molecular detection of integron class 1 gene in proteus mirabilis isolated from diabetic foot infections. Plant Arch. 2020;2(1):3101-7.
  5. Mazel D. Integrons: agents of bacterial evolution. Nat Rev Microbiol. 2006;4(8):608-20.
  6. Cheesbrough M. District Laboratory Practice in Tropical Countries. Part 1. Ed2. 2009.
  7. Cheng Z, De Jesus OP, Namavari M, De A, Levi J, Webster JM, et al. Small-animal PET imaging of human epidermal growth factor receptor type 2 expression with site-specific 18F-labeled protein scaffold molecules. J Nucl Med. 2008;49(5):804-13.
  8. Rosser SJ, Young H-KJJoAC. Identification and characterization of class 1 integrons in bacteria from an aquatic environment. J Antimicrob Chemother. 1999;44(1):11-8.
  9. Nalbantsoy A. Prevention and identification of Salmonella Enteritidis infection via novel diagnostic stained antigens and polyclonal antibodies. Food Agric Immunol. 2013;24(2):139-49.
  10. Cortez A, Carvalho A, Ikuno A, Bürger K, Vidal-Martins A. Resistência antimicrobiana de cepas de Salmonella spp. isoladas de abatedouros de aves. Arq Inst Biol. 2006;73(2):157-63.
  11. Bacci C, Boni E, Alpigiani I, Lanzoni E, Bonardi S, Brindani F. Phenotypic and genotypic features of antibiotic resistance in Salmonella enterica isolated from chicken meat and chicken and quail carcasses. Int J Food Microbiol. 2012;160(1):16-23.
  12. Galdino VMCA, de MELO RT, Oliveira RP, Mendonça EP, Nalevaiko PC, Rossi DA. Virulência de Salmonella spp. de origem avícola e resistência a antimicrobianos. Biosci J. 2013;29(4).
  13. Yoke‐Kqueen C, Learn‐Han L, Noorzaleha A, Son R, Sabrina S, Jiun‐Horng S, et al. Characterization of multiple‐antimicrobial‐resistant Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry in Malaysia. Lett Appl Microbiol. 2008;46(3):318-24.
  14. WHO. Report of the 6th meeting of the WHO advisory group on integrated surveillance of antimicrobial resistance with AGISAR 5-year strategic framework to support implementation of the global action plan on antimicrobial resistance (2015-2019), 10-12 June 2015, Seoul, Republic of Korea. 2015.
  15. Lutterloh E, Likaka A, Sejvar J, Manda R, Naiene J, Monroe SS, et al. Multidrug-resistant typhoid fever with neurologic findings on the Malawi-Mozambique border. Clin Infect Dis. 2012;54(8):1100-6.
  16. Akinyemi K, Smith S, Oyefolu AB, Coker A. Multidrug resistance in Salmonella enterica serovar typhi isolated from patients with typhoid fever complications in Lagos, Nigeria. Public Health. 2005;119(4):321-7.
  17. Kariuki S, Revathi G, Kiiru J, Mengo DM, Mwituria J, Muyodi J, et al. Typhoid in Kenya is associated with a dominant multidrug-resistant Salmonella enterica serovar Typhi haplotype that is also widespread in Southeast Asia. J Clin Microbiol. 2010;48(6):2171-6.
  18. Thong K-L, Bhutta ZA, Pang T. Multidrug-resistant strains of Salmonella enterica serotype typhi are genetically homogenous and coexist with antibiotic-sensitive strains as distinct, independent clones. Int J Infect Dis. 2000;4(4):194-7.
  19. Lee GY, Song J. Complete Genome Sequence of Salmonella enterica Serovar Typhi Strain ISP2825. Microbiol Resour Announc. 2021;10(41):00804-21.
  20. Baquero F, Tedim A-SP, Coque TM. Antibiotic resistance shaping multi-level population biology of bacteria. Front Microbiol. 2013;4:15.
  21. AL-Kraety IAA, Al-Ammar M. relation of class1 integron gene with multi-drug resistance salmonella typi isolates. Pak J Biotechnol Vol. 2017;14(4):537-41.
  22. Jones ME, Peters E. Widespread occurrence of integrons causing multiple antibiotic resistance in bacteria. Lancet. 1997;349(9067):1742-3.
  23. Al-Sanouri TM, Paglietti B, Haddadin A, Murgia M, Bacciu D, Youssef M, et al. Emergence of plasmid-mediated multidrug resistance in epidemic and non-epidemic strains of Salmonella enterica serotype Typhi from Jordan. J Infect Dev Ctries. 2008;2(04):295-301.
  24. Bashir S, Haque A, Sarwar Y, Raza A. Prevalence of integrons and antibiotic resistance amon. Arch Clin Microbiol. 2015;6(4).
  25. Ajiboye RM, Solberg OD, Lee BM, Raphael E, DebRoy C, Riley LW. Global spread of mobile antimicrobial drug resistance determinants in human and animal Escherichia coli and Salmonella strains causing community-acquired infections. Clin Infect Dis. 2009;49(3):365-71.
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