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The evolutionary relationship and the sequence similarities between different fungal species that infect birds captured from different areas in Denmark | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 28 آذر 1400 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2021.356858.1929 | ||
| نویسندگان | ||
Moayad Salh Hussin  ؛ Sinan Baho؛ Zaid AL-RUBAIEE
| ||
| Biological Department, College of Science, Mustansiriya University, Baghdad, Iraq | ||
| چکیده | ||
| Fungal disease are common causes of death in the wild animals and birds of prey. This study was designed to investigate the development of fungal infections in wild birds in Denmark. In this study, fungal samples were isolated from the following sources: Barn swallows' feather, White stork, and birds of prey. The fungal species were isolated by direct culture of feathers on SD Agar with chloramphenicol and incubated at 28º C ± 2. The fungal genomic DNA was isolated from each species and PCR reaction was performed and the resulting fragments of 18S rRNA DNA were sequenced and used for identification. A comparison between the resulting fragments were done to find out the percentage of similarity between the different fungal species. The multiple sequence alignment showed percentages of similarities ranged from 39% to 99%. To sum up, the18S rRNA DNA sequence has been evolved dramatically even within the same species, while still conserved in others. The DNA sequence of the 18S rRNA has been evolved dramatically even within the same species, while still conserved in others. It is useful tool to be used for identification of fungal species as it reduces the time. Moreover, from the results there were no comprehensive high homology percentage between species infecting the same bird. | ||
| کلیدواژه‌ها | ||
| evolutionary؛ sequence؛ similarities؛ Denmark | ||
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آمار تعداد مشاهده مقاله: 4 |
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