اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,846
تعداد مقالات32,300
تعداد مشاهده مقاله41,387,040
تعداد دریافت فایل اصل مقاله18,562,156
Yahya Allawi, M, Salim Al-Taie, B, Hmoshi, R. M. (1400). Isolation and Identification of Penicillium rubens from the Local Strain in Mosul, Iraq, and Investigation of Potassium Phosphate Effect on its Growth. سامانه مدیریت نشریات علمی, 77(1), 421-427. doi: 10.22092/ari.2021.356684.1896
M Yahya Allawi; B Salim Al-Taie; R. M Hmoshi. "Isolation and Identification of Penicillium rubens from the Local Strain in Mosul, Iraq, and Investigation of Potassium Phosphate Effect on its Growth". سامانه مدیریت نشریات علمی, 77, 1, 1400, 421-427. doi: 10.22092/ari.2021.356684.1896
Yahya Allawi, M, Salim Al-Taie, B, Hmoshi, R. M. (1400). 'Isolation and Identification of Penicillium rubens from the Local Strain in Mosul, Iraq, and Investigation of Potassium Phosphate Effect on its Growth', سامانه مدیریت نشریات علمی, 77(1), pp. 421-427. doi: 10.22092/ari.2021.356684.1896
Yahya Allawi, M, Salim Al-Taie, B, Hmoshi, R. M. Isolation and Identification of Penicillium rubens from the Local Strain in Mosul, Iraq, and Investigation of Potassium Phosphate Effect on its Growth. سامانه مدیریت نشریات علمی, 1400; 77(1): 421-427. doi: 10.22092/ari.2021.356684.1896

Isolation and Identification of Penicillium rubens from the Local Strain in Mosul, Iraq, and Investigation of Potassium Phosphate Effect on its Growth

Archives of Razi Institute
مقاله 52، دوره 77، شماره 1، فروردین و اردیبهشت 2022، صفحه 421-427    اصل مقاله (519.1 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356684.1896
نویسندگان
M Yahya Allawi* ؛ B Salim Al-Taie؛ R. M Hmoshi
Environmental Science Department, College of Environmental Science and Technologies, University of Mosul, Mosul, Iraq
چکیده
Penicillium species is one of the most common microscopic filamentous fungi that have been isolated from widespread substrates. In this study, soil samples from different areas of Mosul, Iraq, were examined by morphological and molecular methods. Fungi were isolated and grown in diagnostic culture media, including Czapek Yeast Extract Agar, Malt Extract Agar, and 25% Glycerol nitrate agar at different temperatures of 15, 20, 25, and 30°C. The growth rate of the fungus was also evaluated in the culture containing K2HPO4. In the molecular method, the obtained fragment was sequenced using the Internal Transcribed Spacer region primers after replication. Morphological evaluation of both macroscopic and microscopic features revealed that strains were Penicillium rubens, and molecular methods used have confirmed the isolated strain. The results of this study showed a decrease in the diameters of the colonies at different growth temperatures when doubling the amount of K2HPO4. In addition, the findings revealed that compared to growth inhibition in the control groups, the maximum growth inhibition was 16 and 29 mm on Czapek Dox Agar (modified) medium at incubation temperatures of 20°C and 25°C, respectively. As a result, K2HPO4 has an important role in inhibiting growth; therefore, it can be used as Fungicide.
کلیدواژه‌ها
ITS regions؛ K2HPO4؛ Penicillium rubens؛ Morphological characters
سایر فایل های مرتبط با مقاله
مراجع
  1. Abdel-Azeem AM, Abo Nahas HH, Abdel-Azeem MA, Tariq FJ, Yadav AN. Biodiversity and Ecological Perspective of Industrially Important Fungi An Introduction. In: Abdel-Azeem AM, Yadav AN, Yadav N, Usmani Z, editors. Industrially Important Fungi for Sustainable Development: Volume 1: Biodiversity and Ecological Perspectives. Cham: Springer International Publishing; 2021. p. 1-34.
  2. Houbraken J, Samson RA. Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Stud Mycol. 2011;70:1-51.
  3. Houbraken J, de Vries RP, Samson RA. Chapter Four - Modern Taxonomy of Biotechnologically Important Aspergillus and Penicillium Species. In: Sariaslani S, Gadd GM, editors. Advances in Applied Microbiology. 86: Academic Press; 2014. p. 199-249.
  4. Park MS, Oh S-Y, Fong JJ, Houbraken J, Lim YW. The diversity and ecological roles of Penicillium in intertidal zones. Sci Rep. 2019;9(1):13540.
  5. Barcus AL, Burdette SD, Herchline TE. Intestinal invasion and disseminated disease associated with Penicillium chrysogenum. Ann Clin Microbiol Antimicrob. 2005;4:21-.
  6. Lyratzopoulos G, Ellis M, Nerringer R, Denning DW. Invasive infection due to penicillium species other than P. marneffei. J Infect. 2002;45(3):184-95.
  7. Ehrmann J, Ritz K. Plant: soil interactions in temperate multi-cropping production systems. Plant Soil. 2014;376(1):1-29.
  8. Park MS, Lee JW, Kim SH, Park J-H, You Y-H, Lim YW. Penicillium from Rhizosphere Soil in Terrestrial and Coastal Environments in South Korea. Mycobiology. 2020;48(6):431-42.
  9. Ferreira ABM, Fischer IH, Leite LG, Padovani CR, Bueno CJJAdIB. Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi. Arq Inst Biol. 2019;86.
  10. Visagie CM, Houbraken J, Frisvad JC, Hong SB, Klaassen CHW, Perrone G, et al. Identification and nomenclature of the genus Penicillium. Stud Mycol. 2014;78:343-71.
  11. Srinivasan R, Prabhu G, Prasad M, Mishra M, Chaudhary M, Srivastava R. Chapter 32 - Penicillium. In: Amaresan N, Senthil Kumar M, Annapurna K, Kumar K, Sankaranarayanan A, editors. Beneficial Microbes in Agro-Ecology: Academic Press; 2020. p. 651-67.
  12. Villarino M, De Cal A, Melgarejo P, Larena I, Espeso EA. The development of genetic and molecular markers to register and commercialize Penicillium rubens (formerly Penicillium oxalicum) strain 212 as a biocontrol agent. Microb Biotechnol. 2016;9(1):89-99.
  13. Wu M, Crismaru CG, Salo O, Bovenberg RA, Driessen AJ. Impact of classical strain improvement of Penicillium rubens on amino acid metabolism during β-lactam production. Appl Environ Microbiol. 2020;86(3):01561-19.
  14. Safaei Z, Karimi K, Zamani A. Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus. Int J Mol Sci. 2016;17(9):1429.
  15. Muggia L, Kopun T, Grube M. Effects of Growth Media on the Diversity of Culturable Fungi from Lichens. Molecules. 2017;22(5):824.
  16. Bhalla K, Qu X, Kretschmer M, Kronstad JW. The phosphate language of fungi. Trends Microbiol. 2021.
  17. Sting R, Eisenberg T, Hrubenja M. Rapid and reasonable molecular identification of bacteria and fungi in microbiological diagnostics using rapid real-time PCR and Sanger sequencing. J Microbiol Methods. 2019;159:148-56.
  18. Wickes BL, Wiederhold NP. Molecular diagnostics in medical mycology. Nat. Commun. 2018;9(1):5135.
  19. Pertsemlidis A, Fondon JW. Having a BLAST with bioinformatics (and avoiding BLASTphemy). Genome Biol. 2001;2(10):reviews2002.1.
  20. Yilmaz N, Visagie CM, Houbraken J, Frisvad JC, Samson RA. Polyphasic taxonomy of the genus Talaromyces. Stud Mycol. 2014;78:175-341.
  21. Ghuffar S, Irshad G, Naz F, Khan MA. Studies of Penicillium species associated with blue mold disease of grapes and management through plant essential oils as non-hazardous botanical fungicides. Green Process Synth. 2021;10(1):021-36.
  22. Yan Y, Kuramae EE, Klinkhamer PGL, van Veen JA. Revisiting the dilution procedure used to manipulate microbial biodiversity in terrestrial systems. Appl Environ Microbiol. 2015;81(13):4246-52.
  23. Bridge P, Spooner BJP, soil. Soil fungi: diversity and detection. Plant Soil. 2001;232(1):147-54.
  24. Pitt JI, Hocking AD. Methods for Isolation, Enumeration and Identification. Fungi and Food Spoilage. Boston, MA: Springer US; 2009. p. 19-52.
  25. Silva DM, Batista LR, Rezende EF, Fungaro MHP,
    Sartori D, Alves E. Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy. Braz J Microbiol. 2011;42:761-73.
  1. Miranda-Hernández F, Angel-Cuapio A, Loera-Corral O. 33 - Production of Fungal Spores for Biological Control. In: Pandey A, Negi S, Soccol CR, editors. Current Developments in Biotechnology and Bioengineering: Elsevier; 2017. p. 757-79.
  2. Pitt J, Hocking AJNSW, Australia. Fungi and food spoilage. Blackie Academic & Professional. 1997.
  3. Lee PY, Costumbrado J, Hsu C-Y, Kim YH. Agarose gel electrophoresis for the separation of DNA fragments. J Vis Exp. 2012(62):3923.
  4. Sawant AM, Vankudoth R, Navale V, Kumavat R, Kumari P, Santhakumari B, et al. Morphological and molecular characterization of Penicillium rubens sp.nov isolated from poultry feed. Indian Phytopathol. 2019;72(3):461-78.
  5. Wang B, Wang L. Penicillium kongii, a new terverticillate species isolated from plant leaves in China. Mycologia. 2013;105(6):1547-54.
  6. Guevara-Suarez M, Sutton DA, Cano-Lira JF, García D, Martin-Vicente A, Wiederhold N, et al. Identification and antifungal susceptibility of Penicillium-like fungi from clinical samples in the United States. J Clin Microbiol. 2016;54(8):2155-61.
  7. Sulaiman IM, Jacobs E, Simpson S. Application of Ribosomal Internal Transcribed Spacer 1, Internal Transcribed Spacer 2, and Large-Subunit D1–D2 Regions as the Genetic Markers to Identify Fungi Isolated from Different Environmental Samples: A Molecular Surveillance Study of Public Health Importance. J. AOAC Int. 2020;103(3):843-50.
  8. Kostadinova N, Tosi S, Spassova B, Angelova MJPPR. Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures. Pol Polar Res. 2017;38(3):393-408-393-408.
  9. Porto BA, da Silva TH, Machado MR, de Oliveira FS, Rosa CA, Rosa LH. Diversity and distribution of cultivable fungi present in acid sulphate soils in chronosequence under para-periglacial conditions in King George Island, Antarctica. Extremophiles. 2020;24(5):797-807.
  10. Arslan UJFEB. Evaluation of antifungal activity of mono and dipotassium phosphates against phytopathogenic fungi. Fresenius Environ Bull. 2015;24(3):810-6.
آمار
تعداد مشاهده مقاله: 1,526
تعداد دریافت فایل اصل مقاله: 398


counter
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب