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Hussein Almola, A, Al-Omari, A. W, Younis Mahdy Al-Hamadany, A. (1400). Molecular Study of Acinetobacter baumannii that Lacking Some Essentials Genes Responsible of Toxin-Antitoxin System. سامانه مدیریت نشریات علمی, 77(1), 483-490. doi: 10.22092/ari.2021.356809.1915
A Hussein Almola; A. W Al-Omari; A Younis Mahdy Al-Hamadany. "Molecular Study of Acinetobacter baumannii that Lacking Some Essentials Genes Responsible of Toxin-Antitoxin System". سامانه مدیریت نشریات علمی, 77, 1, 1400, 483-490. doi: 10.22092/ari.2021.356809.1915
Hussein Almola, A, Al-Omari, A. W, Younis Mahdy Al-Hamadany, A. (1400). 'Molecular Study of Acinetobacter baumannii that Lacking Some Essentials Genes Responsible of Toxin-Antitoxin System', سامانه مدیریت نشریات علمی, 77(1), pp. 483-490. doi: 10.22092/ari.2021.356809.1915
Hussein Almola, A, Al-Omari, A. W, Younis Mahdy Al-Hamadany, A. Molecular Study of Acinetobacter baumannii that Lacking Some Essentials Genes Responsible of Toxin-Antitoxin System. سامانه مدیریت نشریات علمی, 1400; 77(1): 483-490. doi: 10.22092/ari.2021.356809.1915

Molecular Study of Acinetobacter baumannii that Lacking Some Essentials Genes Responsible of Toxin-Antitoxin System

Archives of Razi Institute
مقاله 59، دوره 77، شماره 1، فروردین و اردیبهشت 2022، صفحه 483-490    اصل مقاله (552.03 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356809.1915
نویسندگان
A Hussein Almola* 1؛ A. W Al-Omari1؛ A Younis Mahdy Al-Hamadany2
1College of Sciences, University of Mosul, Mosul, Iraq
2Northern Technical University, Mosul, Iraq
چکیده
Acinetobacter genus has various species that are widespread in different environments and can exist in non-living environment samples as well. Acinetobacter baumannii (A. baumannii) is known to be one of the main causes of nosocomial infection. Few studies have examined the possibility of the presence of this opportunistic pathogen in non-living environment samples. In this study, A. baumannii strain cl-2 was isolated from dishwasher basket samples and it was identified by 16S ribosomal RNA sequencing analysis. The present study also investigated the presence of some important genes responsible for toxin-antitoxin (TA) systems necessary for the resistance of this bacterium in improper environmental conditions. Additionally, attempts were made to study some essential virulence factors, such as hemolysin, lipase, protease, and lecithinase production, as well as biofilm formation and surface motility. The findings revealed that the isolate belongs to the A. baumannii strain cl-2. The isolate was deposed in the National Center for Biotechnology Information, (NCBI) and the data can be accessed via the NCBI accession number (MW642251). The results of screening the TA system by higBA, mazEF, and relBE genes showed the isolate did not contain these genes. The hemolysin toxin activity (phenotypic test) was performed by using the streaking and spot methods on blood agar. It was found that the A. baumannii strain cl-2 had the ability to hemolyze red blood cells and produce lecithinase and protease enzymes. Finally, it was revealed that the A. baumannii strain cl-2 had surface motility based on the concentric diffusion ring of growth observed on Luria Broth agar (0.3%). In conclusion, the isolates under study showed association patterns between their ability to produce hemolysin, lipase, lecithinase, as well as protease, and other virulence factors, including surface motility and biofilm formation.
کلیدواژه‌ها
Acinetobacter baumannii؛ Dishwasher؛ Hemolysin؛ Surface motility Toxin-antitoxin system
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مراجع
  1. Stanton AR. Assessment of motility and hemolytic activity in clinical isolates of Acinetobacter baumannii from University of Kentucky hospital, Lexington, KY. 2013.
  2. Krasauskas R, Skerniškytė J, Martinkus J, Armalytė J, Sužiedėlienė E. Capsule protects Acinetobacter baumannii from inter-bacterial competition mediated by CdiA toxin. Front Microbiol. 2020;11:1493.
  3. Jawad A, Seifert H, Snelling A, Heritage J, Hawkey P. Survival of Acinetobacter baumannii on dry surfaces: comparison of outbreak and sporadic isolates. J Clin Microbiol. 1998;36(7):1938-41.
  4. McConnell MJ, Actis L, Pachón J. Acinetobacter baumannii: human infections, factors contributing to pathogenesis and animal models. FEMS Microbiol Rev. 2013;37(2):130-55.
  5. Eveillard M, Kempf M, Belmonte O, Pailhoriès H, Joly-Guillou M-L. Reservoirs of Acinetobacter baumannii outside the hospital and potential involvement in emerging human community-acquired infections. Int J Infect Dis. 2013;17(10):802-5.
  6. Gerdes K, Christensen SK, Løbner-Olesen A. Prokaryotic toxin–antitoxin stress response loci. Nat Rev Microbiol. 2005;3(5):371-82.
  7. Ghafourian S, Good L, Sekawi Z, Hamat RA, Soheili S, Sadeghifard N, et al. The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii. Mem Inst Oswaldo Cruz. 2014;109:502-5.
  8. Tayabali AF, Nguyen KC, Shwed PS, Crosthwait J, Coleman G, Seligy VL. Comparison of the virulence potential of Acinetobacter strains from clinical and environmental sources. PloS One. 2012;7(5):e37024.
  9. Collee JG, Mackie TJ, McCartney JE. Mackie & McCartney practical medical microbiology: Harcourt Health Sciences; 1996.
  10. Clemmer KM, Bonomo RA, Rather PN. Genetic analysis of surface motility in Acinetobacter baumannii. Microbiology. 2011;157(Pt 9):2534.
  11. Eijkelkamp BA, Hassan KA, Paulsen IT, Brown MH. Investigation of the human pathogen Acinetobacter baumannii under iron limiting conditions. BMC Gen. 2011;12(1):1-14.
  1. Dahdouh E, Hajjar M, Suarez M, Daoud Z. Acinetobacter baumannii isolated from Lebanese patients: phenotypes and genotypes of resistance, Clonality, and determinants of pathogenicity. Front Cell Infect Microbiol. 2016;6:163.
  2. Greene C, Vadlamudi G, Newton D, Foxman B, Xi C. The influence of biofilm formation and multidrug resistance on environmental survival of clinical and environmental isolates of Acinetobacter baumannii. Am J Infect Control. 2016;44(5):e65-e71.
  3. Greene C, Wu J, Rickard AH, Xi C. Evaluation of the ability of Acinetobacter baumannii to form biofilms on six different biomedical relevant surfaces. Lett Appl Microbiol. 2016;63(4):233-9.
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