اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,846
تعداد مقالات32,300
تعداد مشاهده مقاله41,387,125
تعداد دریافت فایل اصل مقاله18,562,183
Hamdan, N. T, Abdalkareem Jasim, S, Khayoon Abed Al-Abboodi, A. (1401). Pharmacognostic Profile and Screening of Anti-Proliferative Potential of Methanolic Extract of Tripterygium wilfordii Plant on WRL-68 Cell Line and Function of Polycystin‐1. سامانه مدیریت نشریات علمی, 77(2), 753-760. doi: 10.22092/ari.2021.356860.1931
N. T Hamdan; S Abdalkareem Jasim; A Khayoon Abed Al-Abboodi. "Pharmacognostic Profile and Screening of Anti-Proliferative Potential of Methanolic Extract of Tripterygium wilfordii Plant on WRL-68 Cell Line and Function of Polycystin‐1". سامانه مدیریت نشریات علمی, 77, 2, 1401, 753-760. doi: 10.22092/ari.2021.356860.1931
Hamdan, N. T, Abdalkareem Jasim, S, Khayoon Abed Al-Abboodi, A. (1401). 'Pharmacognostic Profile and Screening of Anti-Proliferative Potential of Methanolic Extract of Tripterygium wilfordii Plant on WRL-68 Cell Line and Function of Polycystin‐1', سامانه مدیریت نشریات علمی, 77(2), pp. 753-760. doi: 10.22092/ari.2021.356860.1931
Hamdan, N. T, Abdalkareem Jasim, S, Khayoon Abed Al-Abboodi, A. Pharmacognostic Profile and Screening of Anti-Proliferative Potential of Methanolic Extract of Tripterygium wilfordii Plant on WRL-68 Cell Line and Function of Polycystin‐1. سامانه مدیریت نشریات علمی, 1401; 77(2): 753-760. doi: 10.22092/ari.2021.356860.1931

Pharmacognostic Profile and Screening of Anti-Proliferative Potential of Methanolic Extract of Tripterygium wilfordii Plant on WRL-68 Cell Line and Function of Polycystin‐1

Archives of Razi Institute
مقاله 32، دوره 77، شماره 2، خرداد و تیر 2022، صفحه 753-760    اصل مقاله (519.5 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356860.1931
نویسندگان
N. T Hamdan* 1؛ S Abdalkareem Jasim2؛ A Khayoon Abed Al-Abboodi3
1Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq
2Department of Medical Laboratory Techniques, Al-Maarif University College, Anbar, Iraq
3Ministry of Education, Iraq
چکیده
Tripterygium wilfordii is a medicinal plant that plays a crucial role in health care programs, especially in developing countries, and had anti-tumor, anti-inflammatory, anti-fertility, anti-bacterial, and other therapeutic effects. This study was designed to determine the anti-proliferative effects of methanolic extract of T. wilfordii on the WRL-68 cell line and the function of polycystin‐1 (PC-1). The half-maximal inhibitory concentration (IC50) values were recorded in WRL-68 and AsPC-1 cell lines as 193 µg/ml and 149.2 µg/ml, respectively, at 2-2.55 and 2-2.2 µg/ml methanolic plant concentrations. The maximum cytotoxic activities of the extract on the growth inhibition of WRL-68 and AsPC-1 were generally observed at 97.64% and 95.94% at extract concentrations of 50 µg/ml and 25 µg/ml, respectively. The pharmacognostic profile of T. wilfordii extract was found to be alkaloids, tannins, terpenoides, flavonoids, glycosides, and phenols. The extracts of T. wilfordii were tested through gas chromatography-mass spectrometry showing four peaks representing mostly of 3-Oxobutanol; ethyl acetate; acetic acid ethyl ester; chlorbromuron; 1-(methylthio)-, (E)-; n-Hexadecanoic acid; tetradecanoic acid; and 9-Octadecenoic acid. Therefore, the results of this study revealed that the methanolic extract of T. wilfordii was more potential in inducing anti-proliferative activity of WRL-68 and AsPC-1 human cell lines than the control. In addition, the current study was the first study that reported the anti-proliferative potential of T. wilfordii in the treatment of human embryonic liver WRL-68 cancer cells.
کلیدواژه‌ها
Anti-proliferative؛ GC-MS؛ MTT؛ Pharmacognostic profile؛ Tripterygium wilfordii plant
سایر فایل های مرتبط با مقاله
مراجع
  1. VdL-Guideline. 2021. Available from: https://www.Cancer.Org.
  2. Sun CC, Bodurka DC, Weaver CB, Rasu R, Wolf JK, Bevers MW, et al. Rankings and symptom assessments of side effects from chemotherapy: insights from experienced patients with ovarian cancer. Support Care Cancer. 2005;13(4):219-27.
  3. Seca AM, Pinto DC. Plant secondary metabolites as anticancer agents: successes in clinical trials and therapeutic application. Int J Mol Sci. 2018;19(1):263.
  4. Tijjani A, Ya S, Fi A, Iz K, Gm T, Li C. Isolation and Structural Elucidation of 20 Hydroxyecdysterone from Vitex doniana Sweet Stem bark (Black plum) Mustapha. Med Chem. 2017;7(3):828-31.
  5. Yu J, DRISkO J, Chen Q. Inhibition of pancreatic cancer and potentiation of gemcitabine effects by the extract of Pao Pereira. Oncol Rep. 2013;30(1):149-56.
  6. Liu L, Luo Y, Zhou M, Lu Y, Xing M, Ru Y, et al. Tripterygium agents for the treatment of atopic eczema: a Bayesian analysis of randomized controlled trials. Phytomedicine. 2019;59:152914.
  7. Zhang J, Han G, Yu Z. Efficacy and safety of Tripterygium wilfordii Hook F preparations for the treatment of Crohn disease: a systemic review and meta-analysis protocol. Medicine. 2019;98(26).
  8. Li J, Hao J. Treatment of neurodegenerative diseases with bioactive components of Tripterygium wilfordii. Am J Chin Med. 2019;47(04):769-85.
  9. Lin N, Zhang Y-Q, Jiang Q, Liu W, Liu J, Huang Q-C, et al. Clinical Practice Guideline for Tripterygium Glycosides/Tripterygium wilfordii Tablets in the Treatment of Rheumatoid Arthritis. Front Pharmacol. 2021;11:2102.
  10. Luo D, Zuo Z, Zhao H, Tan Y, Xiao C. Immunoregulatory effects of Tripterygium wilfordii Hook F and its extracts in clinical practice. Front Med. 2019;13(5):556-63.
  11. Abid NBS, Rouis Z, Lassoued MA, Sfar S, Aouni M. Assessment of the cytotoxic effect and in vitro evaluation of the anti-enteroviral activities of plants rich in flavonoids. J Appl Pharm Sci. 2012;2(5):74.
  12. Evans WC. Trease and evans' pharmacognosy E-book: Elsevier Health Sciences; 2009.
  13. Harborne A. Phytochemical methods a guide to modern techniques of plant analysis: springer science & business media; 1998.
  14. Zhao X, Liu Z, Ren Z, Wang H, Wang Z, Zhai J, et al. Triptolide inhibits pancreatic cancer cell proliferation and migration via down-regulating PLAU based on network pharmacology of Tripterygium wilfordii Hook F. Eur J Pharmacol. 2020;880:173225.
  15. Ding X, Zhou X, Jiang B, Zhao Q, Zhou G. Triptolide suppresses proliferation, hypoxia-inducible factor-1α and c-Myc expression in pancreatic cancer cells. Mol Med Rep. 2015;12(3):4508-13.
  16. Thani NAA, Keshavarz S, Lwaleed BA, Cooper AJ, Rooprai HK. Cytotoxicity of gemcitabine enhanced by polyphenolics from Aronia melanocarpa in pancreatic cancer cell line AsPC-1. J Clin Pathol. 2014;67(11):949-54.
  17. Akasaka H, Mizushina Y, Yoshida K, Ejima Y, Mukumoto N, Wang T, et al. MGDG extracted from spinach enhances the cytotoxicity of radiation in pancreatic cancer cells. Radiat Oncol. 2016;11(1):1-11.
  18. Rai V, Kumar A, Das V, Ghosh S. Evaluation of chemical constituents and in vitro antimicrobial, antioxidant and cytotoxicity potential of rhizome of Astilbe rivularis (Bodho-okhati), an indigenous medicinal plant from Eastern Himalayan region of India. BMC Complement Altern Med. 2019;19(1):1-10.
  19. Rawa’a A, Hassawi DS, Ibaheem NK. Cytotoxic Activity of Taraxacum officinale Ethanolic Plant Extract against Human Breast Cancer (MCF-7) Cells and Human Hepatic (WRL-68) Cells. Iraqi J Cancer Med Genet. 2018;11(1-2018):16.
  20. Wang X, Matta R, Shen G, Nelin LD, Pei D, Liu Y. Mechanism of triptolide-induced apoptosis: effect on caspase activation and Bid cleavage and essentiality of the hydroxyl group of triptolide. J Mol Med. 2006;84(5):405.
  21. Phillips PA, Dudeja V, McCarroll JA, Borja-Cacho D, Dawra RK, Grizzle WE, et al. Triptolide induces pancreatic cancer cell death via inhibition of heat shock protein 70. Cancer Res. 2007;67(19):9407-16.
  22. Al-Saily HM, Omran P. In Vitro Cytotoxicity of Total Alkaloid Extract from Peganum Harmala L. Seeds. Med Legal Update. 2020;20(1):1148-52.
  23. Baharum Z, Akim AM, Taufiq-Yap YH, Hamid RA, Kasran R. In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao. Molecules. 2014;19(11):18317-31.
  24. Chung WT, Lee SH, Dai Kim J, Sung NS, Hwang B, Lee SY, et al. Effect of the extracts from Glycyrrhiza uralensis Fisch on the growth characteristics of human cell lines: Anti-tumor and immune activation activities. Cytotechnology. 2001;37(1):55-64.
  25. Kuchta K, Xiang Y, Huang S, Tang Y, Peng X, Wang X, et al. Celastrol, an active constituent of the TCM plant Tripterygium wilfordii Hook. f., inhibits prostate cancer bone metastasis. Prostate Cancer Prostatic Dis. 2017;20(2):156-64.
  26. Huang Y, Chen Z, Wang Y, Ba X, Huang Y, Shen P, et al. Triptolide exerts an anti-tumor effect on non‑small cell lung cancer cells by inhibiting activation of the IL‑6/STAT3 axis. Int J Mol Med. 2019;44(1):291-300.
  27. Noel P, Hussein S, Ng S, Antal CE, Lin W, Rodela E, et al. Triptolide targets super-enhancer networks in pancreatic cancer cells and cancer-associated fibroblasts. Oncogenesis. 2020;9(11):1-12.
  28. Feng J, Xu M, Wang J, Zhou S, Liu Y, Liu S, et al. Sequential delivery of nanoformulated α-mangostin and triptolide overcomes permeation obstacles and improves therapeutic effects in pancreatic cancer. Biomaterials. 2020;241:119907.
  29. Zhang B, Meng M, Xiang S, Cao Z, Xu X, Zhao Z, et al. Selective activation of tumor-suppressive MAPKP signaling pathway by triptonide effectively inhibits pancreatic cancer cell tumorigenicity and tumor growth. Biochem Pharmacol. 2019;166:70-81.
  30. Yao Z, Gao W, Takaishi Y, Duan H. Diterpenes from Tripterygium wilfordii and their anti-cancer activities. Chin Tradit Herb Drugs. 1994;(11).
  31. Yang G, Li Y. Antitumor Triterpenoids from Tripterygium wilfordiiHook f. Chem Ind Forest Prod. 2006;26:19-22.
  32. Fang L, Liu M, Cai L. Hederagenin inhibits proliferation and promotes apoptosis of cervical cancer CaSki cells by blocking STAT3 pathway. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2019;35(2):140-5.
  33. Krajnović T, Kaluđerović GN, Wessjohann LA, Mijatović S, Maksimović-Ivanić D. Versatile antitumor potential of isoxanthohumol: Enhancement of paclitaxel activity in vivo. Pharmacol Res. 2016;105:62-73.
  34. Cao Z-q, Wang X-x, Lu L, Xu J-w, Li X-b, Zhang G-r, et al. β-Sitosterol and gemcitabine exhibit synergistic anti-pancreatic Cancer activity by modulating apoptosis and inhibiting epithelial–mesenchymal transition by deactivating Akt/GSK-3β signaling. Front Pharmacol. 2019;9:1525.
  35. Lee J, Kim JH. Kaempferol inhibits pancreatic cancer cell growth and migration through the blockade of EGFR-related pathway in vitro. PloS One. 2016;11(5):0155264.
  36. Adeoye-Isijola MO, Jonathan SG, Coopoosamy RM, Olajuyigbe OO. Molecular characterization, gas chromatography mass spectrometry analysis, phytochemical screening and insecticidal activities of ethanol extract of Lentinus squarrosulus against Aedes aegypti (Linnaeus). Mol Biol Rep. 2021;48(1):41-55.
  1. Raafat K. Phytochemical analysis of Juglans regia oil and kernel exploring their antinociceptive and anti-inflammatory potentials utilizing combined bio-guided GC–FID, GC–MS and HPLC analyses. Rev Bras Farmacogn. 2018;28:358-68.
آمار
تعداد مشاهده مقاله: 1,747
تعداد دریافت فایل اصل مقاله: 449


counter
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب