1گروه علوم دامی، واحد کاشمر، دانشگاه آزاد اسلامی،کاشمر، ایران
2گروه علوم دامی، دانشگاه فردوسی مشهد
3گروه تحقیقات بیوتکنولوژی قارچ های صنعتی، پژوهشکده بیوتکنولوژی صنعتی، مرکز آموزشی، فرهنگی و تحقیقاتی دانشگاهی ، واحد خراسان رضوی
4گروه علوم دامی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران
چکیده
آزمایشی به منظور بررسی اثر عصاره هیدروالکلی گلبرگ زعفران بر شاخص‌های عملکردی، صفات کیفی تخم‌مرغ و رشد سلول‌های سرطانی در مرغ‌های تخمگذار انجام گرفت. در این آزمایش از 200 قطعه مرغ تخمگذار سویه هایلاین W36 در قالب طرح کاملا تصادفی با چهار تیمار، پنج تکرار و 10 قطعه مرغ در هر تکرار استفاده شد. تیمارهای آزمایشی شامل: تیماریک: شاهد (جیره پایه)، تیماردو: جیره پایه + ppm 40 عصاره گلبرگ زعفران، تیمارسه: جیره پایه + ppm 60 عصاره گلبرگ زعفران، تیمار چهار: جیره پایه + ppm 80 عصاره گلبرگ زعفران بود. نتایج این تحقیق نشان داد که مصرف خوراک، ضریب تبدیل، وزن تخم‌مرغ و توده تخم‌مرغ در کل دوره‌ی آزمایشی تحت تاثیر تیمارها قرار نگرفت (05/0<P). درصد تولید تخم‌مرغ تحت تاثیر تیمارهای آزمایشی قرار گرفت(05/0>P). وزن زرده، وزن پوسته، واحد هاو، ضخامت پوسته و رنگ زرده بطور معنی‌داری تحت تاثیر تیمارهای آزمایشی قرار گرفتند (05/0>P). در تیمار سه بالاترین میزان ارتفاع زرده، وزن زرده، وزن پوسته و ضخامت پوسته مشاهده شد. همچنین بالاترین میزان واحد هاو در تیمارهای یک و چهار و بیشترین رنگ زرده در تیمارهای سه و چهار مشاهده شد.تیمارهای آزمایشی بر بازدارندگی رشد سلول‌های سرطانی MCF-7 و PC3 در غلظت‌های مختلف در شکل وابسته به غلظت تاثیر معنی‌داری داشتند (05/0>P). نتایج به دست آمده از این آزمایش نشان داد که افزودن عصاره گلبرگ زعفران باعث بهبود کیفیت تخم‌مرغ و اثر بازدارندگی بر روی سلول‌های سرطانی می‌شود و بهترین دز‌های مورد استفاده از گلبرگ زعفران در تیمارهای سه و چهار مشاهده گردید.
The effect of hydroalcoholic extract of saffron petals on egg laying performance of Leghorn chickens and the inhibitory effects of obtained eggs on the growth of MCF-7 and PC3 cancer cells
1Department of animal science, Islamic Azad University, Kashmar, Iran
2Department of Animal Science, Ferdowsi University of Mashhad
3Industrial Fungi Biotechnology Research Department, Research Institute for Industrial Biotechnology, Academic Center for Education, Culture and Research (ACECR)-Khorasan Razavi Branch
4Animal Science Department, Mashhad Branch, Islamic Azad University, Mashhad, Iran
چکیده [English]
An experiment was performed to investigate the effect of hydroalcoholic extract of saffron petals on performance indices, egg quality and cancer cells in laying hens. , Five replicates and 10 chicken pieces per replicate were used. Experimental treatments included: treatment: control (basic diet), Timardo: basic diet + 40 ppm saffron petal extract, treatment: basic diet + 60 ppm saffron petal extract, treatment four: basic diet + 80 ppm saffron petal extract. The results of this study showed that feed consumption, conversion ratio, egg weight and egg mass were not affected by the treatments during the whole experimental period (P <0.05). The percentage of egg production was affected by experimental treatments and increased significantly (P <0.05).Yolk weight, shell weight, units, shell thickness and yolk color were significantly affected by experimental treatments (P <0.05). As in the three treatments, the highest yolk height, yolk weight, shell weight and shell thickness were observed. Also, the highest amount of units was observed in treatments one and four and the highest yolk color was observed in treatments three and four. Experimental treatments had a significant effect on growth inhibition of MCF-7 and PC3 cancer cells at different concentrations in concentration-dependent form (P <0.05). The results of this experiment showed that the addition of saffron petal extract improves egg quality and inhibitory effect on cancer cells and the best doses of saffron petal used were observed in treatments three and four.
کلیدواژه‌ها [English]
Hydroalcoholic extract of saffron, Qualitative traits of eggs, Cancer cells, Layers
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