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Saleem Abd Alkreem Alsaqi, S. (1400). Immunohistochemical and Electron Microscopy Evolution in the Diagnosis of Lung Cancer in Iraq. سامانه مدیریت نشریات علمی, 77(1), 95-100. doi: 10.22092/ari.2021.356552.1870
S Saleem Abd Alkreem Alsaqi. "Immunohistochemical and Electron Microscopy Evolution in the Diagnosis of Lung Cancer in Iraq". سامانه مدیریت نشریات علمی, 77, 1, 1400, 95-100. doi: 10.22092/ari.2021.356552.1870
Saleem Abd Alkreem Alsaqi, S. (1400). 'Immunohistochemical and Electron Microscopy Evolution in the Diagnosis of Lung Cancer in Iraq', سامانه مدیریت نشریات علمی, 77(1), pp. 95-100. doi: 10.22092/ari.2021.356552.1870
Saleem Abd Alkreem Alsaqi, S. Immunohistochemical and Electron Microscopy Evolution in the Diagnosis of Lung Cancer in Iraq. سامانه مدیریت نشریات علمی, 1400; 77(1): 95-100. doi: 10.22092/ari.2021.356552.1870

Immunohistochemical and Electron Microscopy Evolution in the Diagnosis of Lung Cancer in Iraq

Archives of Razi Institute
مقاله 14، دوره 77، شماره 1، فروردین و اردیبهشت 2022، صفحه 95-100    اصل مقاله (824.39 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356552.1870
نویسنده
S Saleem Abd Alkreem Alsaqi*
Department of Anatomy, College of Medicine, University of Babylon, Babylon, Iraq
چکیده
Lung cancer is considered the most commonly diagnosed cancer that kills over 1.76 million people yearly around the world. This study aimed to investigate how electron microscopy (EM) and immunohistochemistry (IHC) worked together. In the current study, a total of 50 tissue blocks were used which were embedded in paraffin wax from 20 healthy controls and 30 lung cancer patients (19 males and 11 females) to conduct a histopathological examination, which included hematoxylin and eosin stain, IHC detection regarding the protein expression in P53, and EM for lung cancer diagnosis. The findings indicated that the mean age of the patients was 51.84±15.70 years, and there was no correlation between the age of patients and the disease stage. P53 expression was measured using an immunohistochemical technique, while the positive rate showed a highly significant difference between healthy controls and patients (P≤0.05). The association of P53 expression with age and gender was not significant, although it showed a significant correlation with stage and grade. Furthermore, a correlation was found between P53 IHC and EM (P≤0.05). In conclusion, the detection of an immunohistochemical method of lung cancer patients has been (gold standard) useful in detecting the subsets of patients in addition to a good method of EM in the diagnosis of lung cancer patients.
کلیدواژه‌ها
Electron microscopy؛ Immunohistochemical؛ Lung adenocarcinoma؛ Prognostic factors
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مراجع
  1. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. Cancer J Clin. 2016;66(1):7-30.
  2. Le Péchoux C. Role of postoperative radiotherapy in resected non-small cell lung cancer: a reassessment based on new data. Oncologist. 2011;16(5):672.
  3. Shamseddine A, Saleh A, Charafeddine M, Seoud M, Mukherji D, Temraz S, et al. Cancer trends in Lebanon: a review of incidence rates for the period of 2003–2008 and projections until 2018. Popul Health Metr. 2014;12(1):1-8.
  4. Miller KD, Goding Sauer A, Ortiz AP, Fedewa SA, Pinheiro PS, Tortolero‐Luna G, et al. Cancer statistics for hispanics/latinos. Cancer J Clin. 2018;68(6):425-45.
  5. Torre LA, Siegel RL, Jemal A. Lung Cancer Statistics. In: Ahmad A, Gadgeel S, editors. Lung Cancer and Personalized Medicine: Current Knowledge and Therapies. Cham: Springer International Publishing; 2016. p. 1-19.
  6. Thunnissen E, Allen TC, Adam J, Aisner DL, Beasley MB, Borczuk AC, et al. Immunohistochemistry of pulmonary biomarkers: a perspective from members of the Pulmonary Pathology Society. Arch Path Lab. 2018;142(3):408-19.
  7. Mino-Kenudson M. Immunohistochemistry for predictive biomarkers in non-small cell lung cancer. Transl Lung Cancer Res. 2017;6(5):570.
  8. Hammar SP, Bockus DE, Remington FL, Rohrbach KA. Mucin-positive epithelial mesotheliomas: a histochemical, immunohistochemical, and ultrastructural comparison with mucin-producing pulmonary adenocarcinomas. Ultrastruct Pathol. 1996;20(4):293-325.
  9. Henderson D. Transmission electron microscopy of malignant mesothelioma. MSA Bull. 1993;23:288-97.
  10. Falkeholm L, Grant CA, Magnusson A, Möller E. Xylene-free method for histological preparation: a multicentre evaluation. Lab Invest. 2001;81(9):1213-21.
  11. Ausubel F, Brent R, Kingston R, Moore D, Seidman J, Smith J, et al. Current Protocols in Molecular Biology. New York: John Willey & Sons. Inc; 1994.
  12. Mousawi AA. The prevalence of smoking among Karbala/Iraq university students in Iraq in 2005. Tob Use Insights. 2014;7:12238.
  1. Babacan NA, Yucel B, Kilickap S, Seker MM, Kacan T, Olcas IK, et al. Lung cancer in women: a single institution experience with 50 patients. Asian Pac J Cancer Prev. 2014;15(1):151-4.
  2. Chen K-Y, Hsiao C-F, Chang G-C, Tsai Y-H, Su W-C, Chen Y-M, et al. Estrogen receptor gene polymorphisms and lung adenocarcinoma risk in never-smoking women. J Thorac Oncol. 2015;10(10):1413-20.
  3. Habib OS, Al-Asadi JN, Hussien OG. Lung cancer in Basrah, Iraq during 2005-2012: Incidence and time trend. Saudi Med J. 2016;37(11):1214.
  4. Lee SH, Kim H, Kim WY, Han HS, Lim SD, Kim WS, et al. Genetic alteration and immunohistochemical staining patterns of ovarian high‐grade serous adenocarcinoma with special emphasis on p53 immnnostaining pattern. Pathol Int. 2013;63(5):252-9.
  5. Dubinski W, Leighl NB, Tsao M-S, Hwang DM. Ancillary testing in lung cancer diagnosis. Pulm Med. 2012;2012.
  6. Gibbons DL, Byers LA, Kurie JM. Smoking, p53 mutation, and lung cancer. Mol Cancer Res. 2014;12(1):3-13.
  7. Clavé S, Gimeno J, Muñoz-Mármol AM, Vidal J, Reguart N, Carcereny E, et al. ROS1 copy number alterations are frequent in non-small cell lung cancer. Oncotarget. 2016;7(7):8019.
  8. Righi L, Graziano P, Fornari A, Rossi G, Barbareschi M, Cavazza A, et al. Immunohistochemical subtyping of nonsmall cell lung cancer not otherwise specified in fine‐needle aspiration cytology: a retrospective study of 103 cases with surgical correlation. Cancer. 2011;117(15):3416-23.
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