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,Bushra. A, Kadhim, ,M.A, Essa, ,M. R, Sabah. (1400). Study the inhibition of sea gene expressionin staphylococcus aureus isolated from different sources by using aqueous and alcoholic extracts of seeds in grape type vitis vinifera l. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2021.356364.1830
Kadhim ,Bushra. A; Essa ,M.A; Sabah ,M. R. "Study the inhibition of sea gene expressionin staphylococcus aureus isolated from different sources by using aqueous and alcoholic extracts of seeds in grape type vitis vinifera l". سامانه مدیریت نشریات علمی, , , 1400, -. doi: 10.22092/ari.2021.356364.1830
,Bushra. A, Kadhim, ,M.A, Essa, ,M. R, Sabah. (1400). 'Study the inhibition of sea gene expressionin staphylococcus aureus isolated from different sources by using aqueous and alcoholic extracts of seeds in grape type vitis vinifera l', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2021.356364.1830
,Bushra. A, Kadhim, ,M.A, Essa, ,M. R, Sabah. Study the inhibition of sea gene expressionin staphylococcus aureus isolated from different sources by using aqueous and alcoholic extracts of seeds in grape type vitis vinifera l. سامانه مدیریت نشریات علمی, 1400; (): -. doi: 10.22092/ari.2021.356364.1830

Study the inhibition of sea gene expressionin staphylococcus aureus isolated from different sources by using aqueous and alcoholic extracts of seeds in grape type vitis vinifera l

Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 07 آبان 1400  XML
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356364.1830
نویسندگان
Kadhim ,Bushra. A email 1؛ Essa ,M.A2؛ Sabah ,M. R3
11 University of Baghdad, College of Nursing, Baghdad, Iraq
2University of Mosul, College of Science, Mouul ,Iraq
3Al-Nahrein University, DNA center, Baghdad, Iraq
چکیده
This research was conducted with the aim of evaluating the activity of aqueous and alcoholic extracts of grape seeds in inhibiting the expression of the sea gene encoding staphylococcal enterotoxin type A in Staphylococcus aureus isolated from different sources. The results showed that MIC (Minimum Inhibition Concentration) and SMIC (Sub Minimum Inhibition Concentration) of the aqueous extract were 32 and 16 µg / ml respectively for all isolates. While in the case of the alcoholic extract this concentrations were 16 and 8 µg / ml respectively for all isolates as well, and the results of the chemical analysis of the aqueous and alcoholic extracts confirmed that they contain active chemical compounds such as flavonoids, alkaloids, tannins and glycosides, as well as they contain many functional groups according to the analysis of the infrared spectrum. Both extracts were shown to be active in inhibiting gene expression of the sea gene in the isolates under study, as the results indicated that the gene expression of these isolates was inhibited by approximately0.31to 0.63fold, and that all pathogenic and environmental isolates showed a decrease in the expression of this gene. These results practically open the door to the possibility of using these extracts to inhibit the ability of Staphylococcus aureus to produce these dangerous enterotoxins, thus decreasing or preventing their pathogenicity, especially their food poisoning infections.
کلیدواژه‌ها
Grape seeds؛ Staphylococcus aureus؛ Sea gene؛ Food poisoning؛ Expression
آمار
تعداد مشاهده مقاله: 22


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