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Aldoghachi, F. E. H, Noor Al-Mousawi, U. M, Shari, F. H. (1400). Antioxidant Activity of Rosmarinic Acid Extracted and Purified from Mentha piperita. سامانه مدیریت نشریات علمی, 76(5), 1279-1287. doi: 10.22092/ari.2021.356072.1770
F. E. H Aldoghachi; U. M Noor Al-Mousawi; F. H Shari. "Antioxidant Activity of Rosmarinic Acid Extracted and Purified from Mentha piperita". سامانه مدیریت نشریات علمی, 76, 5, 1400, 1279-1287. doi: 10.22092/ari.2021.356072.1770
Aldoghachi, F. E. H, Noor Al-Mousawi, U. M, Shari, F. H. (1400). 'Antioxidant Activity of Rosmarinic Acid Extracted and Purified from Mentha piperita', سامانه مدیریت نشریات علمی, 76(5), pp. 1279-1287. doi: 10.22092/ari.2021.356072.1770
Aldoghachi, F. E. H, Noor Al-Mousawi, U. M, Shari, F. H. Antioxidant Activity of Rosmarinic Acid Extracted and Purified from Mentha piperita. سامانه مدیریت نشریات علمی, 1400; 76(5): 1279-1287. doi: 10.22092/ari.2021.356072.1770

Antioxidant Activity of Rosmarinic Acid Extracted and Purified from Mentha piperita

Archives of Razi Institute
مقاله 16، دوره 76، شماره 5، بهمن و اسفند 2021، صفحه 1279-1287    اصل مقاله (802.16 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356072.1770
نویسندگان
F. E. H Aldoghachi* ؛ U. M Noor Al-Mousawi؛ F. H Shari
College of Pharmacy, Basra University, Basra, Iraq
چکیده
Rosmarinic acid was obtained from methanolic extract of Mentha piperita L. under a reflux condenser. The current study aimed to evaluate the in vitro antioxidant activities and rosmarinic acid levels of the methanol extracts of M. piperita. The analysis of the sample by high-performance liquid chromatography technique (HPLC) indicated that rosmarinic acid was present in high concentration 1.9 mg/mL in the extract. Purification was carried out by column chromatography to give 0.020 g from 1 g of crude extract, and then the antioxidant activity of purified rosmarinic acid was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), H2O2 scavenging, and REDOX methods. It was revealed that the anti-oxidant potential of the rosmarinic acid extract was greater than 95% (at 100 µg/mL) for DPPH assay and 87.83% (at 100 µg/ml) for H2O2 scavenging assay. This study was performed by using a reflux methanolic extraction of M. piperita. This possible instructional technique proved to be a quick and successful method for retaining the antioxidant properties of rosmarinic acid. The rosmarinic acid content was determined using HPLC.
کلیدواژه‌ها
Anti-oxidant؛ DPPH؛ H2O2؛ M. piperita؛ RA؛ Reflux
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