اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,829
تعداد مقالات32,098
تعداد مشاهده مقاله40,864,102
تعداد دریافت فایل اصل مقاله18,113,732
Hasan Ali, I, Sahib Abdulamir, A. (1400). Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq. سامانه مدیریت نشریات علمی, 76(5), 1255-1267. doi: 10.22092/ari.2021.356135.1785
I Hasan Ali; A Sahib Abdulamir. "Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq". سامانه مدیریت نشریات علمی, 76, 5, 1400, 1255-1267. doi: 10.22092/ari.2021.356135.1785
Hasan Ali, I, Sahib Abdulamir, A. (1400). 'Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq', سامانه مدیریت نشریات علمی, 76(5), pp. 1255-1267. doi: 10.22092/ari.2021.356135.1785
Hasan Ali, I, Sahib Abdulamir, A. Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq. سامانه مدیریت نشریات علمی, 1400; 76(5): 1255-1267. doi: 10.22092/ari.2021.356135.1785

Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq

Archives of Razi Institute
مقاله 14، دوره 76، شماره 5، بهمن و اسفند 2021، صفحه 1255-1267    اصل مقاله (1.17 M)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356135.1785
نویسندگان
I Hasan Ali* ؛ A Sahib Abdulamir
Department of Microbiology, Al-Nahrain University College of Medicine, Baghdad, Iraq
چکیده
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the Coronaviridae family which led to a global pandemic. However, available knowledge on adaptive immunity in convalescent individuals is limited. The present study was conducted on 191 convalescent coronavirus disease 2019 (COVID-19) health care workers (HCW); moreover, it evaluated the cell-mediated immunity of 122 recovered HCW and the level of anti-receptor binding domain (RBD) IgG antibodies of 181 recovered HCW. Cellular and humoral immune responses were identified over time from one to eight months post recovery with varying disease severity using MTT proliferation assay and enzyme-linked immunosorbent assay. Analysis of lymphocyte proliferation with S1 protein in mild-moderate and severe HCW revealed an insignificant difference with an increase in the maximum and third quartile (Q3) from one to eight months after COVID-19 recovery. Antibody levels in mild-moderate and severe recovered HCW were insignificantly different from post-COVID 19 recovery (P>0.05); in addition, the median, maximum, and Q3 values of anti-RBD IgG were close to each other over the time intervals from one to eight months post recovery. These data suggest that many convalescent HCW enrolled in this study were re-exposed to the virus without the development of symptoms indicating the role of cell-mediated and humoral immunity in preventing symptomatic reinfection. This study reveals that a robust immunity developed after mild, moderate, and severe COVID-19 that could last for several months post recovery.
کلیدواژه‌ها
Cell-Mediated Immunity؛ Coronavirus Disease 2019 (COVID-19)؛ Humoral Immunity؛ Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
سایر فایل های مرتبط با مقاله
مراجع
  1. Phelan AL, Katz R, Gostin LO. The Novel Coronavirus Originating in Wuhan, China: Challenges for Global Health Governance. J Am Med Assoc. 2020;323(8):709–10.
  2. Chan JFW, Yuan S, Kok KH, To KKW, Chu H, Yang J, et al. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. Lancet. 2020;395(10223):514–23.
  3. Wang Q, Zhang Y, Wu L, Niu S, Song C, Zhang Z, et al. Structural and functional basis of SARS-CoV-2 entry by using human ACE2. Cell. 2020;181(4):894–904.
  4. Alejandra Tortorici M, Walls AC, Lang Y, Wang C, Li Z, Koerhuis D, et al. Structural basis for human coronavirus attachment to sialic acid receptors. Nat Struct Mol Biol. 2019;26(6):481–9.
  5. Letko M, Marzi A, Munster V. Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses. Nat Microbiol. 2020;5(4):562–9.
  6. Walls AC, Park YJ, Tortorici MA, Wall A, McGuire AT, Veesler D. Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein. Cell. 2020;181(2):281-292.
  7. Hoffmann M, Kleine-Weber H, Schroeder S, Krüger N, Herrler T, Erichsen S, et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. 2020;181(2):271-80.
  8. Cevik M, Bamford CGG, Ho A. COVID-19 pandemic—a focused review for clinicians. Clin Microbiol Infect. 2020;26(7):842–7.
  9. Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020;395(10223):497–506.
  10. Liao M, Liu Y, Yuan J, Wen Y, Xu G, Zhao J, et al. Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19. Nat Med. 2020;26(6):842–4.
  11. Tay MZ, Poh CM, Rénia L, MacAry PA, Ng LFP. The trinity of COVID-19: immunity, inflammation and intervention. Nat Rev Immunol. 2020;20(6):363–74.
  12. Vabret N, Britton GJ, Gruber C, Hegde S, Kim J, Kuksin M, et al. Immunology of COVID-19: Current State of the Science. Immunity. 2020;52(6):910–41.
  13. Valentine KM, Hoyer KK. CXCR5+ CD8 T cells: protective or pathogenic? Front Immunol. 2019;10:1322.
  1. Lucas C, Wong P, Klein J, Castro TBR, Silva J, Sundaram M, et al. Longitudinal analyses reveal immunological misfiring in severe COVID-19. Nature. 2020;584(7821):463–9.
  2. Liu T, Zhang J, Yang Y, Ma H, Li Z, Zhang J, et al. The role of interleukin‐6 in monitoring severe case of coronavirus disease 2019. EMBO Mol Med. 2020;12(7):e12421.
  3. Tan L, Wang Q, Zhang D, Ding J, Huang Q, Tang Y-Q, et al. Lymphopenia predicts disease severity of COVID-19: a descriptive and predictive study. Signal Transduct Target Ther. 2020;5(1):1–3.
  4. Richardson S, Hirsch JS, Narasimhan M, Crawford JM, McGinn T, Davidson KW, et al. Presenting Characteristics, Comorbidities, and Outcomes among 5700 Patients Hospitalized with COVID-19 in the New York City Area. J Am Med Assoc. 2020;323(20):2052–9.
  5. Rodda LB, Netland J, Shehata L, Pruner KB, Morawski PA, Thouvenel CD, et al. Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19. Cell. 2021;184(1):169-83.
  6. Dan JM, Mateus J, Kato Y, Hastie KM, Yu ED, Faliti CE, et al. Immunological memory to SARS-CoV-2 assessed for up to eight months after infection. BioRxiv. 2020.
  7. Long Q-X, Tang X-J, Shi Q-L, Li Q, Deng H-J, Yuan J, et al. Clinical and immunological assessment of asymptomatic SARS-CoV-2 infections. Nat Med. 2020;26(8):1200–4.
  8. Sewell HF, Agius RM, Stewart M, Kendrick D. Cellular immune responses to covid-19. Br Med J; 2020.
  9. Lombardi A, Consonni D, Carugno M, Bozzi G, Mangioni D, Muscatello A, et al. Characteristics of 1573 healthcare workers who underwent nasopharyngeal swab testing for SARS-CoV-2 in Milan, Lombardy, Italy. Clin Microbiol Infect. 2020;26(10):1413-e9.
  10. WHO. Clinical management Clinical management : Living guidance COVID-19 2021 .Available from: https://apps.who.int/iris/handle/10665/338882.
  11. Xie S, Huang J, Qiao Q, Zang W, Hong S, Tan H, et al. Expression of the inhibitory B7 family molecule VISTA in human colorectal carcinoma tumors. Cancer Immunol Immunother. 2018;67(11):1685–94.
  12. Zhan CS, Chen J, Chen J, Zhang LG, Liu Y, Du HX, et al. CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis. FASEB J. 2020;34(10):14006–23.
  13. Ni L, Ye F, Cheng ML, Feng Y, Deng YQ, Zhao H, et al. Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals. Immunity. 2020;52(6):971-7.
  14. Braun J, Loyal L, Frentsch M, Wendisch D, Georg P, Kurth F, et al. Presence of SARS-CoV-2 reactive T cells in COVID-19 patients and healthy donors. MedRxiv. 2020;
  15. Sekine T, Perez-Potti A, Rivera-Ballesteros O, Strålin K, Gorin JB, Olsson A, et al. Robust T Cell Immunity in Convalescent Individuals with Asymptomatic or Mild COVID-19. Cell. 2020;183(1):158-68.
  16. Le Bert N, Tan AT, Kunasegaran K, Tham CYL, Hafezi M, Chia A, et al. SARS-CoV-2-specific T cell immunity in cases of COVID-19 and SARS, and uninfected controls. Nature. 2020;584(7821):457–62.
  17. Braun J, Loyal L, Frentsch M, Wendisch D, Georg P, Kurth F, et al. SARS-CoV-2-reactive T cells in healthy donors and patients with COVID-19. Nature. 2020;587(7833):270–4.
  18. Weiskopf D, Schmitz KS, Raadsen MP, Grifoni A, Okba NMA, Endeman H, et al. Phenotype and kinetics of SARS-CoV-2-specific T cells in COVID-19 patients with acute respiratory distress syndrome. Sci Immunol. 2020;5(48).
  19. Bohn MK, Hall A, Sepiashvili L, Jung B, Steele S, Adeli K. Pathophysiology of COVID-19: mechanisms underlying disease severity and progression. Physiology. 2020;35(5):288–301.
  1. Benner SE, Patel EU, Laeyendecker O, Pekosz A, Littlefield K, Eby Y, et al. SARS-CoV-2 antibody avidity responses in COVID-19 patients and convalescent plasma donors. J Infect Dis. 2020;222(12):1974–84.
  2. Anand SP, Prévost J, Nayrac M, Beaudoin-Bussières G, Benlarbi M, Gasser R, et al. Longitudinal analysis of humoral immunity against SARS-CoV-2 Spike in convalescent individuals up to 8 months post-symptom onset. Cell Reports Med. 2021;100290.
  3. Whitcombe AL, McGregor R, Craigie A, James A, Charlewood R, Lorenz N, et al. Comprehensive analysis of SARS‐CoV‐2 antibody dynamics in New Zealand. Clin Transl Immunol. 2021;10(3):1261.
  4. Wajnberg A, Amanat F, Firpo A, Altman DR, Bailey MJ, Mansour M, et al. Robust neutralizing antibodies to SARS-CoV-2 infection persist for months. Science (80- ). 2020;370(6521):1227–30.
  5. Guthmiller JJ, Stovicek O, Wang J, Changrob S, Li L, Halfmann P, et al. SARS-CoV-2 infection severity is linked to superior humoral immunity against the spike. MBio. 2021;12(1):e02940-20.
38.          Li K, Huang B, Wu M, Zhong A, Li L, Cai Y, et al. Dynamic changes in anti-SARS-CoV-2 antibodies during SARS-CoV-2 infection and recovery from COVID-19. Nat Commun. 2020;11(1):1–11.

آمار
تعداد مشاهده مقاله: 1,306
تعداد دریافت فایل اصل مقاله: 337


counter
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب