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Comparative effect of different vaccines on immune-related gene expressions of rainbow trout (Oncorhynchus mykiss) and experimentally infected with Streptococcus iniae and Lactococus garvie | ||
| Iranian Journal of Fisheries Sciences | ||
| دوره 20، شماره 5، آذر 2021، صفحه 1387-1408 اصل مقاله (885.86 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/ijfs.2021.350534.0 | ||
| نویسندگان | ||
| Hossein Khaj* 1؛ Mehrzad Mesbah؛ Mohammad Reza Tabandeh2؛ Takavar Mohammadian؛ Maryam Dadar | ||
| 1Boushehr Agricultural and Natural Resources Research and Education Center, AREEO, Boushehr, Iran | ||
| 2u | ||
| چکیده | ||
| To understand how different vaccines, i.e., bivalent formalin-inactivated vaccine (Lactococus garvie and Streptococcus iniae), could affect the immune-related gene expressions, the TNF-alpha, IL-1β and IL-8 genes of head kidney was measured in Oncorhynchus mykiss. To address this, 630 juvenile fish weighing 26±3 g were initially (day 0) injected by three different treatments, including control group (normal saline only), AquaVacTM Garvetil (Merck) and Iranian vaccine (Jehad-e-Daneshagahi) and followed by bath immersion into the same vaccine on day 30. The head kidney was withdrawn to measure immune-related gene expressions at the beginning of experiment (day 0), and following 14, 30, 45 and 60 days of post-vaccination. In the next step, control and both vaccinated groups were also subjected to either L. garvie or S. iniae challenge. Similar samples were taken immediately following bacteria injection (time= 0) and 12, 48, 72 h, and following 7 and 10 days of challenged test. The real-time PCR indicated up-regulation of all cytokine genes following vaccination at day 14 as compared to initial day (p<0.05). In pre-challenged experiment, the higher induction in the level of those genes in the head kidney was related to AquaVac vaccine (p<0.05). Injection by S. iniae and L. garviae induced the level of immune-related gene expression in the head kidney within the first few days with higher intensity in case of unvaccinated control group. Although the AquaVac produces higher up-regulation of inflammatory gene expression rather than Iranian vaccine, the immunostimulatory effects of both vaccines is a time-restricted. | ||
| کلیدواژه‌ها | ||
| Cytokine genes؛ Pro-inflammatory genes؛ Bacterial infection؛ Passive vaccination؛ Oncorhynchus mykiss | ||
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آمار تعداد مشاهده مقاله: 262 تعداد دریافت فایل اصل مقاله: 130 |
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