اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,848
تعداد مقالات32,322
تعداد مشاهده مقاله41,447,353
تعداد دریافت فایل اصل مقاله18,648,134
Mijbel Ali, B, Gatea Kaabi, S. A, Al-Bayati, M. A, Musafer, H. K. (1400). A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model. سامانه مدیریت نشریات علمی, 76(5), 1229-1236. doi: 10.22092/ari.2021.356004.1762
B Mijbel Ali; S. A Gatea Kaabi; M. A Al-Bayati; H. K Musafer. "A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model". سامانه مدیریت نشریات علمی, 76, 5, 1400, 1229-1236. doi: 10.22092/ari.2021.356004.1762
Mijbel Ali, B, Gatea Kaabi, S. A, Al-Bayati, M. A, Musafer, H. K. (1400). 'A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model', سامانه مدیریت نشریات علمی, 76(5), pp. 1229-1236. doi: 10.22092/ari.2021.356004.1762
Mijbel Ali, B, Gatea Kaabi, S. A, Al-Bayati, M. A, Musafer, H. K. A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model. سامانه مدیریت نشریات علمی, 1400; 76(5): 1229-1236. doi: 10.22092/ari.2021.356004.1762

A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model

Archives of Razi Institute
مقاله 11، دوره 76، شماره 5، بهمن و اسفند 2021، صفحه 1229-1236    اصل مقاله (573.49 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356004.1762
نویسندگان
B Mijbel Ali؛ S. A Gatea Kaabi؛ M. A Al-Bayati؛ H. K Musafer*
Department of Biology, College of Sciences, Mustansiriyah University, Baghdad, Iraq
چکیده
Escherichia coli (E. coli) is a major bacterial pathogen associated with many cases of serious infections, such as urinary tract infections (UTI) and meningitis intestinal. The rapid emergence of antimicrobial multidrug-resistant bacteria occurring worldwide has been attributed to the overuse of antibiotics. Alternative strategies must be developed to overcome antibiotic resistance. A promising alternative for the treatment of infections is the use of phages as antibacterial agents. A total of 90 female albino mice were randomly divided into three groups (n=30) and used for the induction of UTI. The animals were acclimatized in their cages for 24 h before inoculation and allowed to access chow and water freely. For UTI induction, the peri-urethral area was sterilized with 70% ethanol, and bacterial inoculation was then injected into the bladder through the urethra using a 24-gauge sterile Teflon catheter with an outer diameter of 0.7 mm and length of 19 mm. A single phage and a phage cocktail preparation have been evaluated for their therapeutic activity in the mouse model of chronic UTI induced by transurethral injection of two isolates of the uropathogenic E. coli 8 and E. coli 302. The results of the transurethral and intra-peritoneal injection of phage(s) that prepared on day 10 after the establishment of the mouse chronic model showed no effect of a single phage PEC80 in the treatment of UTI, whereas both administration routes of the phage cocktail preparation resulted in the clearance of bacteria from mice urine and homogenates of the urinary bladders and kidneys of the sacrificed mice after 24 h following the administration of phage cocktail dose. The high activity of the phage cocktail in the treatment of mouse chronic model of UTI is attributed to the broader host range of the phage cocktail, compared to the very narrow host range of the phage PEC80. It is concluded that the phage therapy by using phage preparations as the 25 phages cocktail evaluated in this study is a highly promising and potential alternative therapy for human UTIs.
کلیدواژه‌ها
Phage Therapy؛ UTI؛ Drug Resistance؛ Phage Cocktail؛ Alternative Therapy
سایر فایل های مرتبط با مقاله
مراجع
  1. Ujmajuridze A, Chanishvili N, Goderdzishvili M, Leitner L, Mehnert U, Chkhotua A, et al. Adapted Bacteriophages for Treating Urinary Tract Infections. Front Microbiol. 2018;9:1832.
  2. Chanishvili N. A literature review of the practical application of bacteriophage research. Lit Rev Pract App Bacteriophage Res. 2012:1-292.
  3. Sybesma W, Zbinden R, Chanishvili N, Kutateladze M, Chkhotua A, Ujmajuridze A, et al. Bacteriophages as Potential Treatment for Urinary Tract Infections. Front Microbiol. 2016;7:465.
  4. Kaabi S, Ali B. Development of phage cocktail against extensive drug and pandrug resistant Escherichia coli causing urinary tract infection: an invitro study. Cell Arch. 2020;20:3709-14.
  5. Mobley HL, Green DM, Trifillis AL, Johnson DE, Chippendale GR, Lockatell CV, et al. Pyelonephritogenic Escherichia coli and killing of cultured human renal proximal tubular epithelial cells: role of hemolysin in some strains. Infect Immun. 1990;58(5):1281-9.
  6. Bayer ME. Visualization of the bacterial polysaccharide capsule. Curr Top Microbiol Immunol. 1990;150:129-57.
  7. Sharma S, Harjai K, Mittal R. Enhanced siderophore production and mouse kidney pathogenicity in Escherichia coli grown in urine. J Med Microbiol. 1991;35(6):325-9.
  8. Chin JL, Kadhim SA, Batislam E, Karlik SJ, Garcia BM, Nickel JC, et al. Mycobacterium cell wall: an alternative to intravesical bacillus Calmette Guerin (BCG) therapy in orthotopic murine bladder cancer. J Urol. 1996;156(3):1189-93.
  9. Asahara T, Nomoto K, Watanuki M, Yokokura T. Antimicrobial activity of intraurethrally administered probiotic Lactobacillus casei in a murine model of Escherichia coli urinary tract infection. Antimicrob Agents Chemother. 2001;45(6):1751-60.
  10. Hagberg L, Jodal U, Korhonen TK, Lidin-Janson G, Lindberg U, Svanborg Eden C. Adhesion, hemagglutination, and virulence of Escherichia coli causing urinary tract infections. Infect Immun. 1981;31(2):564-70.
  11. Ørskov F, Ørskov I. 2 Serotyping of Escherichia coli**The terminology used to describe the different classes of bacterial antigens is explained in the Preface. However, the authors would like to mention that a different convention is used in some laboratories, for example O:l, K:l, H:7 is equivalent to O1:K1:H7. In: Bergan T, editor. Methods in Microbiology. 14: Academic Press; 1984. p. 43-112.
  12. Hopkins WJ, Gendron-Fitzpatrick A, Balish E, Uehling DT. Time course and host responses to Escherichia coli urinary tract infection in genetically distinct mouse strains. Infect Immun. 1998;66(6):2798-802.
  13. Bolocan AS, Callanan J, Forde A, Ross P, Hill C. Phage therapy targeting Escherichia coli-a story with no end? FEMS Microbiol Lett. 2016;363(22).
  14. Letkiewicz S, Miedzybrodzki R, Klak M, Jonczyk E, Weber-Dabrowska B, Gorski A. The perspectives of the application of phage therapy in chronic bacterial prostatitis. FEMS Immunol Med Microbiol. 2010;60(2):99-112.
  15. Tothova L, Celec P, Babickova J, Gajdosova J, Al-Alami H, Kamodyova N, et al. Phage therapy of Cronobacter-induced urinary tract infection in mice. Med Sci Monit. 2011;17(7):173-8.
  16. Cafora M, Deflorian G, Forti F, Ferrari L, Binelli G, Briani F, et al. Phage therapy against Pseudomonas aeruginosa infections in a cystic fibrosis zebrafish model. Sci Rep. 2019;9(1):1527.
  1. Kelly D, McAuliffe O, Ross RP, O'Mahony J, Coffey A. Development of a broad-host-range phage cocktail for biocontrol. Bioeng Bugs. 2011;2(1):31-7.
  2. Manohar P, Tamhankar AJ, Lundborg CS, Nachimuthu R. Therapeutic Characterization and Efficacy of Bacteriophage Cocktails Infecting Escherichia coli, Klebsiella pneumoniae, and Enterobacter Species. Front Microbiol. 2019;10:574.
  3. Nasr-Eldin M, Abo EL-Maaty S, EL-Dougdoug K, Hazaa M, Abdel-mageed A. Characterization and development of a phage cocktail for Escherichia coli causing gastrointestinal diseases. J Bas Environ Sci. 2011;5: 115-22.
  1. Furfaro LL, Chang BJ, Payne MS. Applications for Bacteriophage Therapy during Pregnancy and the Perinatal Period. Front Microbiol. 2017;8:2660.
  2. Arenas-Hernandez MM, Martinez-Laguna Y, Torres AG. Clinical implications of enteroadherent Escherichia coli. Curr Gastroenterol Rep. 2012;14(5):386-94.
آمار
تعداد مشاهده مقاله: 2,370
تعداد دریافت فایل اصل مقاله: 932


counter
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب