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Khandia, R, Pattnaik, B, Rajukumar, K, Pateriya, A. K, Puranik, N, Bhatia, S, Murugkar, H. (1400). Identification of other Cellular Receptors for Edema Factor of Bacillus anthracis by Independent Inhibition of Protective Antigen Evidenced by Inhibition of Embryo Growth and Angiogenesis. سامانه مدیریت نشریات علمی, 76(4), 847-855. doi: 10.22092/ari.2021.355959.1756
R Khandia; B Pattnaik; K Rajukumar; A. K Pateriya; N Puranik; S Bhatia; H Murugkar. "Identification of other Cellular Receptors for Edema Factor of Bacillus anthracis by Independent Inhibition of Protective Antigen Evidenced by Inhibition of Embryo Growth and Angiogenesis". سامانه مدیریت نشریات علمی, 76, 4, 1400, 847-855. doi: 10.22092/ari.2021.355959.1756
Khandia, R, Pattnaik, B, Rajukumar, K, Pateriya, A. K, Puranik, N, Bhatia, S, Murugkar, H. (1400). 'Identification of other Cellular Receptors for Edema Factor of Bacillus anthracis by Independent Inhibition of Protective Antigen Evidenced by Inhibition of Embryo Growth and Angiogenesis', سامانه مدیریت نشریات علمی, 76(4), pp. 847-855. doi: 10.22092/ari.2021.355959.1756
Khandia, R, Pattnaik, B, Rajukumar, K, Pateriya, A. K, Puranik, N, Bhatia, S, Murugkar, H. Identification of other Cellular Receptors for Edema Factor of Bacillus anthracis by Independent Inhibition of Protective Antigen Evidenced by Inhibition of Embryo Growth and Angiogenesis. سامانه مدیریت نشریات علمی, 1400; 76(4): 847-855. doi: 10.22092/ari.2021.355959.1756

Identification of other Cellular Receptors for Edema Factor of Bacillus anthracis by Independent Inhibition of Protective Antigen Evidenced by Inhibition of Embryo Growth and Angiogenesis

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مقاله 14، دوره 76، شماره 4، آذر و دی 2021، صفحه 847-855    اصل مقاله (470.05 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355959.1756
نویسندگان
R Khandia* 1، 2؛ B Pattnaik3؛ K Rajukumar1؛ A. K Pateriya1؛ N Puranik2؛ S Bhatia1؛ H Murugkar1
1ICAR-National Institute for High Security Animal Diseases, Anand Nagar, Bhopal, MP, India
2Department of Biochemistry and Genetics, Barkatullah University, Bhopal, MP, India
3Project Directorate on Foot and Mouth Disease, Indian Council of Agricultural Research, Mukteswar - Kumaon, Nainital, UK
چکیده
Edema factor (EF) is one of the major secretory proteins of anthrax bacteria along with protective antigen (PA) and lethal factor (LF). Edema factor is a calmodulin-and calcium-dependent adenylate cyclase that increases intracellular levels of cAMP. Intracellular trafficking of EF occurs through PA by binding to ATR/CMG2 receptors, which are also involved in other physiological functions of cells. cAMP is a secondary messenger which activates multiple signaling cascades involved in the cytokinetics of actin molecules and cell junction formation. The present study evaluated the effect of EF on growth and angiogenesis patterns in chicken embryos in the in ovo model. Angiogenesis in the chorioallantoic membrane (CAM) of an embryonated chicken egg was decreased and embryo growth was delayed by EF despite the absence of trafficking moiety PA, which is required for transferring the EF molecule inside the cell. Angiogenesis inhibition and embryo growth retardation indicate the use of an alternative receptor by EF to modulate these cellular functions. Additionally, docking was performed between EF as a ligand and hepatocyte growth factor receptor (cMET) and vascular endothelial growth factor (VEGF) receptors, which are mainly involved in growth and angiogenesis. The analysis revealed a very strong binding of EF to cMET receptor (in terms of the number of hydrogen bonds and energy) compared to its ligand hepatocyte growth factor (HGF), which indicates the use of cMET receptor by EF and induction of angiogenesis and embryo growth retardation possibly by competitive inhibition of HGF ligand or receptor-mediated endocytosis.
کلیدواژه‌ها
Angiogenesis inhibition؛ Edema factor؛ CAM؛ Embryo growth retardation؛ cAMP؛ Signaling molecule
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مراجع
  1. Bradley KA, Mogridge J, Mourez M, Collier RJ, Young JAT. Identification of the cellular receptor for anthrax toxin. Nature. 2001;414(6860):225–9.
  2. Young JA and Collier RJ. 2007. Anthrax toxin: receptor binding, internalization, pore formation, and translocation.
    Annu Rev Biochem. 76:243-65.
  3. Cheng X, Ji Z, Tsalkova T, Mei F. Epac and PKA: a tale of two intracellular cAMP receptors. Acta biochimica et biophysica Sinica. 2008;(7):651-62.
  4. Lokker NA, Mark MR, Luis EA, Bennett GL, Robbins KA, Baker JB, et al. Structure-function analysis of hepatocyte growth factor: identification of variants that lack mitogenic activity yet retain high affinity receptor binding. EMBO J. 1992;11(7):2503-10.
  5. Tajima H, Matsumoto K, Nakamura T. 1991. Hepatocyte growth factor has potent anti-proliferative activity in various tumor cell lines. FEBS Lett. 291(2):229-32.
  6. Naldini L, Weidner KM, Vigna E, Gaudino G, Bardelli A, Ponzetto C, et al. Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J. 1991;10(10):2867.
  7. Montesano RK, Matsumoto K, Nakamura T, Orci L. Identification of a fibroblast-derived epithelial morphogen as hepatocyte growth factor. Cell. 1991;67(5):901-8.
  8. Basilico C, Arnesano A, Galluzzo M, Comoglio PM, Michieli P. A high affinity hepatocyte growth factor-binding site in the immunoglobulin-like region of Met. J Biol Chem. 2008;283(30):21267-77.
  9. Goel HL, Mercurio AM. VEGF targets the tumour cell. Nat Rev Cancer. 2013;13(12):871-82.
  10. Göttle M, Dove S, Kees F, Schlossmann J, Geduhn J, König B, Shen Y, Tang WJ, Kaever V, Seifert R. Cytidylyl and uridylyl cyclase activity of Bacillus anthracis edema factor and Bordetella pertussis CyaA. Biochemistry. 2010;49(26):5494-503.
  11. Tang S, Moayeri M, Chen Z, Harma H, Zhao J, Hu H, Purcell RH, Leppla SH, Hewlett IK. Detection of anthrax toxin by an ultrasensitive immunoassay using europium nanoparticles. Clin Vaccine Immunol.;16(3):408-13.
  12. Quinn CP, Singh Y, Klimpel KR, Leppla SH. Functional mapping of anthrax toxin lethal factor by in-frame insertion mutagenesis. J Biol Chem. 25;266(30):20124-30.
  13. Hong J, Doebele RC, Lingen MW, Quilliam LA, Tang WJ, Rosner MR. Anthrax edema toxin inhibits endothelial cell chemotaxis via Epac and Rap1. J Biol Chem. 2007;282(27):19781–7.
  1. del Valle-Pérez B, Martínez-Estrada OM, Vilaró S, Ventura F, Viñals F. cAMP inhibits TGFbeta1-induced in vitro angiogenesis. FEBS Lett. 2004;569(1-3):105-11
  2. Kim S, Bakre M, Yin H, Varner JA. Inhibition of endothelial cell survival and angiogenesis by protein kinase A. J Clin Investig. 2002;110(7):933-41.
  3. Khandia R, Bhatia S, Chanu KV, Sood R, Dhama K. Anthrax toxin receptors, functions and their possible use in therapeutics: a review. Asian J Anim Vet Adv. 2014;9(10):599-609.
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