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Naji Hasan, R, Abdal Kareem Jasim, S. (1400). Detection of Panton-Valentine leukocidin and MecA Genes in Staphylococcus aureus isolated from Iraqi Patients. سامانه مدیریت نشریات علمی, 76(4), 1054-1059. doi: 10.22092/ari.2021.355962.1751
R Naji Hasan; S Abdal Kareem Jasim. "Detection of Panton-Valentine leukocidin and MecA Genes in Staphylococcus aureus isolated from Iraqi Patients". سامانه مدیریت نشریات علمی, 76, 4, 1400, 1054-1059. doi: 10.22092/ari.2021.355962.1751
Naji Hasan, R, Abdal Kareem Jasim, S. (1400). 'Detection of Panton-Valentine leukocidin and MecA Genes in Staphylococcus aureus isolated from Iraqi Patients', سامانه مدیریت نشریات علمی, 76(4), pp. 1054-1059. doi: 10.22092/ari.2021.355962.1751
Naji Hasan, R, Abdal Kareem Jasim, S. Detection of Panton-Valentine leukocidin and MecA Genes in Staphylococcus aureus isolated from Iraqi Patients. سامانه مدیریت نشریات علمی, 1400; 76(4): 1054-1059. doi: 10.22092/ari.2021.355962.1751

Detection of Panton-Valentine leukocidin and MecA Genes in Staphylococcus aureus isolated from Iraqi Patients

Archives of Razi Institute
مقاله 35، دوره 76، شماره 4، آذر و دی 2021، صفحه 1054-1059    اصل مقاله (189.33 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355962.1751
نویسندگان
R Naji Hasan* 1؛ S Abdal Kareem Jasim2
1Biotechnology and Environmental Center, University of Fallujah, Al-Fallujah, Iraq
2Medical Laboratory Techniques Department, Al-Maarif University College, Anbar, Iraq
چکیده
A gram-positive bacterium, Staphylococcus aureus, which is widely distributed is considered as a bacterial infection that commonly infects the skin and mucous membranes. Such infections can be the cause of death and illness. In the present study  by using reverse transcription-polymerase chain reaction (rt-PCR) the Panton-Valentine leukocidin (PVL) and mecA genes of S. aureus which were isolated from skin and soft tissue infections (SSTIs) in Baghdad, Iraq were investigated. This study included 96 S. aureus isolated from SSTIs and identified by Vitek. The results showed that 61 (63.5%) and 48 (50%) of the isolates were positive for PVL and mecA genes, respectively. This work presented an effective real-time PCR technique for detecting PVL genes alone or in conjunction with mecA. The rt-PCR allows for easier reaction monitoring and eliminates the need for post-PCR processing, saving both resources and time. Moreover, it is ideal for diagnostic applications because of its high sensitivity, simplicity, and specificity. Besides, the rt-PCR has an option to do all the procedures in an automated mode of action.
کلیدواژه‌ها
MeC A gene؛ PVL gene؛ Real-Time PCR؛ Staphylococcus aureus
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مراجع
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