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| تعداد نشریات | 278 |
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| تعداد دریافت فایل اصل مقاله | 11,022,567 |
Mycosynthesis of Silver Nanoparticles by Candida albicans Yeast and Its Biological Applications | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 29 شهریور 1400 اصل مقاله (1.01 MB) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2021.355935.1741 | ||
| نویسندگان | ||
R. A Hikmet  ؛ N. N Hussein
| ||
| Department of Applied Sciences, Biotechnology Division, University of Technology, Iraq | ||
| چکیده | ||
| This research was carried out for the mycosynthesis of silver nanoparticles (AgNPs) by Candida albicans supernatant. The mycosynthesized silver nanoparticles were identified by color visualization, ultraviolet-visible (UV) spectroscopy device, X-Ray Diffraction (XRD), Energy Dispersive Analysis of X-ray (EDX), Field emission Scanning electron microscope (FESEM), and Zeta potential analysis. The UV-Vis spectroscopy examination has shown the highest absorbance (λmax) at the wavelength of 429 nanometers which indicator of the creation of silver nanoparticle, X-Ray Diffraction (XRD) has shown the crystalline structure of silver nanoparticles, Energy Dispersive Analysis of X-ray (EDX) has shown the weight percentage of silver atoms in the sample are 82.4%, Field emission Scanning electron microscope (FESEM) has shown the morphology of silver nanoparticles is spherical, and its size is 40.19 nanometer. Zeta potential analysis has shown silver nanoparticles are middling stable in the solution, and the zeta potential of silver nanoparticles mycosynthesised by Candida albicans is (− 23.02) mV. The cytotoxic effect of silver nanoparticles against a human colon cancer cell line (HT-29) usingMTTassay has shown the presence of toxic action against the cells, and no cytotoxic effect appears on the normal cells. The antioxidant activity of silver nanoparticles by using DPPH assay has shown 17.0%, 29.3%, 48.3%, 67.6%, 83.6% at concentrations (6.25, 12.5, 25, 50, 100) µg/ml, respectively. The impact of silver nanoparticles on the chromosomal pattern also has been studied. The importance of this work lies in the possibility of the synthesis of silver nanoparticles using this yeast because most nanoparticles preparation methods utilize molds. | ||
| کلیدواژه‌ها | ||
| Nanoparticles؛ AgNPs؛ Antioxidant؛ Candida albicans؛ Cytotoxicity؛ (EDX)؛ (FESEM)؛ (UV) Spectroscopy؛ (XRD)؛ Zeta Potential | ||
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آمار تعداد مشاهده مقاله: 33 تعداد دریافت فایل اصل مقاله: 24 |
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