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Agarkova, A., Tverskoi, A., Morozov, V., Tverskaya, A., Morozova, E., Mukhina, T.. (1400). Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone. سامانه مدیریت نشریات علمی, 76(4), 1005-1012. doi: 10.22092/ari.2021.355885.1733
A. A Agarkova; A. V Tverskoi; V. N Morozov; A. V Tverskaya; E. N Morozova; T. S Mukhina. "Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone". سامانه مدیریت نشریات علمی, 76, 4, 1400, 1005-1012. doi: 10.22092/ari.2021.355885.1733
Agarkova, A., Tverskoi, A., Morozov, V., Tverskaya, A., Morozova, E., Mukhina, T.. (1400). 'Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone', سامانه مدیریت نشریات علمی, 76(4), pp. 1005-1012. doi: 10.22092/ari.2021.355885.1733
Agarkova, A., Tverskoi, A., Morozov, V., Tverskaya, A., Morozova, E., Mukhina, T.. Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone. سامانه مدیریت نشریات علمی, 1400; 76(4): 1005-1012. doi: 10.22092/ari.2021.355885.1733

Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone

Archives of Razi Institute
مقاله 30، دوره 76، شماره 4، آذر و دی 2021، صفحه 1005-1012    اصل مقاله (627.28 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355885.1733
نویسندگان
A. A Agarkova؛ A. V Tverskoi* ؛ V. N Morozov؛ A. V Tverskaya؛ E. N Morozova؛ T. S Mukhina
Belgorod State University, 308015, Russia, Belgorod, Pobeda St. 85
چکیده
The soft and delicate tissue of the brain, which is the center of our coordination, is protected by its surrounding layers. The disruption of these layers results in complicated situations and serious health problems. The brain has three protective layers of bone or skull tissue, the blood tissue layer, and finally the meningeal layer. The layer of blood tissue contains the blood vessels that are located between the skull and the meningeal membranes. If germs or foreign matter enter the fluid through the blood vessels under any circumstances and cause infection, the bones that protect the meninges will break and cause tissue damage. The present study aimed to assess the histological and immunohistochemical characteristics of the brain of rats that underwent induced acute purulent pneumococcal meningitis after antibiotic therapy with Ceftriaxone. A number of 20 white adult male Wistar rats were assigned to three groups. The first group (n=5) regarded as the control were injected with a saline solution into the subarachnoid space in an equivalent amount. The second and third groups of rats (n=5 and 10, respectively) were infected with acute purulent meningitis by the injection of 10 μl of Streptococcus pneumoniae (S. pneumonia) suspension into the subarachnoid space of the brain using a 23-G needle. The various areas of the brains of rats after meningitis induced by S. pneumoniae were examined after the treatment with Ceftriaxone. The S. pneumoniae culture was injected into the subarachnoid space in the area of the rhomboid fossa. Treatment started 18 h after the injection. On day 10, a repeated puncture was performed with the analysis of cerebrospinal fluid in order to confirm the absence of meningitis; thereafter, the animals were taken out of the experiment. No signs of meningitis were found on histological examination. Mild perivascular and pericellular focal edema were revealed with signs of overload of the lymphatic system in the brain and focal ischemic changes in neurons. The investigation of expression with caspase-3 revealed a positive reaction of individual neurons. A positive reaction with antibodies to NeuN and Doublecortin was detected in most neurons; moreover, Glial fibrillary acidic protein (GFAP)-positive astrocytes and their processes were visualized in all layers of the brain substance. The reaction with neuron-specific enolase (NSE), microtubule-associated protein 2 (MAP-2), CD 31, and CD 34 was negative. Typical structure and pictures pointed to an intact brain and purulent meningitis in the first and second groups. The microscopic image and the changes revealed during immunohistochemistry by dual corticosteroid antibodies and neuronal nuclear protein were characterized by predominantly cytoplasmic and perinuclear reactions, respectively. Some neurons are positive for caspase-3 and are related to changes in the characteristic of premature aging.
کلیدواژه‌ها
Acute meningitis؛ Ceftriaxone؛ Immunohistochemical markers؛ Brain of rats
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مراجع
  1. Krukovskiĭ B, Salamov G. Morphology of pneumococcal meningitis in adults. Arkh Patol. 1989;51(1):55-7.
  2. Rusai K, Prókai Á, Szebeni B, Fekete A, Treszl A, Vannay Á, et al. Role of serum and glucocorticoid-regulated kinase-1 in the protective effects of erythropoietin during renal ischemia/reperfusion injury. Biochem Pharmacol. 2010;79(8):1173-81.
  3. Ginsberg L. Difficult and recurrent meningitis. J Neurol Neurosurg Psychiatry 2004;75(suppl 1):i16-i21.
  4. Puopolo KM. Bacterial and fungal infections. Manual Neonatal Care. 2008;6:274-300.
  5. Boyles TH, Bamford C, Bateman K, Blumberg L, Dramowski A, Karstaedt A, et al. Guidelines for the management of acute meningitis in children and adults in South Africa. South Afr J Epidemiol Infect. 2013;28(1):5-15.
  6. Griffiths MJ, McGill F, Solomon T. Management of acute meningitis. J Clin Med. 2018;18(2):164.
  7. Hoen B, Viel J, Paquot C, Gerard A, Canton P. Multivariate approach to differential diagnosis of acute meningitis. Eur J Clin Microbiol Infect Dis. 1995;14(4):267-74.
  8. Koedel U, Klein M, Pfister H-W. Modulation of brain injury as a target of adjunctive therapy in bacterial meningitis. Curr Infect Dis Rep. 2010;12(4):266-73.
  9. Parikh S, Koch M, Narayan RK. Traumatic brain injury. Int Anesthesiol Clin. 2007;45(3):119-35.
  10. Koedel U, Scheld WM, Pfister H-W. Pathogenesis and pathophysiology of pneumococcal meningitis. Lancet Infect Dis. 2002;2(12):721-36.
  11. Østergaard C, Konradsen HB, Samuelsson S. Clinical presentation and prognostic factors of Streptococcus pneumonia e meningitis according to the focus of infection. BMC Infect Dis. 2005;5(1):1-11.
  12. Agrawal S, Nadel S. Acute bacterial meningitis in infants and children. Pediatric Drugs. 2011;13(6):385-400.
  13. Sáez-Llorens X, McCracken Jr GH. Bacterial meningitis in children. Lancet. 2003;361(9375):2139-48.
  14. Kastenbauer S, Pfister HW. Pneumococcal meningitis in adults: spectrum of complications and prognostic factors in a series of 87 cases. Brain. 2003;126(5):1015-25.
  15. Davison K, Ramsay M. The epidemiology of acute meningitis in children in England and Wales. Arch Dis Childh. 2003;88(8):662-4.
  16. Theodoridou MN, Vasilopoulou VA, Atsali EE, Pangalis AM, Mostrou GJ, Syriopoulou VP, et al. Meningitis registry of hospitalized cases in children: epidemiological patterns of acute bacterial meningitis throughout a 32-year period. BMC Infect Dis. 2007;7(1):1-12.
  17. Gach O, Lancellotti P, Pierard LA. Acute ST-segment elevation in Neisseria meningitis. Acta Cardiologica. 2001;56(5):327-9.
  18. McGill F, Heyderman R, Michael B, Defres S, Beeching N, Borrow R, et al. The UK joint specialist societies guideline on the diagnosis and management of acute meningitis and meningococcal sepsis in immunocompetent adults. J Infect. 2016;72(4):405-38.
  19. Schaad UB. Arthritis in disease due to Neisseria meningitidis. Rev Infect Dis. 1980;2(6):880-8.
  20. Bartt R. Acute bacterial and viral meningitis. Lifelong Learn Neurol. 2012;18(6):1255-70.
  21. Chadwick DR. Viral meningitis. Br Med Bull. 2005;75(1):1-14.
  22. Ellner JJ, Bennett JE. Chronic meningitis. Medicine. 1976;55(5):341-69.
  23. Lan SH, Chang WN, Lu CH, Lui CC, Chang HW. Cerebral infarction in chronic meningitis: a comparison of tuberculous meningitis and cryptococcal meningitis. Qjm. 2001;94(5):247-53.
  24. Zunt JR, Baldwin KJ. Chronic and subacute meningitis. Lifelong Learn Neurol. 2012;18(6):1290-318.
  25. Charalambous LT, Premji A, Tybout C, Hunt A, Cutshaw D, Elsamadicy AA, et al. Prevalence, healthcare resource utilization and overall burden of fungal meningitis in the United States. J Med Microbiol. 2018;67(2):215.
  26. Gottfredsson M, Perfect JR, editors. Fungal meningitis. Seminars in neurology; 2000: Copyright© 2000 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New.
  27. Bicanic T, Harrison TS. Cryptococcal meningitis. Br Med Bull. 2004;72(1):99-118.
  28. Dismukes WE. Cryptococcal meningitis in patients with AIDS. J Infect Dis. 1988;157(4):624-8.
  29. Powderly WG, Cloud GA, Dismukes WE, Saag MS. Measurement of cryptococcal antigen in serum and cerebrospinal fluid: value in the management of AIDS-associated cryptococcal meningitis. Clin Infect Dis. 1994;18(5):789-92.
  30. Lavezzi AM, Corna MF, Matturri L. Neuronal nuclear antigen (NeuN): a useful marker of neuronal immaturity in sudden unexplained perinatal death. J Neurol Sci. 2013;329(1-2):45-50.
  31. Gusel'Nikova V, Korzhevskiy D. NeuN as a neuronal nuclear antigen and neuron differentiation marker. Acta Naturae. 2015;7(2):42-7.
  32. Korzhevskii DE, Petrova ES, Kirik OV, Beznin GV, EG S. Neural markers in investigation of stem cells differentiation. Geny Kletki. 2010;3:57-65.
  33. Zimatkin S, Bon E. Dynamics of Histological Changes in the Frontal Cortex of the Brain in Rats Subjected to Antenatal Exposure to Alcohol. Neurosci Behav Physiol. 2017;47(3):370-4.
  34. Cinzerling AV, VA. C. Modern infections. Pathological anatomy and questions of pathogenesis. . Second edition ed2002.
  35. Dolzhikov A, Tverskoi A, Bobyntsev I, Kriukov A, Belykh A. Morphometric study of hippocampal neurons in
    chronic immobilization stress. Res Results Biomed. 2015;1(4):62-5.
  36. Maksimova KY, Stefanova N, Logvinov S. Morphological changes in the hippocampus of rats in accelerated aging. Bull Sibirskoy Meditsiny. 2014;13(1):56-61.
  1. Tverskoi AV, Morozov VN, Petrenko AA, Mukhina TS, Parichuk AS. Morphological characteristics of the rats brain under hypergravity. Drug Invent Today. 2017;9:30-2.
38.          Zimatkin S, Fedina E. Histaminergic neurons of rat brain after chronic alcohol intoxication. Novosti Med-Biol Nauk. 2012(2):137-44. 

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