Evaluation of Rat’s Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxon

Agarkova, A. A; Tverskoi, A. V; Morozov, V. N; Tverskaya, A. V; Morozova, E. N; Mukhina, T. S

doi:10.22092/ari.2021.355885.1733

تعداد نشریات	278
تعداد نشریات سازمان	77
تعداد شماره‌ها	2,196
تعداد مقالات	25,430
تعداد مشاهده مقاله	13,782,533
تعداد دریافت فایل اصل مقاله	11,032,525

	Evaluation of Rat’s Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxon
Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 25 شهریور 1400 اصل مقاله (933.01 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355885.1733
نویسندگان
A. A Agarkova؛ A. V Tverskoi ؛ V. N Morozov؛ A. V Tverskaya؛ E. N Morozova؛ T. S Mukhina
Belgorod State University, 308015, Russia, Belgorod, Pobeda St. 85
چکیده
The brain, which is the center of our coordination, has protective layers around it to protect its soft and delicate tissue, and if any of these layers are disturbed, a person will face a complicated situations and serious health problems. The brain has three protective layers of bone or skull tissue, the blood tissue layer, and finally the meningeal layer. The layer of blood tissue is the blood vessels that are located between the skull and the meningeal membranes. If germs or foreign matter enter the fluid through the blood vessels in any way and cause infection, the bones that protect the meninges will break and cause tissue damage, or any other factor that can cause inflammation of these membranes can occur. The aim of the current study was to study the histological and immunohistochemical characteristics of the brain of rats that underwent induced acute purulent pneumococcal meningitis after antibiotic therapy with Ceftriaxon. Twenty white adult male Wistar rats, were divided into three groups. The first group included 5 animals as the control group. Control group were injected with a saline solution into the subarachnoid space in an equivalent amount. The second and third groups of rats, 5 and 10 animals, respectively, were simulated acute purulent meningitis by injecting 10 μl of S. pneumoniae suspension into the subarachnoid space of the brain using a 23-G needle. Various areas of the brain of rats in acute experimental meningitis caused by S. pneumoniae after treatment with Ceftriaxon were examined. The S. pneumoniae culture was injected into the subarachnoid space in the area of the rhomboid fossa. Treatment started 18 hours after the injection. On day 10, repeated puncture was performed with analysis of cerebrospinal fluid in order to confirm the absence of meningitis, after which the animals were taken out of the experiment. No signs of meningitis were found on histological examination. Mild perivascular and pericellular focal edema with signs of overload of the lymphatic system of the brain, focal ischemic changes in neurons were revealed. Investigation of expression with caspase 3 revealed a positive reaction of individual neurons. A positive reaction with antibodies to NeuN and Doublecortin was detected in most neurons, GFAP-positive astrocytes and their processes were visualized in all layers of the brain substance. The reaction with NSE, MAP-2, CD 31 and CD 34 is negative. Typical structure and pictures revealed an unchanged brain and purulent meningitis in the first and second groups. The microscopic image as well as the changes revealed during immunohistochemistry by dual corticosteroid antibodies and neuronal nuclear protein are characterized by predominantly cytoplasmic and perinuclear reactions, respectively. Some neurons are positive for caspase-3 and are related to changes in the characteristic of premature aging.
کلیدواژه‌ها
Acute meningitis؛ Ceftriaxon؛ Immunohistochemical markers؛ Rats’ brain

آمار تعداد مشاهده مقاله: 8 تعداد دریافت فایل اصل مقاله: 8

Journal Management System. Designed by sinaweb.

آمار

Evaluation of Rat’s Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxon