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Jumintono, J, Alkubaisy, S, Yánez Silva, D, Singh, K, Turki Jalil, A, Mutia Syarifah, Sh, Fakri Mustafa, Y, Mikolaychik, I, Morozova, L, Derkho, M. (1400). The Effect of Cystamine on Sperm and Antioxidant Parameters of Ram Semen Stored at 4 °C for 50 Hours. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2021.355901.1735
J Jumintono; S Alkubaisy; D Yánez Silva; K Singh; A Turki Jalil; Sh Mutia Syarifah; Y Fakri Mustafa; I Mikolaychik; L Morozova; M Derkho. "The Effect of Cystamine on Sperm and Antioxidant Parameters of Ram Semen Stored at 4 °C for 50 Hours". سامانه مدیریت نشریات علمی, , , 1400, -. doi: 10.22092/ari.2021.355901.1735
Jumintono, J, Alkubaisy, S, Yánez Silva, D, Singh, K, Turki Jalil, A, Mutia Syarifah, Sh, Fakri Mustafa, Y, Mikolaychik, I, Morozova, L, Derkho, M. (1400). 'The Effect of Cystamine on Sperm and Antioxidant Parameters of Ram Semen Stored at 4 °C for 50 Hours', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2021.355901.1735
Jumintono, J, Alkubaisy, S, Yánez Silva, D, Singh, K, Turki Jalil, A, Mutia Syarifah, Sh, Fakri Mustafa, Y, Mikolaychik, I, Morozova, L, Derkho, M. The Effect of Cystamine on Sperm and Antioxidant Parameters of Ram Semen Stored at 4 °C for 50 Hours. سامانه مدیریت نشریات علمی, 1400; (): -. doi: 10.22092/ari.2021.355901.1735

The Effect of Cystamine on Sperm and Antioxidant Parameters of Ram Semen Stored at 4 °C for 50 Hours

Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 24 شهریور 1400  XML اصل مقاله (553.21 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355901.1735
نویسندگان
J Jumintono email 1؛ S Alkubaisy2؛ D Yánez Silva3؛ K Singh4؛ A Turki Jalil5، 6؛ Sh Mutia Syarifah7؛ Y Fakri Mustafa8؛ I Mikolaychik9؛ L Morozova9؛ M Derkho10
1Faculty of Technical and Vocational Education, Universiti Tun Hussein Onn Malaysia, 86400, Johor, Malaysia
2University of Anbar – Center of Desert Studies,11, Ramadi, Iraq
3Universidad Estatal de Bolivar, Facultad de CienciasAgropecuarias, Carrera de MedicinaVeterinaria; CP: 020150, Guaranda-Ecuador
4GLA University Mathura-India
5Faculty of Biology and Ecology, YankaKupala State University of Grodno, 230023 Grodno, Belarus
6College of technical engineering, The Islamic University, Najaf, Iraq
7Department of Chemical Engineering Technology, Faculty of Engineering Technology, University of Tun Hussein Onn Malaysia, 86400, Johor, Malaysia
8Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul-41001, Iraq
9Kurgan State Agricultural Academy by T.S. Maltsev, Lesnikovo village, Ketovsky district, Kurgan region, 641300 Russian Federation
10South-Urals State Agrarian University, 13 Gagarin St., Troitsk, Chelyabinsk region, 457100, Russian Federation
چکیده
Physical and chemical changes in the spermatozoa membrane caused by oxidative stress can reduce spermatozoa function and even death. Cystamine (NH2-CH2-CH2-SH, β-mercaptoethylamine) is one of the natural substances that modulate the endocrine and metabolic status of animals. This substance has antioxidant and anti-apoptotic effects by inducing intracellular cysteine accumulation. Cystamine is used to treat many diseases but it has many side effects. Sheep semen is sensitive to the stressful condition of chilling storage, which restricts semen storage in commercial flocks for artificial insemination. The effect of cystamine on spermatogenesis is not yet fully understood. This study was aimed to investigate the effect of cysteamine addition to the sheep sperm extender during cooling storage on the semen quality parameters. Sperm samples from six Edilbayevskaya rams (2 and 3 years old, 70-85 kg) were used in this investigation. The samples were diluted by the extender and supplemented with different concentrations of cysteamine (0, 1, 2, 5, and 10 mM) and were cooled to 4ºC for 50 h. Motility parameters, membrane integrity, viability, lipid peroxidation, and mitochondria activity of cooled semen were evaluated at 0, 25, and 50 h of cooling storage. Although at the start time (0 hrs), cysteamine did not affect semen quality, extender supplementation with cysteamine improved sperm total motility, progressive motility and mitochondria membrane potential during storage periods (P≤0.01). Moreover, using 1 and 2 mM cysteamine improved (P≤0.01) sperm membrane functionality and viability compared to other treatments. Antioxidant potential (AOP), lipid peroxidation (LPO), and total glutathione (tGSH) (except AOP at 50 h) were significantly different after semen storage at 4 °C. Therefore, with the use of cysteamine levels of AOP and tGSH increased significantly. Supplementing cysteamine (1 and 2 mM cysteamine) lead to lower levels of LPO (p<0.01) at 0, 25, and 50 h. Therefore, finding and using the best concentrations of cysteamine to the cooling extender could be effective to save sheep semen against damages of the cooling storage process.
کلیدواژه‌ها
Cooling؛ Cysteamin؛ Semen؛ Sheep
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