| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,139 |
| تعداد مقالات | 34,918 |
| تعداد مشاهده مقاله | 50,383,394 |
| تعداد دریافت فایل اصل مقاله | 90,276,782 |
مقایسه آثار اسانس دو گونه گیاه Origanum vulgare L. و Origanum majorana L. بر رده سلول سرطانی BCL-1 و سلولهای سیستم ایمنی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 6، دوره 37، شماره 5 - شماره پیاپی 109، آذر و دی 1400، صفحه 781-794 اصل مقاله (941.64 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2021.352596.2898 | ||
| نویسندگان | ||
| فاطمه مشهدی1؛ مجید قربانی نهوجی2؛ رویا یارایی* 3 | ||
| 1دانشجوی کارشناسی ارشد، گروه ایمونولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران | ||
| 2استادیار، سیستماتیک گیاهی، مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاد دانشگاهی، کرج، ایران | ||
| 3دانشیار، گروه ایمونولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران | ||
| چکیده | ||
| گیاهان خانواده نعناعیان منبع غنی از ترکیبهای دارویی با خواص ضدسرطان هستند. در بسیاری از تحقیقات تنها به خواص ضد سرطانی این ترکیبها پرداخته شده است و به سمّیت آنها بر سلولهای سالم توجهی نشده است. این مطالعه با هدف ارزیابی و مقایسه اثرهای سمّی اسانس دو گونه از جنس مرزنجوش (خانواده نعناعیان) شامل Origanum vulagare L. و Origanum majorana L. بر رده سلول سرطانی BCL-1 و لنفوسیت (ایمنی اکتسابی) و ماکروفاژ (ایمنی ذاتی) نرمال در شرایط In vitro طراحی شده است. نمونه گیاهان تهیه و اسانس روغنی با روش تقطیر با آب آماده شد. سپس آنالیز اسانسها با دستگاههای GC و GC.MS انجام گردید. رده سلول سرطانی BCL-1، لنفوسیت و ماکروفاژ نرمال با غلظتهای مختلف اسانسها به مدت 24 ساعت کشت داده شد. حیات سلولی و مسمومیت سلولی تحت تأثیر اسانسهای روغنی از طریق سنجش MTT و LDH تعیین شد. نتایج GC.MS تنوع ترکیبهای دو اسانس را نشان میدهد، بهطوری که تنها دارای 12 جزء مشترک میباشد. دو اسانس در محدوده غلظت 1-01/0 درصد (O. vulgare 47-79% و O. majorana 30-82%) موجب مهار رشد سلول سرطانی BCL-1 میشود (IC50 اسانس O. vulgare: 01/0% و IC50 اسانس O. majorana: 03/0%). هر دو اسانس در بالاترین غلظت (1%) فعالیت حیاتی سلولهای ایمنی را کاهش نمیدهند. همچنین با توجه به نتایج آزمون LDH هیچیک از غلظتهای مورد آزمایش دو اسانس اثر سمّی بر لنفوسیت و ماکروفاژ نداشتند. اسانس گیاهان O. vulgare و O. majorana کاندید مناسبی برای درمان سرطان میباشند، زیرا علاوهبر داشتن خاصیت ضدسرطانی قوی، در سلولهای نرمال سیستم ایمنی آسیبی ایجاد نمیکنند. | ||
| کلیدواژهها | ||
| Origanum؛ ضدسرطان؛ BCL-1؛ لنفوسیت؛ ماکروفاژ | ||
| عنوان مقاله [English] | ||
| Effects of essential oils of Origanum vulgare L. and Origanum majorana L. on cancer cells line BCL-1 and immune system cells | ||
| نویسندگان [English] | ||
| F. Mashhadi1؛ M. Ghorbani Nohooji2؛ R. Yaraee3 | ||
| 1M.Sc. student, Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran | ||
| 2Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran | ||
| 3Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran | ||
| چکیده [English] | ||
| Plants from the fam. lamiaceae are the rich sources of medicinal compounds with the anticancer properties. In many studies, only the anticancer properties of these compounds have been investigated and their toxicity on the healthy cells has not been addressed. The present study was designed to evaluate and compare the toxic effects of Origanum vulgare L. and O. majorana L. essential oils on the cancer cells line BCL-1 and normal lymphocytes and macrophages under in vitro conditions. The plant samples essential oils were extracted using the hydro-distillation method and analyzed by GC and GC/MS. The cancer cells line BCL-1 and normal lymphocytes and macrophages were cultured with the various concentrations of essential oils for 24h. The viability and cells toxicity under the influence of essential oils were determined by the MTT and LDH assays. The GC/MS results showed that the two essential oils had only 12 common components. Both essential oils inhibited the growth of cancer cells line BCL-1 (O. vulgare, 47-79% and O. majorana, 30-82%) at a concentration of 0.01-1% (O. vulgare essential oil IC50: 0.01% and O. majorana essential oil IC50: 0.03%). The essential oils at the highest concentration (1%) did not decrease the immune cells viability. Also, according to the LDH test results, none of the concentrations of both essential oils had a toxic effect on the normal lymphocytes and macrophages. Overall, based on the present study results, the O. vulgare and O. majorana essential oils could be recommended as good candidates for the cancer treatment because they had no cytotoxic effect on the normal immune system cells in addition to having the strong anticancer properties. | ||
| کلیدواژهها [English] | ||
| Origanum, anti-cancer, BCL-1, lymphocyte, macrophage | ||
| مراجع | ||
|
- Adams, R.P., 2007. Identification of Essential Oils Components by Gas Chromatography/Quadrupole Mass Spectroscopy. Allured Publishing Corporation, Carol Stream, IL, USA, 804p. - Arvin, A., Campadelli-Fiume, G., Mocarski, E., Moore, P.S., Roizman, B., Whitley, R. and Yamanishi, K., 2007. Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis. Cambridge University Press, 1408p. - Begnini, K.R., Nedel, F., Lund, R.G., Carvalho, P.H.A., Rodrigues, M.R.A., Beira, F.T.A. and Del-Pino, F.A.B., 2014. Composition and antiproliferative effect of essential oil of Origanum vulgare against tumor cell lines. Journal of Medicinal Food, 17(10): 1129-1133. - Bomken, S., van der Werff Ten Bosch, J., Attarbaschi, A., Bacon, C.M., Borkhardt, A., Boztug, K., Fischer, U., Hauck, F., Kuiper, R.P., Lammens, T., Loeffen, J., Neven, B., Pan-Hammarstrom, Q., Quinti, I., Seidel, M.G., Warnatz, K., Wehr, C., Lankester, A.C. and Gennery, A.R., 2018. Current understanding and future research priorities in malignancy associated with inborn errors of immunity and DNA repair disorders: the perspective of an interdisciplinary working group. Frontiers in Immunology, 9: 2912. - Doyle, A., Stein, M. and Keshav, S., 1995. Assay for macrophage activation by cytokine. In: Balkwill, F.R., (Ed.). Cytokines-a Practical Approach. IRL Press, Oxford, 384p. - Dranoff, G., 2004. Cytokines in cancer pathogenesis and cancer therapy. Nature Reviews Cancer, 4: 11-22. - Elansary, H.O., Abdelgaleil, S.A.M., Mahmoud, E.A., Yessoufou, K., Elhindi, L. and El-Hendawy, S., 2018. Effective antioxidant, antimicrobial and anticancer activities of essential oils of horticultural aromatic crops in northern Egypt. BMC Complementary and Alternative Medicine, 18: 214. - Ghasemi Dehkordi, N.A., Sajadi, S.E., Ghanadi, A.R., Amanzadeh, Y., Azadbakht, M., Asghari, G.R., Amin, Gh.R., Haji Akhoundi, A. and Taleb, A.M., 2003. Iranian herbal pharmacopoeia (IHP). Hakim Research Journal, 6: 63-69. - Gonzalez, H., Hagerling, C. and Werb, Z., 2018. Roles of the immune system in cancer: from tumor initiation to metastatic progression. Genes & Development, 32(19-20): 1267-1284. - Gu, Y., Ting, Z., Qiu, X., Zhang, X., Gan, X., Fang, Y., Xu, X. and Xu, R., 2010. Linalool preferentially induces robust apoptosis of a variety of leukemia cells via upregulating p53 and cyclin-dependent kinase inhibitors. Toxicology, 268: 19-24. - Han, X. and Parker, T.L., 2017. Anti-inflammatory, tissue remodeling, immunomodulatory, and anticancer activities of oregano (Origanum vulgare) essential oil in a human skin disease model', Biochimie Open, 4: 73-77. - Hashemi-Bahremani, M., Parwaresch, M.R., Tabrizchi, H., Gupta, R.K. and Raffii, M.R., 2007. Lymphomas in Iran. Archives of Iranian Medicine, 10(3): 343-348. - Iwasaki, K., Zheng, Y.W., Murata, S., Ito, H., Nakayama, K., Kurokawa, T., Sano, N., Nowatari, T., Villareal, M.O., Nagano, Y.N., Isoda, H., Matsui, H. and Ohkohchi, N., 2016. Anticancer effect of linalool via cancer-specific hydroxyl radical generation in human colon cancer. World Journal of Gastroenterology, 22(44): 9765-9774. - Jeddi, S., Jafarpour, S.A., Yeganeh, S. and Naseri, M., 2018. Evaluation of color and tissue of rainbow trout fillet by chitosan edible coating incorporated with marjoram essential oil during refrigerated storage. Fisheries Science and Technology, 7: 33-39. - Leyva-López, N., Gutiérrez-Grijalva, E.P., Vazquez-Olivo, G. and Heredia, J.B., 2017. Essential oils of oregano: biological activity beyond their antimicrobial properties. Molecules, 22: 989. - Luu, S., Gardiner, E.E. and Andrews, R.K., 2018. Bone marrow defects and platelet function: a focus on MDS and CLL. Cancers, 10(5): 147. - McLafferty, F.W. and Stauffer, D.B., 1989. The Wiley/NBS Registry of Mass Spectral Data. Wiley, 1064p. - Mombeini, T., Mombeini, M. and Aghayi, M., 2009. Evaluation of pharmacological effects of Origanum genus (Origanum spp.). Journal of Medicinal Plants, 8(29): 18-189. - Morshedloo, M.R., Ahmadi, H., Pirali Hamedani, M. and Yazdani, D., 2018. An over review to Origanum vulgare L. and its pharmacological propertis. Journal of Medicinal Plants, 17(68): 15-31. - Nakayama, K., Murata, S., Ito, H., Iwasaki, K., Villareal, M.O., Zheng, Y.W., Matsui, H., Isoda, H. and Ohkohchi, N., 2017. Terpinen-4-ol inhibits colorectal cancer growth via reactive oxygen species. Oncology Letters, 14: 2015-2024. - Noori, S., Rahchamani, R., Kohsar, J.B. and Binabaj, F.B., 2015. Antibacterial effect of Lavandula stoechas and Origanum majorana essential oils against Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli. Iranian Journal of Veterinary Science and Technology, 11: 35-41. - Pozzolini, M., Scarfı̀, S., Benatti, U. and Giovine, M., 2003. Interference in MTT cell viability assay in activated macrophage cell line. Analytical Biochemistry, 313(2): 338-341. - Rahimi, A. and Farrokhi, E., 2019. Evaluation of the effect of different drying methods on antioxidant and phytochemical activity of essential oil of Origanum vulgare L. subsp. gracile. Eco-phytochemical Journal of Medicinal Plants, 7(3): 14-27. - Rubio, C.A. and Schmidt, P.T., 2018. Severe defects in the macrophage barrier to gut microflora in inflammatory bowel disease and colon cancer. Anticancer Reseaech, 38(7): 3811-3815. - Schiller, M., Metze, D., Luger, T.A., Grabbe, S. and Gunzer, M., 2006. Immune response modifiers-mode of action. Experimental Dermatology, 15: 331-341. - Shapira, Sh., Pleban, Sh., Kazanov, D., Tirosh, P. and Arber, N., 2016. Terpinen-4-ol: a novel and promising therapeutic agent for human gastrointestinal cancers. PloS One, 11(6): e0156540. - Sigvardsson, M., 2018. Molecular regulation of differentiation in early B-lymphocyte development. International Journal of Molecular Sciences, 19(7): 1928. - Sotoudeh, E., Mardani, F., Jafari, M., Habibi, H. and Moradyan, S.H., 2018. Growth indices, feeding efficiency and survival of rainbow trout (Oncorhynchus mykiss) fed diets containing different levels of Echinacea angustifolia and Origanum majorana extracts. Journal of Aquatic Animals Nutrition, 4: 1-12. - Stockinger, B., Veldhoen, M. and Martin, B., 2007. Th17 T cells: linking innate and adaptive immunity. Seminars in Immunology 19(6): 353-361. - Teixeira, B., Marques, A., Ramos, C., Serrano, C., Matos, O., Neng, N.R., Nogueira, J.M., Saraiva, J.A. and Nunes, M.L., 2013. Chemical composition and bioactivity of different oregano (Origanum vulgare) extracts and essential oil. Journal of the Science of Food and Agriculture, 93(11): 2707-2714. - Xaus, J., Comalada, M., Valledor, A.F., Cardó, M., Herrero, C., Soler, C., Lloberas, J. and Celada, A., 2001. Molecular mechanisms involved in macrophage survival, proliferation, activation or apoptosis. Immunobiology, 204(5): 543-550. - Yang, B., Chi, X.H., Lu, X.C., Tuo, S., Zhang, F., Zhang, W.Y., Tuo, C.W., Han, W.D. and Yao, S.Q., 2012. Research progress on immunocyte senescence-review. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 20(3): 782-787. - Yaraee, R., Ghazanfari, T., Eghtedardoost, M., Rajabi, M. and Naseri, M., 2011. The effect of MS14 on innate and cellular immune responses in BALB/c mice. Immunopharmacology and Immunotoxicology, 33(3): 509-514. - Yaraee, R., Kamalinejad, M., Radjabian, T., Eghtedardoost, M. and Jalali, D., 2016. Comparison of toxic effects of SIM5 and its fractions on normal resting, activated and cancerous cells. Daneshvar Medicine, 24: 1-12. - Zhang, Q.W., Lin, L.G. and Ye, W.C., 2018. Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine, 13: 1-26. | ||
|
آمار تعداد مشاهده مقاله: 1,626 تعداد دریافت فایل اصل مقاله: 514 |
||