اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,829
تعداد مقالات32,098
تعداد مشاهده مقاله40,864,089
تعداد دریافت فایل اصل مقاله18,113,716
Sabah Younus, N, Abdul-munim sharba, Z, Fakhry Altaee, M. (1400). Gene Expression of miRNAs Let-7aAssociated with Diabetes in Iraqi Population. سامانه مدیریت نشریات علمی, 76(4), 1077-1085. doi: 10.22092/ari.2021.355734.1713
N Sabah Younus; Z Abdul-munim sharba; M Fakhry Altaee. "Gene Expression of miRNAs Let-7aAssociated with Diabetes in Iraqi Population". سامانه مدیریت نشریات علمی, 76, 4, 1400, 1077-1085. doi: 10.22092/ari.2021.355734.1713
Sabah Younus, N, Abdul-munim sharba, Z, Fakhry Altaee, M. (1400). 'Gene Expression of miRNAs Let-7aAssociated with Diabetes in Iraqi Population', سامانه مدیریت نشریات علمی, 76(4), pp. 1077-1085. doi: 10.22092/ari.2021.355734.1713
Sabah Younus, N, Abdul-munim sharba, Z, Fakhry Altaee, M. Gene Expression of miRNAs Let-7aAssociated with Diabetes in Iraqi Population. سامانه مدیریت نشریات علمی, 1400; 76(4): 1077-1085. doi: 10.22092/ari.2021.355734.1713

Gene Expression of miRNAs Let-7aAssociated with Diabetes in Iraqi Population

Archives of Razi Institute
مقاله 38، دوره 76، شماره 4، آذر و دی 2021، صفحه 1077-1085    اصل مقاله (673.1 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355734.1713
نویسندگان
N Sabah Younus1؛ Z Abdul-munim sharba* 1؛ M Fakhry Altaee2
1Division of Biotechnology, Department of Applied Science, University of Technology, Baghdad, Iraq
2Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq
چکیده
miRNAs regulate protein abundance and control diverse aspects of cellular processes and biological functions in metabolic diseases, such as obesity and diabetes. Lethal-7(Let-7) miRNAs specifically target genes associated with diabetes and have a role in the regulation of peripheral glucose metabolism. The present study aimed to describe the gene expressions of the let-7a gene with the development of diabetes in Iraq and the difference in the expression of this gene in patients with diabetes and healthy individuals. The association between age and gender with the development of diabetes was studied in this study and the results were compared with those of healthy individuals in the group of control. Based on the obtained results, there was a lack in the mean of gene expression level (ΔCt) in patients, compared to controls. Moreover, the gene expression folding (2-∆∆Ct) of the let-7a reflects significant differences in terms of gene expression between groups of patients and controls, and the level of let-7a expression was reported to be 12.97 in patients with diabetes. On the other hand, significant difference was observed in terms of age and gender between diabetic patients and controls. The findings suggest that diabetes can affect individuals in all age groups and occur regardless of gender in both males and females. Based on the obtained results in this study, the gene expression level of miRNA let-7a was lower in diabetic patients compared to healthy individuals in the group of control. This also reflects differences in the gene expression fold (2-∆∆Ct) of gene let-7a between both groups of patients and controls.
کلیدواژه‌ها
Gene expression؛ Diabetes؛ Micrornalet-7a
سایر فایل های مرتبط با مقاله
مراجع
  1. Atlas D. International diabetes federation. IDF Diabetes Atlas, 7th ed. Brussels, Belgium: International Diabetes Federation. 2015.
  2. Kohei K. Pathophysiology of type 2 diabetes and its treatment policy. JMAJ. 2010;53(1):41-6.
  3. Schaffler A, Muller-Ladner U, Scholmerich J, Buchler C. Role of adipose tissue as an inflammatory organ in human diseases. Endocr Rev. 2006;27(5):449-67.
  4. Wilfred BR, Wang W-X, Nelson PT. Energizing miRNA research: a review of the role of miRNAs in lipid metabolism, with a prediction that miR-103/107 regulates human metabolic pathways. Mol Genet Metab Mol. 2007;91(3):209-17.
  5. Hammond SM. An overview of microRNAs. Adv Drug Deliv Rev. 2015;87:3-14.
  6. Arroyo JD, Chevillet JR, Kroh EM, Ruf IK, Pritchard CC, Gibson DF, et al. Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci. 2011;108(12):5003-8.
  7. Chen X, Ba Y, Ma L, Cai X, Yin Y, Wang K, et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 2008;18(10):997-1006.
  8. Cogswell JP, Ward J, Taylor IA, Waters M, Shi Y, Cannon B, et al. Identification of miRNA changes in Alzheimer's disease brain and CSF yields putative biomarkers and insights into disease pathways. J Alzheimers Dis. 2008;14(1):27-41.
  9. Gallo A, Tandon M, Alevizos I, Illei GG. The majority of microRNAs detectable in serum and saliva is concentrated in exosomes. PloS one. 2012;7(3):e30679.
  10. Zhou Q, Li M, Wang X, Li Q, Wang T, Zhu Q, et al. Immune-related microRNAs are abundant in breast milk exosomes. Int J Biol Sci. 2012;8(1):118.
  11. Weber JA, Baxter DH, Zhang S, Huang DY, How Huang K, Jen Lee M, et al. The microRNA spectrum in 12 body fluids. Clin Chem. 2010;56(11):1733-41.
  12. da Silveira JC, Veeramachaneni DR, Winger QA, Carnevale EM, Bouma GJ. Cell-secreted vesicles in equine ovarian follicular fluid contain miRNAs and proteins: a possible new form of cell communication within the ovarian follicle. Biol Reprod. 2012;86(3):71, 1-10.
  13. Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, et al. Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci 2008;105(30):10513-8.
  14. Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, et al. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature. 2000;403(6772):901-6.
  15. Dubinsky AN, Dastidar SG, Hsu CL, Zahra R, Djakovic SN, Duarte S, et al. Let-7 coordinately suppresses components of the amino acid sensing pathway to repress mTORC1 and induce autophagy. Cell Metab. 2014;20(4):626-38.
  16. Frost RJ, Olson EN. Control of glucose homeostasis and insulin sensitivity by the Let-7 family of microRNAs. Proc Natl Acad Sci 2011;108(52):21075-80.
  17. Nguyen LH, Zhu H. Lin28 and let-7 in cell metabolism and cancer. Transl Pediatr. 2015;4(1):4.
  18. Rottiers V, Najafi-Shoushtari SH, Kristo F, Gurumurthy S, Zhong L, Li Y, et al., editors. MicroRNAs in metabolism and metabolic diseases. Cold Spring Harbor symposia on quantitative biology; 2011: Cold Spring Harbor Laboratory Press.
  19. Hartig SM, Hamilton MP, Bader DA, McGuire SE. The miRNA interactome in metabolic homeostasis. Trends Endocrinol Metab. 2015;26(12):733-45.
  20. Deiuliis J. MicroRNAs as regulators of metabolic disease: pathophysiologic significance and emerging role as biomarkers and therapeutics. Int J Obes. 2016;40(1):88-101.
  21. Al-Khafaji H. Gene Expression Bcl-2 Gene in Cancer of Brest in Iraqi population. Eng Technol J. 2017;35(2 Part B):119-25.
  22. Abed SA, Al-Khafaji HM. Design Of Primer And Probe To Detect SNP Rs 1892901 In Fosl-1Gene In Different Types Of Cancer In Iraqi Population. Syst Rev Pharm. 2021;12(1):296-300.
  23. Cary N. Statistical analysis system, User's guide. Statistical. Version 9. SAS Inst Inc USA. 2012.
  1. Mohammed WH. Effect of Honey on Mice in Vitro Fertilization (IVF). Eng Technol J. 2014;32(1):107-13.
  2. Santovito D, De Nardis V, Marcantonio P, Mandolini C, Paganelli C, Vitale E, et al. Plasma exosome microRNA profiling unravels a new potential modulator of adiponectin pathway in diabetes: effect of glycemic control. J Clin Endocrinol Metab. 2014;99(9):E1681-5.
26.          Calderari S, Diawara MR, Garaud A, Gauguier D. Biological roles of microRNAs in the control of insulin secretion and action. Physiol Genomics. 2017;49(1):1-10. 

آمار
تعداد مشاهده مقاله: 1,183
تعداد دریافت فایل اصل مقاله: 387


counter
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب