Evaluation of New Immunohistochemical Approaches for the Study of Kidney Tumors in Geriatric

V. Pavlova, T; B. Pilkevich, N; V. Bessmertnyi, D; A. Pavlov, I; V. Atiakshin, D; A. Pavlova, L

doi:10.22092/ari.2021.355858.1731

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	Evaluation of New Immunohistochemical Approaches for the Study of Kidney Tumors in Geriatric
Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 18 شهریور 1400 اصل مقاله (456.99 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355858.1731
نویسندگان
T V. Pavlova ¹؛ N B. Pilkevich¹؛ D V. Bessmertnyi¹؛ I A. Pavlov²؛ D V. Atiakshin³؛ L A. Pavlova¹
¹Belgorod State University, Department of Pathology, Pobedy str., 85, 308015, Belgorod, Russian Federation
²Belgorod Cancer Clinic, urological department, Kuibysheva str., 1, 308010, Belgorod, Russian Federation
³Voronezh state medical Academy named after N. N. Burdenko, 394036, Voronezh, Studentskaya str., 10
چکیده
Kidney malignancies are one of the most deadly genitourinary tumors. It is more common in men and is often seen in people aged 70-60 years. The incidence of kidney cancer seems to be increasing. One reason for this may be the fact that imaging techniques such as computed tomography scans are more commonly used. These tests may lead to the accidental detection of more kidney cancers. Fortunately, kidney cancer is often detected in the early stages, when the cancer is small and confined to the kidney. The objective of this study was the development of new diagnostic immunohistochemical methods. Clinical examination material of 134 people, 94 (70%) men, and 40 (30%) women were used in this study. Immunohistochemical staining of tryptase in compliance with the requirements was carried out using Anti-Mast Cell Tryptase antibodies. Goat anti-mouse antibodies #AS-M1-HRP were used as secondary antibodies, visualized with ImmPACTTM DAB Peroxidase Substrate Kit (#SK-4105) according to the manufacturer's instructions. The nuclei were counterstained with Mayer's hematoxylin, and the sections were embedded in a permanent mounting medium. The immunohistochemical study showed an increase in both tryptase- and chymase-positive mast cells in the renal parenchyma compared with the control group. The number of mast cells with tryptase expression directly in the tumor was significantly less than the peritumoral localization. A similar pattern was observed for chymase-positive mast cells the content of the tumor was more than ten times higher than the intratumoral arrangement. The histological and immunological characteristics did not differ in different age groups. The immunohistochemical method of research in the diagnosis of renal tumors plays an important diagnostic and prognostic value. It can assist pathologists in difficult and ambiguous cases to correctly diagnose renal tumors. This will make it possible to prescribe the correct treatment and predict the course of malignant tumor growth in patients.
کلیدواژه‌ها
Kidneys؛ metastases؛ oncourology؛ trace elements

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Evaluation of New Immunohistochemical Approaches for the Study of Kidney Tumors in Geriatric