اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,045
تعداد مقالات34,064
تعداد مشاهده مقاله46,477,837
تعداد دریافت فایل اصل مقاله77,533,147
Skorkina, M. Y, Shevchenko, T. S, Taranenko, A. S, Shentseva, E. S, Zakirova, L. R. (1400). Evolution of Mechanical Stress (In-vitro) on Properties of Granulocytes in Acute Lymphoblastic Leukemia. سامانه مدیریت نشریات علمی, 76(4), 1087-1093. doi: 10.22092/ari.2021.355848.1727
M. Y Skorkina; T. S Shevchenko; A. S Taranenko; E. S Shentseva; L. R Zakirova. "Evolution of Mechanical Stress (In-vitro) on Properties of Granulocytes in Acute Lymphoblastic Leukemia". سامانه مدیریت نشریات علمی, 76, 4, 1400, 1087-1093. doi: 10.22092/ari.2021.355848.1727
Skorkina, M. Y, Shevchenko, T. S, Taranenko, A. S, Shentseva, E. S, Zakirova, L. R. (1400). 'Evolution of Mechanical Stress (In-vitro) on Properties of Granulocytes in Acute Lymphoblastic Leukemia', سامانه مدیریت نشریات علمی, 76(4), pp. 1087-1093. doi: 10.22092/ari.2021.355848.1727
Skorkina, M. Y, Shevchenko, T. S, Taranenko, A. S, Shentseva, E. S, Zakirova, L. R. Evolution of Mechanical Stress (In-vitro) on Properties of Granulocytes in Acute Lymphoblastic Leukemia. سامانه مدیریت نشریات علمی, 1400; 76(4): 1087-1093. doi: 10.22092/ari.2021.355848.1727

Evolution of Mechanical Stress (In-vitro) on Properties of Granulocytes in Acute Lymphoblastic Leukemia

Archives of Razi Institute
مقاله 39، دوره 76، شماره 4، آذر و دی 2021، صفحه 1087-1093    اصل مقاله (465.99 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355848.1727
نویسندگان
M. Y Skorkina* ؛ T. S Shevchenko؛ A. S Taranenko؛ E. S Shentseva؛ L. R Zakirova
Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
چکیده
Acute lymphoid leukemia (ALL) affects the lymph cells or lymphocytes that make up the lymph tissue and prevents the proper maturation of the bone marrow cells.  The processes through which cells convert mechanical stimuli into biochemical signals are called mechanical transitions and result in the sensation of specific cellular responses. In the present study, the functional properties of granulocytes of the patients with ALL were investigated using the in vitro mechanical stress model. The experimental part of the work was executed using blood from patients with ALL (n=30) being treated in the Hematological Department of Belgorod Region Hospital, Belgorod, Russia. The patients were in the age range of 18-45 years. Sample blood was obtained from all the patients who underwent a standard course of chemotherapy. Blood sampling was performed using a venepuncture and collected into the vacuum tubes Vacuette K3E. Blood samples from each experimental group were divided into two groups of control and experiment. The injection model of mechanical stress was used for the experiment group in vitro. Subsequently, the adenosine triphosphate (ATP) concentration increased by 1.8 times in this group, compared with the controls. Young’s module, which numerically characterizes the rigidity of the granulocytes’ plasmalemma, decreased by 54.4% (P<0.05) under the influence of mechanical stress. The surface potential of plasmalemma was not significantly different between samples in the group of control and experiment in patients with ALL. However, the adhesive force between erythrocyte and granulocyte increased by 30.7% (P<0.05).  The osmotic load test showed an increase in the cell’s volume during incubation. The use of membrane reserve by granulocytes increased by 47% (P<0.05) at the initial seconds of incubation. The obtained results pointed to the regulatory role of ATP molecules in intercellular signaling and add to the present literature regarding the mechanisms of intercellular interaction in the microvasculature on the development of leukemia. Moreover, the obtained results can be taken into account for the development of new pharmacological immune correctors.
کلیدواژه‌ها
Adhesive properties of biomembranes؛ Atomic force microscopy؛ Granulocytes؛ Potential surface؛ Young’s module
مراجع
  1. Moradveisi B, Yazdanifard P, Fathollahpour A. Prevalence of clinical and paraclinical features of leukemia among children with acute lymphoblastic leukemia in Sanandaj Besat Hospital, 2008-2013. Scientific J Iran Blood Transfus Organ. 2017;14(4):281-8.
  2. Burnstock G. Introduction to purinergic signaling. Purinergic Signaling: Springer; 2020. p. 1-15.
  3. Junger WG. Immune cell regulation by autocrine purinergic signalling. Nat Rev Immunol. 2011;11(3):201-12.
  4. Swennen EL, Bast A, Dagnelie PC. Immunoregulatory effects of adenosine 5′‐triphosphate on cytokine release from stimulated whole blood. Eur J Immunol 2005;35(3):852-8.
  5. Burnstock G. Purinergic signalling and disorders of the central nervous system. Nat Rev Drug Discov. 2008;7(7):575-90.
  6. Sprague RS, Stephenson AH, Ellsworth ML. Red not dead: signaling in and from erythrocytes. Trends Endocrinol Metab. 2007;18(9):350-5.
  7. Denis MFL, Incicco JJ, Espelt MV, Verstraeten SV, Pignataro OP, Lazarowski ER, et al. Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes. Biochim Biophys Acta Gen Subj. 2013;1830(10):4692-707.
  8. Di Virgilio F, Vuerich M. Purinergic signaling in the immune system. Auton Neurosci. 2015;191:117-23.
  9. Lam WA, Rosenbluth MJ, Fletcher DA. Chemotherapy exposure increases leukemia cell stiffness. Blood. 2007;109(8):3505-8.
  10. Oonishi T, Sakashita K, Uyesaka N. Regulation of red blood cell filterability by Ca2+ influx and cAMP-mediated signaling pathways. Am J Physiol Cell Physiol. 1997;273(6):C1828-C34.
  11. Skorkina MY, Fedorova M, Muravyov A, Sladkova E. The use of nanomechanic sensor for studies of morphofunctional properties of lymphocytes from healthy donors and patients with chronic lymphoblastic leukemia. Bull Exp Biol Med. 2012;154(1):163-6.
  12. Sladkova E, Skorkina MY. Estimation of surface potential of lymphocytes from patients with leukemia using Kelvin probe mode. Biophysics. 2014;59(2):254-6.
  13. Skorkina MY, Shamray E, Sladkova E. Measuring of Adhesion Force in the Cell—Cell System Based on Atomic Force Microscopy Technology. Bull Exp Biol Med. 2018:1-2.
  14. Yin J, Michalick L, Tang C, Tabuchi A, Goldenberg N, Dan Q, et al. Role of transient receptor potential vanilloid 4 in neutrophil activation and acute lung injury. Am J Respir Cell Mol Biol. 2016;54(3):370-83.
  15. Sprung R, Sprague R, Spence D. Determination of ATP release from erythrocytes using microbore tubing as a model of resistance vessels in vivo. Anal Chem. 2002;74(10):2274-8.
  16. Olearczyk JJ, Stephenson AH, Lonigro AJ, Sprague RS. Heterotrimeric G protein Gi is involved in a signal transduction pathway for ATP release from erythrocytes. Am J Physiol Heart Circ Physiol 2004;286(3):H940-H5.
  17. Chen Y, Corriden R, Inoue Y, Yip L, Hashiguchi N, Zinkernagel A, et al. ATP release guides neutrophil chemotaxis via P2Y2 and A3 receptors. Science. 2006;314(5806):1792-5.
  18. Trautmann A. Extracellular ATP in the immune system: more than just a “danger signal”. Sci Signal. 2009;2(56):pe6-pe.
  19. Egan TM, Khakh BS. Contribution of calcium ions to P2X channel responses. J Neurosci. 2004;24(13):3413-20.
  20. Montalbetti N, Denis MFL, Pignataro OP, Kobatake E, Lazarowski ER, Schwarzbaum PJ. Homeostasis of extracellular ATP in human erythrocytes. J Biol Chem. 2011;286(44):38397-407.
  21. Migliorini C, Qian Y, Chen H, Brown EB, Jain RK, Munn LL. Red blood cells augment leukocyte rolling in a virtual blood vessel. Biophys J. 2002;83(4):1834-41.
22.          Lazarowski ER. Vesicular and conductive mechanisms of nucleotide release. Purinergic Signal. 2012;8(3):359-73.

آمار
تعداد مشاهده مقاله: 1,171
تعداد دریافت فایل اصل مقاله: 2,324


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب