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Phenotypic, Antibiotyping and Molecular Detection of Klebsiella Pneumoniae Isolates from Clinical Specimens in Kirkuk, Iraq | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 18 شهریور 1400 اصل مقاله (457.37 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2021.355770.1721 | ||
| نویسندگان | ||
S Ahmed Hasan  1؛ T Fakhraddin Raheem2؛ H Mohammed Abdulla2
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| 1Department of Biology, College of Education of Pure Science Kirkuk University, Iraq | ||
| 2Basic Science Department, Faculty of Dentistry, Al. Kitab University, Iraq | ||
| چکیده | ||
| K. pneumoniae is globally responsible of hospital and community acquired infections. This study is aimed to determine prevalence of K. pneumoniae and study the antibiotic resistance profile among clinical specimens at Azadi Teaching Hospital in Kirkuk, Iraq and to detect on rpoB gene for molecular identification of Klebsiella pneumoniae in comparison with phenotypic and biochemical methods. 250 clinical specimens are collected from patients in Azadi Teaching Hospital in Kirkuk\Iraq from January 2018 to May 2018. The isolates were identified by morphologic and biochemical testing. Kirby-Bauer disk diffusion method is used in antibiotics susceptibility test. 19 (7.6%) K. pneumoniae isolates were isolated from 250 clinical specimens, 5 from males (5.61%) and 14 from females (8.69%), most of them 12 (11.76%) were isolated from 10-35 years’ age group. The isolates were reported high resistance towards various types of antibiotics especially penicillins and cephalosporins. In contrast, K. pneumoniae showed very low resistance to impenem and amikacin 5.26% ,10.52% respectively. The range of multidrug resistant (MDR) K. pneumoniae isolates in this study was 100%. In gene detection all isolates in this study showed PCR product with 108 bp by K. pneumonia specific primer (rpoB). Developed antibiotic policies and regular surveillance of antibiotic susceptibility pattern may help to overcome the indiscriminate use of antibiotics a major cause of emergence of drug resistance among pathogens. | ||
| کلیدواژه‌ها | ||
| K. pneumoniae؛ Multidrug Resistance؛ Rpob Gene | ||
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آمار تعداد مشاهده مقاله: 21 تعداد دریافت فایل اصل مقاله: 11 |
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