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Al-Muhsin Al-Khayat, F, Ahmed Kalef, D, Mohammed Khashman, B. (1400). Effect of Leishmania major Infection on the Expression of TGF Beta in Murine. سامانه مدیریت نشریات علمی, 76(5), 1399-1406. doi: 10.22092/ari.2021.355744.1715
F Al-Muhsin Al-Khayat; D Ahmed Kalef; B Mohammed Khashman. "Effect of Leishmania major Infection on the Expression of TGF Beta in Murine". سامانه مدیریت نشریات علمی, 76, 5, 1400, 1399-1406. doi: 10.22092/ari.2021.355744.1715
Al-Muhsin Al-Khayat, F, Ahmed Kalef, D, Mohammed Khashman, B. (1400). 'Effect of Leishmania major Infection on the Expression of TGF Beta in Murine', سامانه مدیریت نشریات علمی, 76(5), pp. 1399-1406. doi: 10.22092/ari.2021.355744.1715
Al-Muhsin Al-Khayat, F, Ahmed Kalef, D, Mohammed Khashman, B. Effect of Leishmania major Infection on the Expression of TGF Beta in Murine. سامانه مدیریت نشریات علمی, 1400; 76(5): 1399-1406. doi: 10.22092/ari.2021.355744.1715

Effect of Leishmania major Infection on the Expression of TGF Beta in Murine

Archives of Razi Institute
مقاله 29، دوره 76، شماره 5، بهمن و اسفند 2021، صفحه 1399-1406    اصل مقاله (1.32 M)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355744.1715
نویسندگان
F Al-Muhsin Al-Khayat* 1؛ D Ahmed Kalef2؛ B Mohammed Khashman3
1Department of Basic Sciences, College of Dentistry, University of Baghdad, Baghdad, Iraq
2Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Iraq
3National Cancer Research Centre, University of Baghdad, Iraq
چکیده
Leishmania major is a protozoan parasite that causes cutaneous Leishmaniasis disease in human beings and animals. The disease is prevalent in tropical and semitropical countries and has great health importance. The present study aimed to identify the histological changes in the organs infected with L. major and to provide a sophisticated diagnostic method for infection through detecting TGF-β cytokine by immunohistochemistry technique(IHC) from October 2020 to January 2021. A total of 40 samples of paraffin blocks were used for different organs including skin, spleen, liver, kidney, and heart of male and female BALB/c mice, aged 6-8 weeks, which were previously infected subcutaneously with L. major promastigotes at a dose of 1×107 promastigotes/moues. The result indicated epidermal hyperplasia with diffuse severe lymphohistiocytic inflammatory cells infiltration in the dermis. Hyperplasia of the lymphoid follicles was observed in infected spleen and scattered polymorphonuclear cells mainly neutrophil masses with a random distribution of microgranulomas foci composed of lymphocytes and macrophages within the liver parenchyma around central veins and portal areas. The infected kidney showed aggregation of perivascular mononuclear cells (lymphocytes and macrophages) in the renal cortex. Mononuclear lymphocytes and macrophages were observed within the heart parenchyma especially around blood vessels. Additionally, evaluation of TGF-β1 expression was highly strong for skin, spleen, relatively strong for liver, heart, and weak for the kidney. In conclusion, infection was accompanied by clinical and histological changes as well as inflammatory diseases. Furthermore, the determination of TGF-β expression level depends on the diagnosis of infection. A clear understanding of immune mechanisms is essential for preventing, treating, and controlling strategies of this infection.
کلیدواژه‌ها
TGF-β؛ Leishmania major؛ Immunohistochemistry؛ Infected mice
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مراجع
  1. Desjeux P. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis. 2004;27(5):305-18.
  2. Reithinger R, Dujardin J-C, Louzir H, Pirmez C, Alexander B, Brooker S. Cutaneous leishmaniasis. Lancet Infect Dis. 2007;7(9):581-96.
  3. Breitling R, Klingner S, Callewaert N, Pietrucha R, Geyer A, Ehrlich G, et al. Non-pathogenic trypanosomatid protozoa as a platform for protein research and production. Protein Expr Purif. 2002;25(2):209-18.
  4. Liew F, O'donnell C. Immunology of leishmaniasis. Adv Parasitol. 1993;32:161-259.
  5. Mosmann TR, Sad S. The expanding universe of T-cell subsets: Th1, Th2 and more. Immunol Today. 1996;17(3):138-46.
  6. Ji J, Sun J, Soong L. Impaired expression of inflammatory cytokines and chemokines at early stages of infection with Leishmania amazonensis. Infect Immun. 2003;71(8):4278-88.
  7. Salguero FJ, Garcia-Jimenez WL, Lima I, Seifert K. Histopathological and immunohistochemical characterization of hepatic granulomas in Leishmania donovani-infected BALB/c mice: a time-course study. Parasit Vectors. 2018;11(1):1-9.
  8. Yong L. The Theory and Practice of Histological Techniques. Pathology. 1992;24(4):320.
  9. Rezaee M, Movassaghi AR, Maleki M. Immunohistochemical expression of transforming growth factor Beta-1 in canine mammary carcinomas: its relationships with histologic grading, survival rate, and recurrence. Comp Clin Path. 2017;26(3):519-24.
  10. Sundharkrishnan L, North JP. Histopathologic features of cutaneous leishmaniasis and use of CD1a staining for amastigotes in Old World and New World leishmaniasis. J Cutan Pathol. 2017;44(12):1005-11.
  11. González K, Diaz R, Ferreira AF, García V, Paz H, Calzada JE, et al. Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama. Rev Inst Med Trop Sao Paulo. 2018;60.
  12. Cangussú SD, Souza CCd, Campos CF, Vieira LQ, Afonso LCC, Arantes RME. Histopathology of Leishmania major infection: revisiting L. major histopathology in the ear dermis infection model. Mem Inst Oswaldo Cruz. 2009;104(6):918-22.
  13. Gaafar A, El Kadaro A, Theander T, Permin H, Ismail A, Kharazmi A, et al. The pathology of cutaneous leishmaniasis due to Leishmania major in Sudan. Am J Trop Med Hyg. 1995;52(5):438-42.
  14. Scott P. Development and regulation of cell-mediated immunity in experimental leishmaniasis. Immunol Res. 2003;27(2):489-98.
  15. Heinzel FP, Sadick MD, Holaday BJ, Coffman R, Locksley RM. Reciprocal expression of interferon gamma or interleukin 4 during the resolution or progression of murine leishmaniasis. Evidence for expansion of distinct helper T cell subsets. Exp Med. 1989;169(1):59-72.
  16. Noben-Trauth N, Kropf P, Müller I. Susceptibility to Leishmania major infection in interleukin-4-deficient mice. Science. 1996;271(5251):987-90.
  17. Lohrberg M, Wilting J. The lymphatic vascular system of the mouse head. Cell Tissue Res. 2016;366(3):667-77.
  18. Jurukovski V, Dabovic B, Todorovic V, Chen Y, Rifkin DB. Methods for measuring TGF-β using antibodies, cells, and mice. Fibrosis Research: Springer; 2005. p. 161-75.
  19. Taipale J, Saharinen J, Keski-Oja J. Extracellular matrix-associated transforming growth factor-β: role in cancer cell growth and invasion. Adv Cancer Res. 1998;75:87-134.
  1. Alexander J, Bryson K. T helper (h) 1/Th2 and Leishmania: paradox rather than paradigm. Immunol Lett. 2005;99(1):17-23.
  2. Sacks D, Noben-Trauth N. The immunology of susceptibility and resistance to Leishmania major in mice. Nat Rev Immunol. 2002;2(11):845-58.
  3. Rogers KA, DeKrey GK, Mbow ML, Gillespie RD, Brodskyn CI, Titus RG. Type 1 and type 2 responses to Leishmania major. FEMS Microbiol Lett. 2002;209(1):1-7.
  4. Wege AK, Florian C, Ernst W, Zimara N, Schleicher U, Hanses F, et al. Leishmania major infection in humanized mice induces systemic infection and provokes a nonprotective human immune response. PLOS Negl Trop Dis. 2012;6(7):e1741.
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