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Mehrabi, Ali, Mahmoudi, Razzagh, Khedmati Morasa, Hajar, Mosavi, Shaghayegh, Kazeminia, Masoud, Attaran Rezaei, Fatemeh, Shahsavari, Saeed, Vahidi, Roghayeh. (1401). Study of Chemical Composition, Antibacterial and Antioxidant Activity of Thyme Leaves and Stems Essential Oil. سامانه مدیریت نشریات علمی, 11(2), 253-263. doi: 10.22092/jmpb.2021.353440.1323
Ali Mehrabi; Razzagh Mahmoudi; Hajar Khedmati Morasa; Shaghayegh Mosavi; Masoud Kazeminia; Fatemeh Attaran Rezaei; Saeed Shahsavari; Roghayeh Vahidi. "Study of Chemical Composition, Antibacterial and Antioxidant Activity of Thyme Leaves and Stems Essential Oil". سامانه مدیریت نشریات علمی, 11, 2, 1401, 253-263. doi: 10.22092/jmpb.2021.353440.1323
Mehrabi, Ali, Mahmoudi, Razzagh, Khedmati Morasa, Hajar, Mosavi, Shaghayegh, Kazeminia, Masoud, Attaran Rezaei, Fatemeh, Shahsavari, Saeed, Vahidi, Roghayeh. (1401). 'Study of Chemical Composition, Antibacterial and Antioxidant Activity of Thyme Leaves and Stems Essential Oil', سامانه مدیریت نشریات علمی, 11(2), pp. 253-263. doi: 10.22092/jmpb.2021.353440.1323
Mehrabi, Ali, Mahmoudi, Razzagh, Khedmati Morasa, Hajar, Mosavi, Shaghayegh, Kazeminia, Masoud, Attaran Rezaei, Fatemeh, Shahsavari, Saeed, Vahidi, Roghayeh. Study of Chemical Composition, Antibacterial and Antioxidant Activity of Thyme Leaves and Stems Essential Oil. سامانه مدیریت نشریات علمی, 1401; 11(2): 253-263. doi: 10.22092/jmpb.2021.353440.1323

Study of Chemical Composition, Antibacterial and Antioxidant Activity of Thyme Leaves and Stems Essential Oil

Journal of Medicinal plants and By-products
دوره 11، شماره 2، آذر 2022، صفحه 253-263    اصل مقاله (1.22 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/jmpb.2021.353440.1323
نویسندگان
Ali Mehrabi1، 2؛ Razzagh Mahmoudi* 3؛ Hajar Khedmati Morasa1؛ Shaghayegh Mosavi4؛ Masoud Kazeminia5؛ Fatemeh Attaran Rezaei6؛ Saeed Shahsavari7؛ Roghayeh Vahidi7
1Food Hygiene and Safety, School of Public Health, Qazvin University of Medical Sciences, Qazvin, Iran
2School of Health, Qazvin University of Medical Sciences, Qazvin, Iran
3Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
4Faculty of Medical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran
5Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
6Department of Microbiology, Faculty of Medical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran
7Health Productions Safety Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
چکیده
Given the increase in drug resistance of microorganisms and the tendency in using medicinal plants, the present study was carried out to investigate the chemical compounds of Thymol as a constituent of essential oil and antimicrobial activity against several pathogenic bacteria and its antioxidant activity. In this study, the chemical compounds of Thymol extracted from the two parts of the plant together (the leaves and stems) were performed by making use of gas chromatography and the other gas chromatography linked to the mass spectrograph and its antibacterial activity against Ecoli, Klebsiella, Pseudomonas, Staphylococcus, Salmonella Paratyphi B, Salmonella Typhi typhus and Enterococci through Microdilution and the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC) were investigated. The antioxidant activity of essential oils was measured through the ability of essential oil compounds in decolorizing diphenyl picrilhydrazyl (DPPH) free radicals. Chemical analysis of thyme essential oil resulted in the identification of 23 (83.68%) compounds, which Thymol with 25.30% was considered as the main part of the essential oil compound. The highest inhibitory effect was imposed on Klebsiella, Escherichia coli, and Staphylococcus aureus, and the largest inhibitory zone diameter of essential oil appeared against growth S. paratyphi B. Concerning the results, total phenol was equal to 114.3 mg of gallic acid.g and IC50 of thyme was considered to be 49.94 μM/ml. The achieved results from this study declared that thyme essential oil had a suitable inhibitory effect against pathogenic bacteria and also possessed antioxidant properties. In other words, thyme was considered as an alternative to synthetic drugs and food additives.
کلیدواژه‌ها
Thymol؛ Essential oil؛ Antioxidant؛ Antibacterial
مراجع
  1. Rahmani F., Rezaeian-Doloei R., Alimoradi L. Evaluation of Phytochemical Composition of Mentha pulegium L. Essential Oil and Its Antibacterial Activity against Several Pathogenic Bacteria. Iran J Med Microbiol. 2018; 11:167-177.
  2. Cherrat L., Espina L., Bakkali M., García‐Gonzalo D., Pagán R., Laglaoui A. Chemical composition and antioxidant properties of Laurus nobilis L. and Myrtus communis L. Essential oils from Morocco and evaluation of their antimicrobial activity acting alone or in combined processes for food preservation. J Sci Food. 2014;94: 1197-1204.
  3. Murthy N., Soumya K., Srinivas K. Antibacterial Activity of Curcuma longa (Turmeric) Plant Extracts against Bacterial Wilt of Tomato Caused by Ralstoniasolanacearum. Int J Sci Res. 2015;4: 2136-2141.
  4. Elshafie H.S., Sakr S., Mang S.M., Belviso S., De Feo V., Camele I. Antimicrobial activity and chemical composition of three essential oils extracted from Mediterranean aromatic plants. J. Med. Food. 2016;19: 1096-1103.
  5. Ramachandra T., Nagarathna A. Eco-degradation, biodiversity and health. Curr Sci. 2003;85: 1368-1369.
  6. Tajkarimi M., Ibrahim S.A., Cliver D. Antimicrobial herb and spice compounds in food. Food control. 2010;21: 1199-1218.
  7. Shahnia M., Khaksar R. Antimicrobial effects and determination of minimum inhibitory concentration (MIC) methods of essential oils against pathogenic bacteria. Iranian Journal of Nutrition Sciences & Food Technology. 2013;7: 949-955.
  8. Mohammadigholami A. Study of antifungal properties and chemical composition of essential oil of Thymus kotscuyanus Boiss. & Hohen. Iranian Journal of Plant Physiology and Biochemistry. 2016;1: 52-62.
  9. Lahooji A., Mirabolfathy M., Karami-Osboo R. Effect of Zataria multiflora and Satureja hortensis essential oils, thymol and carvacrol on growth of Fusarium gramineum isolates and deoxynivalenol production. J. Plant Pathol. 2010;46: 37-50.
  10. Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. Int. J. Food Microbiol. 2004;94: 223-253.
  11. de Arauz L.J., Jozala A.F., Mazzola P.G., Penna T.C.V. Nisin biotechnological production and application: a review. Trends Food Sci Technol. 2009;20: 146-154.
  12. Oke F., Aslim B., Ozturk S., Altundag S. Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia Ten. Food Chem. 2009;112: 874-879.
  13. AbdollahiKheirabadi S., Najafipour S., Kafilzadeh F., Abdollahi A., Jafari S., Moravej A. Evaluation of Drug Resistance Pattern of Escherichia coli Strains Isolated from Fasa Vali-e-Asr Hospital Patients. J Fasa University of Medical Sci. 2013; 2:273-278.
  14. Meshkibaf M.H., Abdollahi A., Ramandi M.F., Sadati S.A., Moravvej A, Hatami S. Antibacterial effects of hydro-alcoholic extracts of Ziziphora tenuior, Teucrium polium, Barberis corcorde and Stachys inflate. Koomesh. 2010;11: 240-245.
  15. Abdollahi A., Fasihi-Ramandi M., Kouhpayeh S.A., Najafipour S., Meshkibaf M.H., Naghdi M., Ahmadi E. Antimicrobial effect of 15 medicinal plant species and their dependency on climatic conditions of growth in different geographical and ecological areas of Fars province. Zahedan J Res Med Sci. 2012; 14:34-37.
  16. Momtaz H., Dehkordi F.S., Rahimi E., Ezadi H., Arab R. Incidence of Shiga toxin-producing Escherichia coli serogroups in ruminant's meat. Meat Sci. 2013;95: 381-388.
  17. Khoshsokhan F., Babalar M., Pourmeidani A., Fatahi M. Antioxidant activity, total phenolics and oil content of some thymus kotschyanus and thymus daenensis populations. Plant Products Technology. 2015; 15:153-162.
  18. Moosavy M., Hassanzadeh P., Mohammadzadeh E., Mahmoudi R., Khatibi S., Mardani K. Antioxidant and antimicrobial activities of essential oil of Lemon (Citrus limon) peel in vitro and in a food model. J Food Qual. Hazards Control. 2017;4: 42-48.
  19. Qadir R., Farooq Anwar T.M., Shahid M., Zahoor S. Variations in chemical composition, antimicrobial and haemolytic activities of peel essential oils from three local Citrus cultivars. Pure and Applied Biology (PAB). 2018;7: 282-291.
  20. Hashemi M., Ehsani A., Jazani N.H., Aliakbarlu J., Mahmoudi R. Chemical composition and in vitro antibacterial activity of essential oil and methanol extract of Echinophora platyloba DC against some of food-borne pathogenic bacteria. Vet Res Forum. 2013; 4:123.
  21. Etehadpour M., Tavassolian I. Ecological Factors Regulate Essential Oil Yield, Percent and Compositions of Endemic Yarrow (Achillea eriophora DC.) in Southeast Iran. Int J Hort. Sci. Technol. 2019;6: 201-215.
  22. Ehsani E., Akbari N.K., Teimouri M., Ebrahimzadeh M., Khadem A. The study of chemical composition and antimicrobial activity of Juniperus horizontalis Moench. Iranian J Medicinal and Aromatic Plants. 2012;28: 509-522.
  23. Mohammadzadeh M., Mahmoudi R., Ghajarbeygi P. Evaluation of Chemical Composition and Antibacterial Properties of Froriepia subpinnta Essential Oils from Guilan Region: Before and After Flowering. J Essent. Oil-Bear. Plants. 2018;21: 1119-1127.
  24. Borugă O., Jianu C., Mişcă C., Goleţ I., Gruia A., Horhat F. Thymus vulgaris essential oil: chemical composition and antimicrobial activity. J Med Life. 2014;7: 56-60.
  25. Fadil M., Fikri-Benbrahim K., Rachiq S., Ihssane B., Lebrazi S., Chraibi M., Haloui T., Farah A. Combined treatment of Thymus vulgaris L., Rosmarinus officinalis L. and Myrtus communis L. essential oils against Salmonella typhimurium: Optimization of antibacterial activity by mixture design methodology. Eur J Pharm Biopharm. 2018;126: 211-220.
  26. Fournomiti M., Kimbaris A., Mantzourani I., Plessas S., Theodoridou I., Papaemmanouil V., Kapsiotis I., Panopoulou M, Stavropoulou E., Bezirtzoglou E.E. Antimicrobial activity of essential oils of cultivated oregano (Origanum vulgare), sage (Salvia officinalis), and thyme (Thymus vulgaris) against clinical isolates of Escherichia coli, Klebsiella oxytoca, and Klebsiella pneumoniae. Microb. Ecol. Health Dis. 2015;26: 23289.
  27. Mehran M., Hoseini H., Hatami A., Taghizade M. Investigation of Components of Seven Species of Thyme Essential Oils and Comparison of their Antioxidant Properties. J Medicinal Plants. 2016;15: 134-140.
  28. Gedikoğlu A., Sökmen M., Çivit A. Evaluation of Thymus vulgaris and Thymbra spicata essential oils and plant extracts for chemical composition, antioxidant, and antimicrobial properties. Food Sci Nutr. 2019; 7:1704-1714.
  29. Ghasemi P.A., Jahanbazi P., Enteshari S., Malekpoor F., Hamedi B. Antimicrobial activity of some Iranian medicinal plants. Arch. Biol. Sci. 2010;62: 633-641.
  30. Ramezanpour S., Ardestani F., Asadollahzadeh M.J. Combination Effects of Zataria multiflora, Laurus nobilis and Chamaemelum nobile Essences on Pathogenic E. coli and Determination of Optimum Formulation Using Fraction and Factorial Statistical Method. Iran J Med Microbiol. 2016;10: 53-62.
  31. Bouaichi A., Benkirane R., Habbadi K., Benbouazza A., Achbani E. Antibacterial activities of the essential oils from medicinal plants against the growth of Pseudomonas stanoi savastanoi causal agent of olive knot. J Agric Vet Sci. 2015;8: 41-45.
  32. Özkalp B., Sevgi F., Özcan M., Özcan M.M. The antibacterial activity of essential oil of oregano (Origanum vulgare L.). J Food Agric Environ. 2010; 8:6-8.
  33. Mohammadpour G., Majd A., Najhadsatari T., Mehrabian S., Hossinzadehkalagar A. Antibacterial and Antifungal Effects of Three Genus of Thyme Plants and Two Ecotype of Ziziphora and Satureja Bachtiarica Essential Oils. J Sci (Islamic Azad University). 2011;20: 11-120.
  34. Rota M.C., Herrera A., Martínez R.M., Sotomayor J.A., Jordán M.J. Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils. Food control 2008;19: 681-687.
  35. Langeveld W.T., Veldhuizen E.J., Burt S.A. Synergy between essential oil components and antibiotics: a review. Crit Rev Microbiol. 2014;40: 76-94.
  36. Zangiabadi M., Sahari M., Barzegar M., Naghdi Badi H. Zataria multiflora and Bunium persicum essential oils as two natural antioxidants. J. Med. Plants. 2012; 1:8-21.
  37. Alizadeh A., Aghaee Z. Essential oil constituents, phenolic content and antioxidant activity of Lavandula stricta Delile growing wild in southern Iran. Nat. Prod. Res. 2016;30: 2253-2257.
  38. Abdollahzadeh E., Rezaei M., Hosseini H., Safari R. Effects of nisin and thyme essential oil, individually and in combination, on inoculated populations of Listeria monocytogenes in minced silver carp. Iranian Journal of Nutrition Sci & Food Technology. 2012; 6:13-20.
  39. Hadipanah A., Golparvar A.R., Ghasemi Pirbalouti A., Zaynali H. Determine optimum of harvest time on the quantity/quality of essential oil and thymol of thyme (Thymus vulgaris L.) in Isfahan. J Herbal Drugs. 2011;2: 23-32.
  40. Castilho P.C., Savluchinske-Feio S., Weinhold T.S., Gouveia S.C. Evaluation of the antimicrobial and antioxidant activities of essential oils, extracts and their main components from oregano from Madeira Island, Portugal. Food Control. 2012;23: 552-558.
  41. Messaoud C., Boussaid M. Myrtus communis berry color morphs: a comparative analysis of essential oils, fatty acids, phenolic compounds, and antioxidant activities. Chem. Biodivers. 2011;8: 300-310.
  42. Nantitanon W., Yotsawimonwat S., Okonogi S. Factors influencing antioxidant activities and total phenolic content of guava leaf extract. LWT-Food Sci. Technol. 2010;43: 1095-1103.
  43. Soltan Dallal M.M., Bayat M., Yazdi M.H., Aghaamiri S., Ghorbanzadeh Meshkani M., Peymaneh Abedi Mohtasab T., Shojaee Sadi B. Antimicrobial effect of Zataria multiflora on antibiotic-resistant Staphylococcus aureus strains isolated from food. SJKU. 2012; 17:21.
  44. Nasiri E. Moosavi-Nasab M., Shekarforoush S., Golmakani M. The effects of Zataria multiflora on inhibition of polyphenoloxidase and melanosis formation in shrimp (Litopenaeus vannamei. Isfj. 2014;23: 109-118.
  45. Nozohor Y., Rasolifard M.H., Ghahremanigermi N. Evaluation of Antibacterial Properties of Oregano Essence on Pathogenic Bacteria Isolated from Hospital Infections. scientific J ilam university of Medical Sci. 2018;25:154-160.
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